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BCG-loaded chitosan microparticles: interaction with macrophages and preliminary in vivo studies

2017-04Journal article Restricted access
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dc.contributor.authorCaetano, Liliana Aranha
dc.contributor.authorFigueiredo, Lara
dc.contributor.authorAlmeida, António J.
dc.contributor.authorGonçalves, L. M.
dc.date.accessioned2018-01-17T11:00:26Z
dc.date.available2018-01-17T11:00:26Z
dc.date.issued2017-04
dc.description.abstractThe aim of this study was to develop a novel BCG-loaded chitosan vaccine with high association efficiency which can afford efficient interaction with APC and elicit local and Th1-type-specific immune response after intranasal administration. Chitosan-suspended BCG and BCG-loaded chitosan-alginate microparticles were prepared by ionotropic gelation. Interaction with APC was evaluated by fluorescence microscopy using rBCG-GFP. Specific immune responses were evaluated following intranasal immunization of mice. Cellular uptake was approximately two-fold higher for chitosan-suspended BCG. A single dose of BCG-loaded microparticles or chitosan-suspended BCG by intranasal route improved Th1-type response compared with subcutaneous BCG. Chitosan-suspended BCG originated the highest mucosal response in the lungs by the intranasal route. These positive results indicate that the proposed approach of whole live BCG microencapsulation in chitosan-alginate for intranasal immunization was successful in allowing efficient interaction with APC while improving the cellular immune response, which is of interest for local immunization against tuberculosis.pt_PT
dc.description.sponsorshipESTeSL-IPL/CGD/2012pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCaetano LA, Figueiredo L, Almeida AJ, Gonçalves LM. BCG-loaded chitosan microparticles: interaction with macrophages and preliminary in vivo studies. J Microencapsul. 2017;24(2):203-17.pt_PT
dc.identifier.doi10.1080/02652048.2017.1316325pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/7928
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherTaylor & Francispt_PT
dc.relation.publisherversionhttp://www.tandfonline.com/doi/abs/10.1080/02652048.2017.1316325pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectAdministration, Intranasalpt_PT
dc.subjectAnimalspt_PT
dc.subjectAntibodies, Bacterialpt_PT
dc.subjectBCG Vaccinept_PT
dc.subjectChitosanpt_PT
dc.subjectFemalept_PT
dc.subjectHumanspt_PT
dc.subjectImmunoglobulin Apt_PT
dc.subjectImmunoglobulin Gpt_PT
dc.subjectMacrophagespt_PT
dc.subjectMicept_PT
dc.subjectMice, Inbred BALB Cpt_PT
dc.subjectMycobacterium bovispt_PT
dc.subjectTHP-1 Cellspt_PT
dc.titleBCG-loaded chitosan microparticles: interaction with macrophages and preliminary in vivo studiespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FDTP%2F04138%2F2013/PT
oaire.citation.endPage217pt_PT
oaire.citation.issue2pt_PT
oaire.citation.startPage203pt_PT
oaire.citation.titleJournal of Microencapsulationpt_PT
oaire.citation.volume34pt_PT
oaire.fundingStream5876
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublicationd9385154-e0b1-408f-ab26-86ea9ee5ccaa
relation.isProjectOfPublication.latestForDiscoveryd9385154-e0b1-408f-ab26-86ea9ee5ccaa

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