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Percorrer por autor "Gouveia, Ana Filipa"

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  • The need of a one health approach to tackle microbiological contamination in animal and dairy production: the case of Portuguese feedlots farms and dairies
    Publication . Viegas, Carla; Gouveia, Ana Filipa; Cervantes, Renata; Pena, Pedro; Carolino, Elisabete; Twarużek, Magdalena; Viegas, Susana
    With the global population rising, there is increasing demand for agricultural productivity, particularly for animal-derived proteins and products. This trend places additional pressure on natural resources and the workforce in the animal production sector. This study aims to address environmental and health factors influenced by animal production within the One Health framework, encompassing animal welfare, food security, food safety, workers' health, and climate change. In this context, three feedlot farms and two dairies from the North of Portugal, representing the worst-case exposure scenario, were engaged in this study. Samples were collected using Electrostatic Dust Cloths (EDC) placed on farm surfaces and attached to work clothing (EDCT). Additional samples were obtained from feed, used bedding material, and surface swabs from feeders, drinkers, milking parlors, and frequently touched areas in social spaces (e.g., offices and changing rooms). Microbial characterization and azole-resistance screening were performed using diverse culture media, complemented by molecular assays (qPCR) targeting toxigenic fungal species. Thirty-eight mycotoxins were analyzed across the sampled matrices. This comprehensive approach identified critical sources of microbial and mycotoxin contamination: bedding material showed the highest bacterial contamination (TSA; 5.40 X 103 CFU.g-1), while swabs (MEA; 2.5 X 104 CFU.m-2 to 9.00 X 104 CFU.m-2) and feed (MEA; ranged from 1.33 X 102 CFU.g-1 to 8.00 X 102 CFU.g-1) exhibited the greatest fungal contamination. Feed was identified as the main source of mycotoxin exposure for both animals and workers, since all 16 feed samples tested positive for mycotoxin contamination. Results revealed widespread distribution of Aspergillus sp. across environmental matrices, highlighting the need for targeted interventions. Azole-resistance screening and mycotoxin profiling further emphasize the importance of implementing targeted interventions to prevent, monitor, and remediate environmental contamination by fungi and mycotoxins across different contexts (food safety, animal health, public and occupational health), underscoring the value of a One Health approach.
    2026-05Artigo científico Acesso aberto Ver mais
  • One Health approach in feedlots and dairies
    Publication . Viegas, Carla; Gouveia, Ana Filipa; Cervantes, Renata; Pena, Pedro; Carolino, Elisabete; Twaruzek, Magdalena; Viegas, Susana
    Growing demand for animal products increases pressure on agriculture; workers are exposed to bacteria, fungi, and toxins in livestock farms; climate change increases fungal adaptation and mycotoxin risks; and One Health links animal, human, and environmental health. This study aimed to assess microbial contamination in feedlots and dairies; evaluate azole-resistant fungi and mycotoxins; and apply a One Health framework.
    2026-06Comunicação em conferência Acesso aberto Ver mais