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Impact of a training course on the quality of malaria diagnosis by microscopy in Angola

2014-11Journal article Open access
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dc.contributor.authorMoura, Sofia
dc.contributor.authorFançony, Cláudia
dc.contributor.authorMirante, Clara
dc.contributor.authorNeves, Marcela
dc.contributor.authorBernardino, Luís
dc.contributor.authorFortes, Filomeno
dc.contributor.authorSambo, Maria do Rosário
dc.contributor.authorBrito, Miguel
dc.date.accessioned2015-08-13T10:32:50Z
dc.date.available2015-08-13T10:32:50Z
dc.date.issued2014-11
dc.description.abstractBackground: In Angola, malaria is an endemic disease having a major impact on the economy. The WHO recommends testing for all suspected malaria cases, to avoid the presumptive treatment of this disease. In malaria endemic regions laboratory technicians must be very comfortable with microscopy, the golden standard for malaria diagnosis, to avoid the incorrect diagnosis. The improper use of medication promotes drug resistance and undesirable side effects. The present study aims to assess the impact of a three-day refresher course on the knowledge of technicians, quality of blood smears preparation and accuracy of microscopy malaria diagnosis, using qPCR as reference method. Methods: This study was implemented in laboratories from three hospitals in different provinces of Angola: Bengo, Benguela and Luanda. In each laboratory samples were collected before and after the training course (slide with thin and thick blood smears, a dried blood spot and a form). The impact of the intervention was evaluated through a written test, the quality of slide preparation and the performance of microscopy. Results: It was found a significant increase on the written test median score, from 52.5% to 65.0%. A total of 973 slides were analysed to evaluate the quality of thick and thin blood smears. Considering all laboratories there was a significant increase in quality of thick and thin blood smears. To determine the performance of microscopy using qPCR as the reference method we used 1,028 samples. Benguela presented the highest values for specificity, 92.9% and 98.8% pre and post-course, respectively and for sensitivity the best pre-course was Benguela (75.9%) and post-course Luanda (75.0%). However, no significant increase in sensitivity and specificity after the training course was registered in any laboratory analysed. Discussion: The findings of this study support the need of continuous refresher training for microscopists and other laboratory staff. The laboratories should have a quality control programme to supervise the diagnosis and also to assess the periodicity of new training. However, other variables needed to be considered to have a correct malaria diagnosis, such as adequate equipment and reagents for staining and visualization, good working conditions, motivated and qualified personnel.por
dc.identifier.citationMoura S, Fançony C, Mirante C, Neves M, Bernardino L, Brito M, et al. Impact of a training course on the quality of malaria diagnosis by microscopy in Angola. Malar J. 2014;13:437.por
dc.identifier.doi10.1186/1475-2875-13-437
dc.identifier.urihttp://hdl.handle.net/10400.21/4739
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherBioMed Centralpor
dc.relation.publisherversionhttp://www.malariajournal.com/content/13/1/437por
dc.subjectMalariapor
dc.subjectTrainingpor
dc.subjectMicroscopypor
dc.subjectQualitypor
dc.subjectProfessional competencepor
dc.subjectLaboratory personnelpor
dc.subjectHospitalpor
dc.subjectAngolapor
dc.titleImpact of a training course on the quality of malaria diagnosis by microscopy in Angolapor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.startPage437por
oaire.citation.titleMalaria Journalpor
oaire.citation.volume13por
person.familyNameBrito
person.givenNameMiguel
person.identifier.ciencia-id231F-F341-7E93
person.identifier.orcid0000-0001-6394-658X
person.identifier.ridA-7970-2016
person.identifier.scopus-author-id35224551000
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication4252d8e0-800c-4d67-8b13-0b711d860669
relation.isAuthorOfPublication.latestForDiscovery4252d8e0-800c-4d67-8b13-0b711d860669

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