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Microbiological contamination assessment in higher education institutes

2021-08Journal article Open access
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dc.contributor.authorViegas, Carla
dc.contributor.authorPimenta, Raquel
dc.contributor.authorDias, Marta
dc.contributor.authorGomes, Bianca
dc.contributor.authorBrito, Miguel
dc.contributor.authorCaetano, Liliana Aranha
dc.contributor.authorCarolino, Elisabete
dc.contributor.authorGomes, Anita Q.
dc.date.accessioned2021-09-02T17:39:15Z
dc.date.available2021-09-02T17:39:15Z
dc.date.issued2021-08
dc.descriptionIPL_MOMENTO ZERO (ESTeSL/IPL/2020)pt_PT
dc.descriptionFCT_UIDB/05608/2020pt_PT
dc.descriptionFCT_UIDP/05608/2020pt_PT
dc.description.abstractThe higher education sector represents a unique environment and it acts as a work environment, a learning environment for students, and frequently, also a home environment. The aim of this study was to determine the microbial contamination (SARS-CoV-2, fungi, and bacteria) in Higher Education Facilities (HEI) by using active and passive sampling methods and combining culture-based methods with molecular tools targeting Aspergillus section Fumigati. In addition, the resistance to azole profile was also assessed. Surface samples showed a range of total bacterial contamination between 1 × 103 to 3.1 × 106 CFU·m−2, while Gram-negative bacteria ranged from 0 to 1.9 × 104 CFU·m−2. Fungal contamination ranged from 2 × 103 to 1.8 × 105 CFU·m−2 on MEA and from 5 × 103 to 1.7 × 105 CFU·m−2 on DG18. The most prevalent species found on both media was Cladosporium sp. (47.36% MEA; 32.33% DG18). Aspergillus genera were observed on MEA (3.21%) and DG18 (14.66%), but not in the supplemented media used for the azole screening. Aspergillus section Fumigati was detected in 2 air samples (2.22%, 2 out of 90 samples) by qPCR. When testing for SARS-CoV-2 all results were negative. The present study showed that although cleaning and disinfection procedures are done regularly due to the COVID-19 pandemic, being effective in eliminating SARS-CoV-2, surfaces were often contaminated with microorganisms other than SARS-CoV-2. This can be a result of increasing resistance to biocides, and to the wide range of environmental factors that can contribute to the dissemination of microbial contamination indoors.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationViegas C, Pimenta R, Brito M, Caetano LA, Carolino E, Gomes AQ, et al. Microbiological contamination assessment in higher education institutes. Atmosphere. 2021;12(8):1079.pt_PT
dc.identifier.doi10.3390/atmos12081079pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/13668
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationProject IPL_MOMENTO ZERO (ESTeSL/IPL/2020)pt_PT
dc.relationFCT_UIDB/05608/2020pt_PT
dc.relationFCT_UIDP/05608/2020pt_PT
dc.relation.publisherversionhttps://www.mdpi.com/2073-4433/12/8/1079pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectOccupational healthpt_PT
dc.subjectOccupational exposurept_PT
dc.subjectMicrobiological contaminationpt_PT
dc.subjectActive and passive samplingpt_PT
dc.subjectFungipt_PT
dc.subjectBacteriapt_PT
dc.subjectAzole resistance screeningpt_PT
dc.subjectAspergillus spppt_PT
dc.subjectAspergillus fumigatuspt_PT
dc.subjectIPL_MOMENTO ZERO (ESTeSL/IPL/2020)pt_PT
dc.subjectFCT_UIDB/05608/2020pt_PT
dc.subjectFCT_UIDP/05608/2020pt_PT
dc.titleMicrobiological contamination assessment in higher education institutespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue8pt_PT
oaire.citation.startPage1079pt_PT
oaire.citation.titleAtmospherept_PT
oaire.citation.volume12pt_PT
person.familyNameViegas
person.familyNameBrito
person.familyNameAranha Caetano
person.familyNameCarolino
person.familyNameGomes
person.givenNameCarla
person.givenNameMiguel
person.givenNameLiliana
person.givenNameElisabete
person.givenNameAnita
person.identifier.ciencia-idEE1E-C639-D70F
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person.identifier.ciencia-id9716-9DAC-532A
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person.identifier.ciencia-id4B10-E015-52B7
person.identifier.orcid0000-0002-1545-6479
person.identifier.orcid0000-0001-6394-658X
person.identifier.orcid0000-0003-1496-2609
person.identifier.orcid0000-0003-4165-7052
person.identifier.orcid0000-0002-3348-0448
person.identifier.ridB-7217-2013
person.identifier.ridA-7970-2016
person.identifier.ridF-1012-2015
person.identifier.ridC-3580-2014
person.identifier.scopus-author-id55443609700
person.identifier.scopus-author-id35224551000
person.identifier.scopus-author-id25821697000
person.identifier.scopus-author-id7202386033
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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