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Orientador(es)
Resumo(s)
The aim of this study was to develop a novel BCG-loaded chitosan vaccine with high association efficiency which can afford efficient interaction with APC and elicit local and Th1-type-specific immune response after intranasal administration. Chitosan-suspended BCG and BCG-loaded chitosan-alginate microparticles were prepared by ionotropic gelation. Interaction with APC was evaluated by fluorescence microscopy using rBCG-GFP. Specific immune responses were evaluated following intranasal immunization of mice. Cellular uptake was approximately two-fold higher for chitosan-suspended BCG. A single dose of BCG-loaded microparticles or chitosan-suspended BCG by intranasal route improved Th1-type response compared with subcutaneous BCG. Chitosan-suspended BCG originated the highest mucosal response in the lungs by the intranasal route. These positive results indicate that the proposed approach of whole live BCG microencapsulation in chitosan-alginate for intranasal immunization was successful in allowing efficient interaction with APC while improving the cellular immune response, which is of interest for local immunization against tuberculosis.
Descrição
Palavras-chave
Administration, Intranasal Animals Antibodies, Bacterial BCG Vaccine Chitosan Female Humans Immunoglobulin A Immunoglobulin G Macrophages Mice Mice, Inbred BALB C Mycobacterium bovis THP-1 Cells
Contexto Educativo
Citação
Caetano LA, Figueiredo L, Almeida AJ, Gonçalves LM. BCG-loaded chitosan microparticles: interaction with macrophages and preliminary in vivo studies. J Microencapsul. 2017;24(2):203-17.
Editora
Taylor & Francis