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A new method to predict genotoxic effects based on serum molecular profile

2021-03Journal article Open access
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dc.contributor.authorAraújo, Rúben
dc.contributor.authorRamalhete, Luís
dc.contributor.authorPaz, Hélder
dc.contributor.authorLadeira, Carina
dc.contributor.authorCalado, Cecília
dc.date.accessioned2021-03-19T20:15:00Z
dc.date.available2024-03-19T01:30:18Z
dc.date.issued2021-03
dc.descriptionIDI&CA/IPL/2017/GenTox/ESTeSLpt_PT
dc.description.abstractIt is critical to developing new methods to assess genotoxic effects in human biomonitoring since conventional methods are usually laborious, time-consuming, and expensive. It is aimed to evaluate if the analysis of a drop of serum by Fourier Transform Infrared spectroscopy, allows assessing genotoxic effects in occupational exposure to cytostatic drugs in hospital professionals, as obtained by the lymphocyte cytokinesis-block micronucleus assay. It was considered peripheral blood from hospital professionals exposed to cytostatic drugs (n = 22) and from a non-exposed group (n = 36). It was observed that workers occupationally exposed presented a higher number of micronuclei (p < 0.05) in lymphocytes, in relation to the non-exposed group. The serum Fourier Transform Infrared spectra from exposed workers presented diverse different peaks (p < 0.01) in relation to the non-exposed group. The hierarchical cluster analysis of serum spectra separated serum samples of the exposed group from the non-exposed group with 61% sensitivity and 88% specificity. A support vector machine model of serum spectra enables the prediction of exposure with high accuracy (0.91), precision (0.89), sensitivity (0.86), F1 score (0.87), and AUC (0.96). Therefore, Fourier Transform Infrared spectroscopic analysis of a drop of serum enabled to predict in a rapid and simple mode the genotoxic effects of cytostatic drugs. The method presents therefore potential for high-dimension screening of exposure to genotoxic substances, due to its simplicity and rapid setup mode.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAraújo R, Ramalhete L, Paz H, Ladeira C, Calado CR. A new method to predict genotoxic effects based on serum molecular profile. Spectrochim Acta A Mol Biomol Spectroscop. 2021;255:119680.pt_PT
dc.identifier.doi10.1016/j.saa.2021.119680pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/13125
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationIDI&CA/IPL/2017/GenTox/ESTeSLpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S1386142521002560pt_PT
dc.subjectBiomonitoringpt_PT
dc.subjectGenotoxicitypt_PT
dc.subjectSerumpt_PT
dc.subjectCBMNpt_PT
dc.subjectFTIR spectroscopypt_PT
dc.subjectIDI&CA/IPL/2017/GenTox/ESTeSLpt_PT
dc.titleA new method to predict genotoxic effects based on serum molecular profilept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.startPage119680pt_PT
oaire.citation.titleSpectrochimica Acta Part A: Molecular and Biomolecular Spectroscopypt_PT
oaire.citation.volume255pt_PT
person.familyNameLadeira
person.familyNameCalado
person.givenNameCarina
person.givenNameCecília
person.identifier144237
person.identifier130332
person.identifier.ciencia-id801C-1BBA-1D9E
person.identifier.ciencia-id9418-E320-3177
person.identifier.orcid0000-0001-5588-0074
person.identifier.orcid0000-0002-5264-9755
person.identifier.ridJ-2572-2012
person.identifier.ridE-2102-2014
person.identifier.scopus-author-id36463788000
person.identifier.scopus-author-id6603163260
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication1aef4b60-4197-436b-84ab-80d31cbaed33
relation.isAuthorOfPublicatione8577257-c64c-4481-9b2b-940fedb360cc
relation.isAuthorOfPublication.latestForDiscovery1aef4b60-4197-436b-84ab-80d31cbaed33

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