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Internship: exposure assessment of microbial contamination in different indoor environments

2024Master thesis Open access
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datacite.subject.sdg03:Saúde de Qualidade
dc.contributor.advisorViegas, Carla
dc.contributor.authorSousa, Margarida Rodriguez
dc.date.accessioned2025-02-12T11:38:50Z
dc.date.available2025-02-12T11:38:50Z
dc.date.issued2024
dc.descriptionMestrado em Tecnologias Clínico-Laboratoriais
dc.description.abstractABSTRACT: This internship report is part of the Project/Thesis/Internship course in the 2nd year of the Master's program in Clinical Laboratory Technologies at the Lisbon School of Health Technology (ESTeSL). The professional internship took place at ESTeSL's Environmental Health Laboratory from September 2023 to May 2024, focusing on Public Health and Occupational Exposure. During the internship, sampling campaigns were conducted in primary schools and sawmills to evaluate microbiological contamination (bacteria and fungi) by collecting bioaerosols, using active and passive methods. In the laboratory, activities encompassed classical microbiology (cultureomics) and molecular techniques such as qPCR and DNA extraction. Additionally, an SOP was developed for screening azoles in Aspergillus section Fumigati isolates to define the resistance profile, and a systematic review on WWTP was authored, alongside participation in a congress. These tasks facilitated the consolidation of theoretical knowledge from the 1st year of the Master's program and the acquisition of competencies in Occupational Health, Microbiology, and Molecular Techniques, achieving the programmed objectives. This enriching experience also provided valuable skills in research and the daily life of a researcher, contributing significantly to future professional development.eng
dc.description.abstractEste relatório de estágio faz parte do curso Projeto/Tese/Estágio do 2º ano do Mestrado em Tecnologias Clínico-Laboratoriais na Escola Superior de Tecnologia da Saúde de Lisboa (ESTeSL). O estágio profissional ocorreu no Laboratório de Saúde Ambiental da ESTeSL de setembro de 2023 a maio de 2024, com foco em Saúde Pública e Exposição Ocupacional. Durante o estágio, foram realizadas campanhas de amostragem em escolas primárias e carpintarias para avaliar a contaminação microbiológica (bactérias e fungos) através da colheita bioaressóis, através de métodos ativos e passivos. No laboratório, foram realizadas atividades de microbiologia clássica (culturoômica) e técnicas moleculares como qPCR e extração de DNA. Além disso, foi desenvolvida uma SOP para o screening de azóis em isolados de Aspergillus seção Fumigati para definir o perfil de resistência, e foi escrita uma revisão sistemática sobre WWTP, além da participação em um congresso. Essas atividades permitiram a consolidação dos conhecimentos teóricos adquiridos no 1º ano do programa de mestrado e a aquisição de competências nas áreas de Saúde Ocupacional, Microbiologia e Técnicas Moleculares, cumprindo os objetivos estabelecidos. Esta experiência enriquecedora também proporcionou adquirir capacidades valiosas em investigação e no cotidiano de um investigador, contribuindo significativamente para o desenvolvimento profissional futuro.por
dc.description.sponsorshipI gratefully acknowledge the FCT/MCTES national support through the UIDB/05608/2020; and UIDP/05608/2020. This work is also supported by national funds through FCT/MCTES/FSE/UE, 2023.01366.BD; UI/BD/153746/2022 and CE3C unit UIDB/00329/2020; UI/BD/151431/2021; and Instituto Politécnico de Lisboa, national support through IPL/2022/InChildhealth/BI/12M; IPL/IDI&CA2023/FoodAIIEU_ESTeSL; IPL/IDI&CA2023/ASPRisk_ESTeSL; IPL/IDI&CA2023/ ARAFSawmills_ESTeSL. Partly funded by the European Union. Views and opinions expressed are however those of the author(s) only and do not necessarily reflect those of the European Union or the Swiss State Secretariat for Education, Research and Innovation (SERI), or the United Kingdom Research and Innovation (UKRI), or the Australian National Health & Medical Research Council (NHMRC). Neither the European Union nor the granting authorities can be held responsible for them.
dc.identifier.citationSousa MR. Internship: exposure assessment of microbial contamination in different indoor environments [dissertation]. Lisboa: Escola Superior de Tecnologia da Saúde de Lisboa/Instituto Politécnico de Lisboa; 2024.
dc.identifier.tid203738721
dc.identifier.urihttp://hdl.handle.net/10400.21/21430
dc.language.isoeng
dc.peerreviewedyes
dc.publisherInstituto Politécnico de Lisboa
dc.relationIPL/2022/InChildhealth/BI/12M
dc.relationIPL/IDI&CA2023/FoodAIIEU_ESTeSL
dc.relationIPL/IDI&CA2023/ASPRisk_ESTeSL
dc.relationIPL/IDI&CA2023/ ARAFSawmills_ESTeSL
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectOccupational exposure
dc.subjectMicrobiology
dc.subjectAspergillus section Fumigati
dc.subjectBioaerosols
dc.subjectExposição ocupacional
dc.subjectMicrobiologia
dc.subjectAspergillus secção Fumigati
dc.subjectBioaerossóis
dc.subjectMTCL
dc.subjectFCT_UIDB/05608/2020
dc.subjectFCT_UIDP/05608/2020
dc.subjectIPL/2022/InChildhealth/BI/12M
dc.subjectIPL/IDI&CA2023/FoodAIIEU_ESTeSL
dc.subjectPL/IDI&CA2023/ASPRisk_ESTeSL
dc.subjectIPL/IDI&CA2023/ ARAFSawmills_ESTeSL
dc.titleInternship: exposure assessment of microbial contamination in different indoor environmentseng
dc.typemaster thesis
dspace.entity.typePublication
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85

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