Publication
Absence of Gim proteins, but not GimC complex, alters stress-induced transcription
| dc.contributor.author | Amorim, Ana Fátima | |
| dc.contributor.author | Pinto, Dora | |
| dc.contributor.author | Kuras, Laurent | |
| dc.contributor.author | Fernandes, Lisete | |
| dc.date.accessioned | 2017-08-21T15:00:48Z | |
| dc.date.available | 2017-08-21T15:00:48Z | |
| dc.date.issued | 2017-07 | |
| dc.description.abstract | Saccharomyces cerevisiae GimC (mammalian Prefoldin) is a hexameric (Gim1-6) cytoplasmic complex involved in the folding pathway of actin/tubulin. In contrast to a shared role in GimC complex, we show that absence of individual Gim proteins results in distinct stress responses. No concomitant alteration in F-actin integrity was observed. Transcription of stress responsive genes is altered in gim2Δ, gim3Δ and gim6Δ mutants: TRX2 gene is induced in these mutants but with a profile diverging from type cells, whereas CTT1 and HSP26 fail to be induced. Remaining gimΔ mutants display stress transcript abundance comparable to wild type cells. No alteration in the nuclear localization of the transcriptional activators for TRX2 (Yap1) and CTT1/HSP26 (Msn2) was observed in gim2Δ. In accordance with TRX2 induction, RNA polymerase II occupancy at TRX2 discriminates the wild type from gim2Δ and gim6Δ. In contrast, RNA polymerase II occupancy at CTT1 is similar in wild type and gim2Δ, but higher in gim6Δ. The absence of active RNA polymerase II at CTT1 in gim2Δ, but not in wild type and gim1Δ, explains the respective CTT1 transcript outputs. Altogether our results put forward the need of Gim2, Gim3 and Gim6 in oxidative and osmotic stress activated transcription; others Gim proteins are dispensable. Consequently, the participation of Gim proteins in activated-transcription is independent from the GimC complex. | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | Amorim AF, Pinto D, Kuras L, Fernandes L. Absence of Gim proteins, but not GimC complex, alters stress-induced transcription. Biochim Biophys Acta (BBA). 2017;1860(7):773-81. | pt_PT |
| dc.identifier.doi | 10.1016/j.bbagrm.2017.04.005 | pt_PT |
| dc.identifier.uri | http://hdl.handle.net/10400.21/7337 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | Elsevier | pt_PT |
| dc.relation.publisherversion | http://www.sciencedirect.com/science/article/pii/S1874939917301311 | pt_PT |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | pt_PT |
| dc.subject | Transcription regulation | pt_PT |
| dc.subject | Gim proteins | pt_PT |
| dc.subject | Stress | pt_PT |
| dc.title | Absence of Gim proteins, but not GimC complex, alters stress-induced transcription | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 781 | pt_PT |
| oaire.citation.issue | 7 | pt_PT |
| oaire.citation.startPage | 773 | pt_PT |
| oaire.citation.title | BBA - Gene Regulatory Mechanisms | pt_PT |
| oaire.citation.volume | 1860 | pt_PT |
| rcaap.rights | restrictedAccess | pt_PT |
| rcaap.type | article | pt_PT |
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