Percorrer por autor "Alves, Ana"
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- Dry blood spots in filter paper: a valuable option for DNA extraction?Publication . Alves, Ana; Xisto, Ana; Tomaz, Beatriz; Brito, Miguel; Ribeiro, EdnaABSTRACT - Dry blood spots, appear to be a valuable option for sample collection for DNA extraction, especially in poorer communities with less access to medical care. Dry blood spots can be easily transported and ensure a non-invasive method of rapid blood collection which can be coupled with DNA extraction methods. However, the storage and transportation conditions (time and temperature) as well as sample type and volumes may affect DNA extraction efficiency and become a challenge. In this context, we aim to assess the effects on DNA extraction efficiency of six storage conditions (24 hours at 4 °C, 24 hours at room temperature, 24 hours at 50 °C, seven days at 4 °C, seven days at room temperature, and seven days at 50 °C) for dry blood spots with venous blood and capillary blood samples. To evaluate DNA extraction proficiency, under each of these conditions, we performed DNA extraction with the QIAamp® Blood Mini Kit and assessed DNA final concentrations and purity ratios, through UV spectrophotometry, with the aid of the Nanodrop® OneC. Statistical analysis was performed using a T-test in Microsoft® Excel. Overall, our data demonstrated that it is possible to extract a greater DNA quantity from capillary blood samples in dry blood spots, although with less purity than venous blood samples. Also, our results show that the assessed temperature and storage time (seven days) do not affect DNA extraction results.
- National recommendations for the safe handling of hazardous drugs by pharmacy technicians in Portugal: a modified delphi studyPublication . Silva, Vítor; Valeiro, Carolina; Alves, Ana; Costa, Ana; Costa-Veiga, Ana; Reis, Ana Rita; Simões, Ana Rita; Silva, Ana; Andrade, Anabela; Oliveira, Andreia; Marques, André; Perpétuo, Carla; Pereira, Cátia; Carriço, Cristina; Vieira, Elisabete; Moreira, fernando; Santos, Joana; Vilar, Joana; Joaquim, João; Valadares, Luís; Baeta, Maria; Couras, Mariana; Ramires, Milene; Silva, Nuno Miguel; Pereira, Olívia R.; Guerra, Paula Bustorff; Alexandre, Pedro; Pinto, Rafaela; Pelota, Ricardo; Gomes, Sofia Alves; Grilo, Sofia Roque; Vieira, Sofia; Sequeira, Susana; Guedelha, Tânia; Freire, Tiago; Oliveira, Vânia; Matos, CristianoBackground: Occupational exposure to hazardous drugs is a recognised occupational risk in hospital pharmacy practice. Although international guidelines are available, the implementation is heterogeneous, and there are currently no formally endorsed national recommendations regarding the safety of pharmacy technicians in Portugal. Objective: To develop consensus-based national recommendations for the safe handling of hazardous drugs by pharmacy technicians in Portugal. Methods: A modified Delphi study was conducted involving 43 invited experts from Portuguese hospital pharmacies. Fifty-eight statements were evaluated using a 0-10 agreement scale through one formal quantitative round, followed by a structured online consensus validation meeting and documentary confirmation. Consensus was predefined as a median score >7. Results: Thirty-six experts completed Round 1 (response rate 83.7%). All 58 statements voted on in Round 1 achieved consensus, with median scores of 10 for all recommendations and 98% of ratings ≥7. Following consolidation and merging procedures, the final set comprised 55 recommendations. The final recommendations were organised into five domains: occupational health surveillance, engineering controls and PPE, preparation technique and quality assurance, administrative safeguards, and environmental contamination and waste management. Conclusions: This study establishes the first structured national consensus on the safety of hazardous drug handling for pharmacy technicians in Portugal. The resulting framework supports harmonisation of institutional practices, strengthens occupational risk governance, and provides a foundation for future implementation and evaluation studies.