Repository logo
 
Publication

Genotoxicity and cytotoxicity assessment of volatile organic compounds in pathology professionals through the buccal micronuclei assay

2025-05Journal article Open access
Simple item page
datacite.subject.sdg03:Saúde de Qualidade
dc.contributor.authorBaptista, Fátima
dc.contributor.authorGarcia, Patrícia V.
dc.contributor.authorRodrigues, Armindo S.
dc.contributor.authorLadeira, Carina
dc.date.accessioned2025-06-04T15:19:28Z
dc.date.available2025-06-04T15:19:28Z
dc.date.issued2025-05
dc.descriptionThis research was funded by cE3c (DOI: 10.54499/UIDB/00329/2020), IVAR (DOI: 10.54499/UIDP/00643/2020), H&TRC FCT/MCTES UIDP/05608/2020 (DOI: 10.54499/UIDP/05608/2020), and UIDB/05608/2020 (DOI: 10.54499/UIDB/05608/2020).
dc.description.abstractIn pathology laboratories, several volatile organic compounds (VOCs) are used, such as formaldehyde, ethanol, and xylene. These substances are recognized as genotoxic and cytotoxic, which is why their handling poses risks to human health. The buccal micronucleus (MN) cytome assay is a non-invasive, useful, and simple method to detect these effects in exposed individuals. The study aimed to evaluate the risk of genotoxicity and cytotoxicity of VOCs in pathology professionals of S. Miguel Island, Azores, Portugal. The study comprised two groups: exposed workers (n = 21) from the three laboratories of S. Miguel, and a reference group (n = 50), randomly chosen from other hospital services without known exposure to VOCs. The exfoliated buccal cells were auto-sampled by all the participants using a cytobrush. The samples were processed in ThinPrep®, stained with modified Feulgen with Fast Green, and visualized for MN and other nuclear anomalies (ONAs), such as karyorrhexis, pyknotic, and karyolytic cells. Results showed that VOCs have a predictive significance for MN frequency, leading to the conclusion that their exposure is an increased risk factor for the health of these professionals, approximately four times greater than in the control group.eng
dc.identifier.citationBaptista F, Garcia PV, Rodrigues AS, Ladeira C. Genotoxicity and cytotoxicity assessment of volatile organic compounds in pathology professionals through the buccal micronuclei assay. Toxics. 2025;13(5):411.
dc.identifier.doi10.3390/toxics13050411
dc.identifier.issn2305-6304
dc.identifier.urihttp://hdl.handle.net/10400.21/21905
dc.language.isoeng
dc.peerreviewedyes
dc.publisherMDPI AG
dc.relation.hasversionhttps://www.mdpi.com/2305-6304/13/5/411
dc.relation.ispartofToxics
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectDNA damage
dc.subjectBuccal mucosa cells
dc.subjectMicronuclei
dc.subjectOccupational exposure
dc.subjectPathology lab
dc.subjectVolatile organic compounds
dc.subjectFCT_UIDP/05608/2020
dc.subjectFCT_UIDB/05608/2020
dc.titleGenotoxicity and cytotoxicity assessment of volatile organic compounds in pathology professionals through the buccal micronuclei assayeng
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue5
oaire.citation.startPage411
oaire.citation.titleToxics
oaire.citation.volume13
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameLadeira
person.givenNameCarina
person.identifier144237
person.identifier.ciencia-id801C-1BBA-1D9E
person.identifier.orcid0000-0001-5588-0074
person.identifier.ridJ-2572-2012
person.identifier.scopus-author-id36463788000
relation.isAuthorOfPublication1aef4b60-4197-436b-84ab-80d31cbaed33
relation.isAuthorOfPublication.latestForDiscovery1aef4b60-4197-436b-84ab-80d31cbaed33

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Genotoxicity and cytotoxicity assessment of volatile organic compounds in pathology professionals through the buccal micronuclei assay.pdf
Size:
660.58 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
4.03 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

ESTeSL - Artigos