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Transient middle cerebral artery occlusion in rats as a nonclinical model of ischemic stroke: a systematic review

2026-06Artigo científico Acesso aberto
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datacite.subject.sdg03:Saúde de Qualidade
dc.contributor.authorMendes, Priscila
dc.contributor.authorPinto, Joana
dc.contributor.authorMateus, Carole
dc.contributor.authorGuerra, Inês
dc.contributor.authorMateus, Vanessa
dc.date.accessioned2026-07-07T07:39:42Z
dc.date.available2026-07-07T07:39:42Z
dc.date.issued2026-06
dc.descriptionThe authors from the Research Institute for Medicines (iMed.ULisboa) gratefully acknowledge the support of the Fundação para a Ciência e a Tecnologia/Ministério da Ciência, Tecnologia e Ensino Superior (UID/04138/2025; UID/PRR/04138/2025; UID/PRR2/04138/2025).
dc.description.abstractBackground: Ischemic stroke remains a leading cause of mortality and disability worldwide. Despite extensive preclinical research, most neuroprotective strategies have failed to translate into clinical benefit, partly due to methodological variability. The transient intraluminal filament middle cerebral artery occlusion (tifMCAO) model is widely used, yet its implementation lacks consistency. This review aimed to characterize tifMCAO methodologies in adult rats and examine how experimental variability relates to reported outcomes. Methods: A systematic review was conducted following PRISMA guidelines. Studies using tifMCAO in adult rats were included. MEDLINE (PubMed), Web of Science, and Scopus were searched up to March 2025. Risk of bias was assessed using the SYRCLE tool and reporting quality using the ARRIVE checklist. The protocol was registered in PROSPERO (CRD420251140869). Results were synthesized narratively. Results: A total of 125 studies were included. A commonly used framework involved male Sprague-Dawley rats (6-12 weeks), silicone-coated monofilaments, occlusion durations of 60-120 min (most frequently 90 min), and isoflurane anesthesia, although this reflects methodological convergence rather than true standardization. Substantial variability was observed across methodological parameters. Variations in ischemia duration, filament properties, and anesthesia were associated with differences in infarct size, blood-brain barrier disruption, and functional outcomes. Conclusions: The tifMCAO model shows partial methodological convergence alongside significant variability influencing outcomes. Improved standardization and reporting are essential to enhance reproducibility and translational relevance.eng
dc.identifier.citationMendes P, Pinto J, Mateus C, Guerra I, Mateus V. Transient middle cerebral artery occlusion in rats as a nonclinical model of ischemic stroke: a systematic review. Curr Issues Mol Biol. 2026;48(6):632.
dc.identifier.doi10.3390/cimb48060632
dc.identifier.issn1467-3045
dc.identifier.urihttp://hdl.handle.net/10400.21/22945
dc.language.isoeng
dc.peerreviewedyes
dc.publisherMDPI AG
dc.relation.hasversionhttps://www.mdpi.com/1467-3045/48/6/632
dc.relation.ispartofCurrent Issues in Molecular Biology
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectExperimental model
dc.subjectIschemic stroke
dc.subjectMethodological variability
dc.subjectOutcome assessment
dc.subjectRats
dc.subjectReproducibility
dc.subjecttifMCAO
dc.subjectTransient middle cerebral artery occlusion
dc.subjectTranslational relevance
dc.subjectSystematic review
dc.titleTransient middle cerebral artery occlusion in rats as a nonclinical model of ischemic stroke: a systematic revieweng
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue6
oaire.citation.startPage632
oaire.citation.titleCurrent Issues in Molecular Biology
oaire.citation.volume48
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameRodrigues Gomes Mendes
person.familyNamePinho Mateus
person.givenNamePriscila
person.givenNameVanessa Alexandra
person.identifier.ciencia-id6A16-5C18-F10B
person.identifier.ciencia-id5A12-571D-AD6A
person.identifier.orcid0000-0002-0013-3140
person.identifier.orcid0000-0002-3204-3772
relation.isAuthorOfPublicationb02faa18-658e-4edc-b846-7c0b3646414c
relation.isAuthorOfPublication406041a5-682c-4f94-a4e2-ddbfc541313c
relation.isAuthorOfPublication.latestForDiscoveryb02faa18-658e-4edc-b846-7c0b3646414c

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