Browsing by Issue Date, starting with "2021-08"
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- DNA damage in circulating leukocytes measured with the comet assay may predict the risk of deathPublication . Bonassi, Stefano; Ceppi, Marcello; Møller, Peter; Azqueta, Amaya; Milić, Mirta; Monica, Neri; Brunborg, Gunnar; Godschalk, Roger; Koppen, Gudrun; Langie, Sabine A. S.; Teixeira, João Paulo; Bruzzone, Marco; Da Silva, Juliana; Benedetti, Danieli; Cavallo, Delia; Ursini, Cinzia Lucia; Giovannelli, Lisa; Moretti, Silvia; Riso, Patrizia; Del Bo’, Cristian; Russo, Patrizia; Dobrzyńska, Malgorzata; Goroshinskaya, Irina A.; Surikova, Ekaterina I.; Staruchova, Marta; Barančokova, Magdalena; Volkovova, Katarina; Kažimirova, Alena; Smolkova, Bozena; Laffon, Blanca; Valdiglesias, Vanessa; Pastor, Susana; Marcos, Ricard; Hernández, Alba; Gajski, Goran; Spremo-Potparević, Biljana; Živković, Lada; Boutet-Robinet, Elisa; Perdry, Hervé; Lebailly, Pierre; Perez, Carlos L.; Basaran, Nursen; Nemeth, Zsuzsanna; Safar, Anna; Dusinska, Maria; Collins, Andrew; Anderson, Diana; Andrade, Vanessa; Pereira, Cristiana Costa; Costa, Solange; Gutzkow, Kristine B.; Ladeira, Carina; Moretti, Massimo; Costa, Carla; Orlow, Irene; Rojas, Emilio; Pourrut, Bertrand; Kruszewski, Marcin; Knasmueller, Siegfried; Shaposhnikov, Sergey; Žegura, Bojana; Stopper, HelgaThe comet assay or single cell gel electrophoresis is the most common method used to measure strand breaks and a variety of other DNA lesions in human populations. To estimate the risk of overall mortality, mortality by cause, and cancer incidence associated with DNA damage, a cohort of 2,403 healthy individuals (25,978 person-years) screened in 16 laboratories using the comet assay between 1996 and 2016 was followed-up. Kaplan–Meier analysis indicated a worse overall survival in the medium and high tertile of DNA damage (p < 0.001). The effect of DNA damage on survival was modeled according to Cox proportional hazard regression model. The adjusted hazard ratio (HR) was 1.42 (1.06–1.90) for overall mortality and 1.94 (1.04–3.59) for diseases of the circulatory system in subjects with the highest tertile of DNA damage. The findings of this study provide epidemiological evidence encouraging the implementation of the comet assay in preventive strategies for non-communicable diseases.
- Resistance element welding of sandwich laminates with hidden insertsPublication . Calado, Francisco N.; Pragana, João; Bragança, Ivo; Silva, Carlos M. A.; Martins, Paulo A. F.This paper presents a new resistance element welding process capable of producing invisible lap joints between steel-polymer-steel composite laminates. The process involves pre-drilling a flat-bottom hole in each laminate to remove the polymer core and one of the steel sheets, and positioning a cylindrical insert inside the two adjoining holes for subsequent resistance welding. Finite element modeling is utilized to construct the weldability lobe and to identify the parameters that lead to the formation of acceptable joints. Experimental results confirm the applicability of the process to produce invisible lap joints without signs of material protrusions or local indentations resulting from squeezing the polymer out to create contact between the steel sheets. Destructive peel and shear tests allow determining the maximum forces that the joints can safely withstand and comparing their performance against alternative joined by forming lap joints in which the mechanical interlocking is also hidden inside the laminates.
- Electric performance of fastened hybrid busbars: An experimental and numerical studyPublication . Sampaio, Rui F. V.; Pragana, João P. M.; Bragança, Ivo; Silva, Carlos M. A .; Nielsen, Chris Valentin; Martins, P.A.F.This paper is focused on fastened hybrid busbars made from copper and aluminium with the purpose of analysing the influence of the steel bolts, of their tightening torque and of the surface condition of the sheets on the electric current flow and electric resistance of the joints. The methodology combines experimentation with unit cells that are representative of the joints and electro-mechanical numerical simulation using a finite element computer program developed by the authors. Results are a step forward in understanding the combined influence of bolts, contact pressure and surface roughness on the electric performance of fastened hybrid busbars. Design guidelines for dimensioning the cross sections of the copper and aluminium sheets and for effectively distributing bolts across the contacting surfaces are also provided.
- Microbiological contamination assessment in higher education institutesPublication . Viegas, Carla; Pimenta, Raquel; Dias, Marta; Gomes, Bianca; Brito, Miguel; Caetano, Liliana Aranha; Carolino, Elisabete; Gomes, Anita Q.The higher education sector represents a unique environment and it acts as a work environment, a learning environment for students, and frequently, also a home environment. The aim of this study was to determine the microbial contamination (SARS-CoV-2, fungi, and bacteria) in Higher Education Facilities (HEI) by using active and passive sampling methods and combining culture-based methods with molecular tools targeting Aspergillus section Fumigati. In addition, the resistance to azole profile was also assessed. Surface samples showed a range of total bacterial contamination between 1 × 103 to 3.1 × 106 CFU·m−2, while Gram-negative bacteria ranged from 0 to 1.9 × 104 CFU·m−2. Fungal contamination ranged from 2 × 103 to 1.8 × 105 CFU·m−2 on MEA and from 5 × 103 to 1.7 × 105 CFU·m−2 on DG18. The most prevalent species found on both media was Cladosporium sp. (47.36% MEA; 32.33% DG18). Aspergillus genera were observed on MEA (3.21%) and DG18 (14.66%), but not in the supplemented media used for the azole screening. Aspergillus section Fumigati was detected in 2 air samples (2.22%, 2 out of 90 samples) by qPCR. When testing for SARS-CoV-2 all results were negative. The present study showed that although cleaning and disinfection procedures are done regularly due to the COVID-19 pandemic, being effective in eliminating SARS-CoV-2, surfaces were often contaminated with microorganisms other than SARS-CoV-2. This can be a result of increasing resistance to biocides, and to the wide range of environmental factors that can contribute to the dissemination of microbial contamination indoors.
- Characterization of airborne emission of nanoparticles in the ceramic industry in PortugalPublication . Esteves, Hélder; Bordado, J.; Gomes, João; Miranda, R.; Albuquerque, PaulaThe objective of this study was to evaluate occupational exposure to nanoparticles during some tasks performed in different production processes of different ceramic industries in Portugal, to select the places of greatest occupational exposure through the analysis of the sampled data, to verify what is the pulmonary accumulation in these places, to identify the composition of the released nanoparticles, apply a Control Banding Tool and try to understand which companies require more risk control measures. The study was carried out in three different national ceramics production industries, one for sanitary ceramics production, another for porcelain crockery production and finally another for the production of ornamental crockery (red paste). It is concluded that occupational exposure values to nanoparticles are high in all cases and that nanoparticles are very small in size (11.5 to 15.4 nm). Existing risk control measures are insufficient and verified risk levels are high (Risk Level 3 and 4). The chemical composition of the analyzed nanoparticles is similar regardless of the typology of the ceramic production plant and their chemical composition as a percentage of certain materials has a direct influence on crystallinity.
- Assessment of wireless charging impacts based on real-world driving patterns: Case study in Lisbon, PortugalPublication . Duarte, Gonçalo; Silva, André; Baptista, PatríciaIn the current transition to a smarter and more efficient transportation system, battery electric vehicle mileage and the time required for charging are still two main constraints that need to be overcome to enable a larger penetration of electric vehicles. Moreover, the few charging stations available are a consequence of the "supply and demand" problem. Consequently, wireless dynamic recharging can be a complementary solution to address the problems of light-duty electric mobility and an added-value towards autonomous vehicles. Consequently, this paper presents an innovative approach based on real world mobility patterns collected for a sample in the city of Lisbon, Portugal, to assess users' electric vehicle feasibility by assessing different recharging scenarios, comparing stationary and dynamic recharging scenarios. The results indicate that at least 15 % more drivers would be eligible to own an electric vehicle if wireless charging was available. Moreover, wireless charging reduces the range of battery used, with stationary charging requiring circa 3.2 times more battery range. The developed approach confirms that wireless dynamic recharging can significantly change the framework of current electric mobility limitations, reducing range anxiety issues, contributing to redesign electric vehicle battery capacity and overcome barriers in stationary charging deployment and availability.
- Impact of perioperative chemotherapy on postoperative morbidity after gastrectomy for gastric cancerPublication . Cabral, Francisco; Ramos, Paulo; Monteiro, Cecília; Casaca, Rui; Pinto, Iola; Abecasis, NunoIntroduction: The use of perioperative chemotherapy (CT) in patients with advanced gastric carcinoma increases their overall survival. This therapy may also increase the number of patients with R0 resection. Potential drawbacks of this therapy, besides its toxicity, include increased surgical morbidity. Methods: We retrospectively evaluated the records of patients undergoing gastrectomy with curative intent, for carcinoma, at our institution between January 2009 and August 2018. They were divided into two groups: direct surgery (SURG) and perioperative CT (CHEMO). Patients with other neoadjuvant therapies and cardia Siewert I and II carcinomas were excluded. The primary objective was to evaluate the impact of perioperative CT on surgical morbidity. As secondary objectives, resection radicality and total lymph node count were compared between the two groups. Results: A total of 307 patients (97 direct surgery and 210 perioperative CT) were evaluated. Median age was 67 years old. The overall major surgical morbidity (Clavien-Dindo 3-5) was 10.6% in the CHEMO group and 12.4 in the SURG group (p = 0.643). There was no statistically significant difference between the surgical radicality (R0 98% in the SURG group vs 97.5% CHEMO group (p = 0.865). There was an increase in the total number of lymph nodes retrieved in the specimen in the CHEMO group (25 vs 22, p = 0.001), a difference that was not maintained in the subgroup analysis as a function of the surgery performed. Conclusions: Perioperative CT in gastric carcinoma does not increase surgical morbidity, surgical radicality and total lymph node count. (C) 2020 AEC. Published by Elsevier Espana, S.L.U. All rights reserved.
- Biomonitoring of occupational exposure to bisphenol A, bisphenol S and bisphenol F: a systematic reviewPublication . Bousoumah, Radia; Leso, Veruscka; Iavicoli, Ivo; Huuskonen, Pasi; Viegas, Susana; Porras, Simo P.; Santonen, Tiina; Frery, Nadine; Robert, Alain; Ndaw, SophieBisphenol A (BPA) and its substitutes bisphenol S (BPS) and bisphenol F (BPF) are endocrine disrupting chemicals widely used in the production of polycarbonate plastics, epoxy resins and thermal papers. The aim of the review was to identify occupational studies using human biomonitoring (HBM) as a tool for bisphenol exposure assessment and to characterize research gaps on the topic as part of the HBM4EU project. Hence, a systematic literature search using the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) methodology was conducted for articles published between 2000 and 27th March 2020 across three databases (PubMed, Scopus and Web of Science). Thirty studies on the occupational HBM of BPA met the inclusion criteria. Regarding BPS and BPF, only 4 and 2 publications were retrieved, respectively. Fifty-seven percent (57%) of the studies selected for BPA were conducted in Asia whereas half of BPS and BPF studies were undertaken in Europe. Studies on BPA in plastic and epoxy resin sectors were infrequent in Europe while Asian data showed higher exposure when the substance is employed as raw material. The main data on BPS were among cashiers while BPF data were available from incinerator workers. Several research gaps have been identified: (i) shortage of HBM studies on occupational exposure, especially to BPS and BPF; (ii) different methodological designs making suitable comparisons between studies difficult; and (iii) only few studies conducted on the industrial applications of bisphenols outside Asia. This review highlights the lack of recent occupational HBM studies on bisphenols and the need for a harmonized approach to acquire reliable data. Considering the increasing replacement of BPA by BPS and BPF, it is of relevance to evaluate the exposure to these substances and the impact of the available risk management measures on workers exposure and possible health risk.
- Partial automorphisms and injective partial endomorphisms of a finite undirected pathPublication . Dimitrova, Ilinka; Fernandes, V. H.; Koppitz, J.; Quinteiro, TeresaIn this paper, we study partial automorphisms and, more generally, injective partial endomorphisms of a finite undirected path from Semigroup Theory perspective. Our main objective is to give formulas for the ranks of themonoids IEnd(P-n) and PAut(P-n) of all injective partial endomorphisms and of all partial automorphisms of the undirected path Pn with n vertices. We also describe Green's relations of PAut(P-n) and IEnd(P-n) and calculate their cardinals.
- Crack onset in stretched open hole PMMA plates considering linear and non-linear elastic behavioursPublication . Leite, Afonso; Mantic, V.; Paris, F.Crack onset in PMMA holed plates subjected to tensile stresses is studied experimentally and by the coupled stress and energy criterion of the Finite Fracture Mechanics (CCFFM). The elastic, strength and fracture properties of PMMA are determined by the standard tests, a clearly nonlinear stress-strain relation being identified in the tensile tests. Thus, a novel numerical implementation of the CCFFM considering a non-linear elastic (NLE) material model, using the Ramberg-Osgood approximation, in addition to the usually used linear elastic (LE) model, is developed. Testing of plates with different hole sizes shows a hole size effect in the nominal failure load as expected. For a better fitting of the experimental results, higher strength values obtained by three point bending (TPB) flatwise and edgewise coupons (without any notch), for these material models, are used, apparently for the first time, in the CCFFM predictions. This approach reflects the observation that the strength values associated to smaller but highly stressed volumes, like those located at stress maxima in the holed plates and TPB specimens, are higher. For finite-width holed plates and both material behaviours, suitable FEM models are developed to implement the CCFFM for both LE and NLE models, considering plane stress state. Moreover, an inverse procedure is devised, using the experimental data for holed plates and predictions by CCFFM, to estimate the strength and fracture properties to be used in both material models, providing very good correlations of the CCFFM predictions with the experimental results.