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Inhibition of Aspergillus fumigatus and its biofilm by Pseudomonas aeruginosa is dependent on the source, phenotype and growth conditions of the bacterium

2015-08Journal article Open access
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dc.contributor.authorFerreira, José A. G.
dc.contributor.authorPenner, John C.
dc.contributor.authorMoss, Richard B.
dc.contributor.authorHaagensen, Janus A.
dc.contributor.authorClemons, Karl V.
dc.contributor.authorSpormann, Alfred M.
dc.contributor.authorNazik, Hasan
dc.contributor.authorCohen, Kevin
dc.contributor.authorBanaei, Niaz
dc.contributor.authorCarolino, Elisabete
dc.contributor.authorStevens, David A.
dc.date.accessioned2015-08-21T16:54:11Z
dc.date.available2015-08-21T16:54:11Z
dc.date.issued2015-08
dc.description.abstractAspergillus fumigatus (Af) and Pseudomonas aeruginosa (Pa) are leading fungal and bacterial pathogens, respectively, in many clinical situations. Relevant to this, their interface and co-existence has been studied. In some experiments in vitro, Pa products have been defined that are inhibitory to Af. In some clinical situations, both can be biofilm producers, and biofilm could alter their physiology and affect their interaction. That may be most relevant to airways in cystic fibrosis (CF), where both are often prominent residents. We have studied clinical Pa isolates from several sources for their effects on Af, including testing involving their biofilms. We show that the described inhibition of Af is related to the source and phenotype of the Pa isolate. Pa cells inhibited the growth and formation of Af biofilm from conidia, with CF isolates more inhibitory than non-CF isolates, and non-mucoid CF isolates most inhibitory. Inhibition did not require live Pa contact, as culture filtrates were also inhibitory, and again non-mucoid>mucoid CF>non-CF. Preformed Af biofilm was more resistant to Pa, and inhibition that occurred could be reproduced with filtrates. Inhibition of Af biofilm appears also dependent on bacterial growth conditions; filtrates from Pa grown as biofilm were more inhibitory than from Pa grown planktonically. The differences in Pa shown from these different sources are consistent with the extensive evolutionary Pa changes that have been described in association with chronic residence in CF airways, and may reflect adaptive changes to life in a polymicrobial environment.por
dc.identifier.citationFerreira JA, Penner JC, Moss RB, Haagensen JA, Clemons KV, Carolino E, et al. Inhibition of Aspergillus fumigatus and its biofilm by Pseudomonas aeruginosa is dependent on the source, phenotype and growth conditions of the bacterium. PLoS One. 2015;10(8):e0134692.por
dc.identifier.doi10.1371/journal.pone.0134692
dc.identifier.urihttp://hdl.handle.net/10400.21/4919
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherPLoSpor
dc.relation.publisherversionhttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0134692por
dc.subjectAspergillus fumigatuspor
dc.subjectPseudomonas aeruginosapor
dc.subjectPathogenspor
dc.subjectFungipor
dc.subjectBacteriapor
dc.subjectCystic fibrosispor
dc.titleInhibition of Aspergillus fumigatus and its biofilm by Pseudomonas aeruginosa is dependent on the source, phenotype and growth conditions of the bacteriumpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.startPagee0134692por
oaire.citation.titlePLoS ONEpor
oaire.citation.volume10por
person.familyNameCarolino
person.givenNameElisabete
person.identifier.ciencia-id1216-EFA3-1E0F
person.identifier.orcid0000-0003-4165-7052
person.identifier.ridF-1012-2015
person.identifier.scopus-author-id25821697000
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication77930d39-ed34-44dc-a4a6-9bf833e5e688
relation.isAuthorOfPublication.latestForDiscovery77930d39-ed34-44dc-a4a6-9bf833e5e688

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