Percorrer por autor "Van-Dúnem, Pedro José Dias"
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- Microscopic and biomolecular result of survey filarias diagnostic in the community of Angola in Chicala province of BiéPublication . Van-Dúnem, Pedro José Dias; lamezon, Sandra Lopez; Brito, Miguel; Lemos, Manuel; Catumbela, Silvano; Pinda, Salas; Capusso, Argílio; Braga, Ginga Luciano; Brás, João; Figueiredo, Jacinta ChavesBié is referred to as an endemic location for filariasis and has never been selected for mass administration of ivermectin. To understand the situation, we did a study in the locality of Chicala, in Kuito, and evaluated the co-endemicity of human filariasis, to obtain data via calibrated thick droplet microscopy and subcutaneous biopsy of samples collected in Chicala in Kuito, Bié and samples collected and preserved on Wathman filter paper for molecular diagnosis of filariae; Material and methods; The research was approved by the ethics committee of the Agostinho Neto University medical school. in deliberation number: 17/2021 and the samples collected after free and informed consent, the universe was 6015 people, data obtained from the local administration and was selected probabilistically from an approximate value of n0 400, the sample of 320 participants distributed in 12 conglomerates where we did research prolectively; The field work involved 2 microscopes, to read the slides, 2 interviewers, 1 guide and 1 driver; The Oncocerca volvulus worm was isolated from subcutaneous cellular tissue extracted from the iliac crest and/or calf muscles and crushed on a glass slide, hydrated in 1 drop of saline and analyzed using a binocular microscope (HC Olympus®), with a 100x objective., regarding the presence or absence of the filaria Oncocerca volvulus and one drop of fresh blood was collected by digital puncture and applied to the slide in a smear and on top of the slide and stained with giemsa and analyzed under the Binocular microscope (HC Olympus®) for the presence or absence of the filariae Wuchereria bancrofty and Loa Loa. All survey data were entered into Microsoft Excel 2010 and IBM SPSS version 21 statistical software was used for analysis. Results The average age of participants in the study was 39.8 years, the findings found in the calibrated thick drop were 0.3%, (+) positive for Wuchereria Bancrofty and subcutaneous biopsy 52.8% (+) positive for Oncocerca volvulus, 169/320 tests, 62.8% of participants were female, 0.3% of male participants had a positive calibrated thick drop (+) for Wuchereria Bancrofty, no observation of the characteristic eye worm was not registered Loa loa and 35% of women had a positive biopsy (+) and 17% of men had a biopsy (+) for Oncocerca volvulus at the age of 46-61 years, 0.3% had a positive (+) for Wuchereria Bancrofty and, 17.5% positive (+) for Oncocerca volvulus, and from 14 -29 years old, 17.2%, (+) and 30-45 years old, 14.1% for Oncocerca volvulus. The results in the conglomerates were: Ngombakasi, 0.3% positive (+) for Wuchereria Bancrofty and Chilomba 8.1%, Lumbachagi, 7.2%, Kalale, 6.9%, and João Kapa 6.6% positive (+), for Oncocerca volvulus, the results of samples preserved on filter paper from the residents of Chikala are missing. Conclusions. The Chicala in the municipality of Kuito, province of Bié, is hyperendemic for Oncocerca volvulus, a prevalence above 50%, and hypoendemic for Wuchereria Bancrofty, a prevalence lower than 10% with Loiasis outbreaks in the province to be determined; which allows the provincial authorities to invest in the mass administration of ivermectin because the risk of side effects is minimal or irrelevant.
- Socio-environmental factors associated with knowledge, attitude and practice and determinants of co-endemicity of filariasis in Chicala, Kuito province of Bié, AngolaPublication . Van-Dúnem, Pedro José Dias; lamezon, Sandra Lopez; Brito, Miguel; Lemos, Manuel; Figueiredo, Jacinta ChavesFilariasis is an acute and chronic parasitic disease, caused by filaria of the species: Oncocerca Volvulos, Wuchereia bancrofty and Loíase, whose injuries have serious consequences for human health and as it is endemic in Angola, its study and control become imperative. Objective: To carry out an integrated study to identify filarial co-infection associated with social determinants and knowledge, attitude, and practice of the population of Chicala, commune of Kuíto, province of Bié. Material and Method: After prior contact with administrators, a cross-sectional lecture study was carried out and a probabilistic cluster sample of 320 individuals from a universe of 6015 individuals was selected. After free and informed consent, a questionnaire was administered to collect clinical-socio-demographic data: Leopard skin sign or depigmentation, Blindness, elephant's foot, hydrocele, and presence of eye worm. A biopsy of subcutaneous cellular tissue taken from the iliac crest or calf muscles was performed and a calibrated thick drop (GEC) was performed after digital puncture and a drop of dried blood was preserved on filter paper strips for the genetic detection of the species. Included photos with vector image Simulium, Crysops and Anopheles, Aedes, and Culex to help interviewees inform about the diseases under study. Positive tests referred to the provincial NTD program to take Ivermetin 6mg/dose, each participant received one albendazole 400 mg tablet. For data processing, the Chi-square test was applied with a significance level of 0.05. Results: The average age of the participants was 32.5 years and the female sex was more represented 200 (62.5%) the male 120 (37.5%), the significance level was higher than 0.05, therefore the variables are not associated, so it is not pertinent to apply logarithmic analysis to associate knowledge, attitude, and practice with the inequities observed in social determinants, through a logarithmic linear model that allows detecting more significant effects and interpreting the relationships between the variables. The macroscopic lesions found were: (5.9%) hydrocele, 13(68.4%) Elephantiasis 3(15.7%) calibrated thick drop, 1(0.3%) positive for Wuchereia bancrofty and (0) for Loiase; Leopard skin (depigmentation),8(2.5%) Blindness 3(15,7%), biopsy 174(%54.3) positive for Oncocerca Volvulos; The Knowledge, Attitude, and Practice of the population the result was: Knowledge: (100%) inadequate; Attitude: (17%) marginalizes hydrocele, (29%) marginalizes blindness, (34%) marginalizes hydrocele, blindness and elephantiasis; Practice: (99.4%) uses repellent or sleeved shirt to protect against vector bites and 100% have never attended a lecture on filariae (99.7%) do not sleep under a mosquito net and (99.7%) do not take albendazole. Conclusions: The clinical-laboratory findings prove the existence of co-infection: Oncocerca Volvulos and Wuchereria bancrofty in the adult population and stimulate the distribution of ivermectin for prevention and treatment in Chicala under Community Directive without risk of side effects after the molecular biology results confirm the GEC findings, we recommend informing the population about filariasis associated with the distribution of ivermectin and the improvement of social determinants in chicala.