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Advisor(s)
Abstract(s)
It is critical to developing new methods to assess genotoxic effects in human biomonitoring since conventional methods are usually laborious, time-consuming, and expensive. It is aimed to evaluate if the analysis of a drop of serum by Fourier Transform Infrared spectroscopy, allows assessing genotoxic effects in occupational exposure to cytostatic drugs in hospital professionals, as obtained by the lymphocyte cytokinesis-block micronucleus assay. It was considered peripheral blood from hospital professionals exposed to cytostatic drugs (n = 22) and from a non-exposed group (n = 36). It was observed that workers occupationally exposed presented a higher number of micronuclei (p < 0.05) in lymphocytes, in relation to the non-exposed group. The serum Fourier Transform Infrared spectra from exposed workers presented diverse different peaks (p < 0.01) in relation to the non-exposed group. The hierarchical cluster analysis of serum spectra separated serum samples of the exposed group from the non-exposed group with 61% sensitivity and 88% specificity. A support vector machine model of serum spectra enables the prediction of exposure with high accuracy (0.91), precision (0.89), sensitivity (0.86), F1 score (0.87), and AUC (0.96). Therefore, Fourier Transform Infrared spectroscopic analysis of a drop of serum enabled to predict in a rapid and simple mode the genotoxic effects of cytostatic drugs. The method presents therefore potential for high-dimension screening of exposure to genotoxic substances, due to its simplicity and rapid setup mode.
Description
IDI&CA/IPL/2017/GenTox/ESTeSL
Keywords
Biomonitoring Genotoxicity Serum CBMN FTIR spectroscopy IDI&CA/IPL/2017/GenTox/ESTeSL
Citation
Araújo R, Ramalhete L, Paz H, Ladeira C, Calado CR. A new method to predict genotoxic effects based on serum molecular profile. Spectrochim Acta A Mol Biomol Spectroscop. 2021;255:119680.