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HIV-1 diversity and pre-treatment drug resistance in the era of integrase inhibitor among newly diagnosed ART-naïve adult patients in Luanda, Angola

2024-07Journal article Open access
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dc.contributor.authorSebastião, Cruz S.
dc.contributor.authorAbecasis, Ana B.
dc.contributor.authorJandondo, Domingos
dc.contributor.authorSebastião, Joana M.
dc.contributor.authorVigário, João
dc.contributor.authorComandante, Felícia
dc.contributor.authorPingarilho, Marta
dc.contributor.authorPocongo, Bárbara
dc.contributor.authorCassinela, Edson
dc.contributor.authorGonçalves, Fátima
dc.contributor.authorGomes, Perpétua
dc.contributor.authorGiovanetti, Marta
dc.contributor.authorFrancisco, Ngiambudulu M.
dc.contributor.authorSacomboio, Euclides
dc.contributor.authorBrito, Miguel
dc.contributor.authorNeto de Vasconcelos, Jocelyne
dc.contributor.authorMorais, Joana
dc.contributor.authorPimentel, Victor
dc.date.accessioned2024-07-24T09:19:29Z
dc.date.available2024-07-24T09:19:29Z
dc.date.issued2024-07
dc.descriptionThis research was funded by the Fundação Calouste Gulbenkian (FCG), under the ENVOLVE Ciência PALOP program that funded the HITOLA project (Number 250466), AREF (AREF-312-CRUZ-F-C0931), Science and Technology Development Project (PDCT) within the scope of the MUTHIVAO project (Number 36 MESCTI/PDCT/2022), FCT MARVEL (PTDC/SAU-PUB/4018/2021), FCT GHTM-UID/04413/2020 and LA-REAL-LA/P/0117/2020.pt_PT
dc.description.abstractThe surveillance of drug resistance in the HIV-1 naïve population remains critical to optimizing the effectiveness of antiretroviral therapy (ART), mainly in the era of integrase strand transfer inhibitor (INSTI) regimens. Currently, there is no data regarding resistance to INSTI in Angola since Dolutegravir-DTG was included in the first-line ART regimen. Herein, we investigated the HIV-1 genetic diversity and pretreatment drug resistance (PDR) profile against nucleoside/tide reverse transcriptase inhibitors (NRTIs), non-nucleoside reverse transcriptase inhibitors (NNRTIs), protease inhibitors (PIs), and INSTIs, using a next-generation sequencing (NGS) approach with MinION, established to track and survey DRMs in Angola. This was a cross-sectional study comprising 48 newly HIV-diagnosed patients from Luanda, Angola, screened between March 2022 and May 2023. PR, RT, and IN fragments were sequenced for drug resistance and molecular transmission cluster analysis. A total of 45 out of the 48 plasma samples were successfully sequenced. Of these, 10/45 (22.2%) presented PDR to PIs/NRTIs/NNRTIs. Major mutations for NRTIs (2.2%), NNRTIs (20%), PIs (2.2%), and accessory mutations against INSTIs (13.3%) were detected. No major mutations against INSTIs were detected. M41L (2%) and I85V (2%) mutations were detected for NRTI and PI, respectively. K103N (7%), Y181C (7%), and K101E (7%) mutations were frequently observed in NNRTI. The L74M (9%) accessory mutation was frequently observed in the INSTI class. HIV-1 pure subtypes C (33%), F1 (17%), G (15%), A1 (10%), H (6%), and D (4%), CRF01_AG (4%) were observed, while about 10% were recombinant strains. About 31% of detected HIV-1C sequences were in clusters, suggesting small-scale local transmission chains. No major mutations against integrase inhibitors were detected, supporting the continued use of INSTI in the country. Further studies assessing the HIV-1 epidemiology in the era of INSTI-based ART regimens are needed in Angola.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSebastião CS, Abecasis AB, Jandondo D, Sebastião JM, Vigário J, Brito M, et al. HIV-1 diversity and pre-treatment drug resistance in the era of integrase inhibitor among newly diagnosed ART-naïve adult patients in Luanda, Angola. Sci Rep. 2024;14(1):15893.pt_PT
dc.identifier.doi10.1038/s41598-024-66905-1pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/17582
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherNaturept_PT
dc.relationGHTM-UID/04413/2020pt_PT
dc.relationLA-REAL-LA/P/0117/2020pt_PT
dc.relationAREF-312-CRUZ-F-C0931pt_PT
dc.relationMESCTI/PDCT/2022pt_PT
dc.relation.publisherversionhttps://www.nature.com/articles/s41598-024-66905-1pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectGeneticspt_PT
dc.subjectDrug resistance mutationspt_PT
dc.subjectGenetic diversitypt_PT
dc.subjectHIV-1pt_PT
dc.subjectINSTIpt_PT
dc.subjectIntegrase strand transfer inhibitorpt_PT
dc.subjectNGSpt_PT
dc.subjectNext-generation sequencingpt_PT
dc.subjectAngolapt_PT
dc.subjectLuandapt_PT
dc.subjectPTDC/SAU-PUB/4018/2021pt_PT
dc.subjectGHTM-UID/04413/2020pt_PT
dc.subjectLA-REAL-LA/P/0117/2020pt_PT
dc.subjectAREF-312-CRUZ-F-C0931pt_PT
dc.subjectMESCTI/PDCT/2022pt_PT
dc.titleHIV-1 diversity and pre-treatment drug resistance in the era of integrase inhibitor among newly diagnosed ART-naïve adult patients in Luanda, Angolapt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-PUB%2F4018%2F2021/PT
oaire.citation.issue1pt_PT
oaire.citation.startPage15893pt_PT
oaire.citation.titleScientific Reportspt_PT
oaire.citation.volume14pt_PT
oaire.fundingStream3599-PPCDT
person.familyNameBrito
person.givenNameMiguel
person.identifier.ciencia-id231F-F341-7E93
person.identifier.orcid0000-0001-6394-658X
person.identifier.ridA-7970-2016
person.identifier.scopus-author-id35224551000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication4252d8e0-800c-4d67-8b13-0b711d860669
relation.isAuthorOfPublication.latestForDiscovery4252d8e0-800c-4d67-8b13-0b711d860669
relation.isProjectOfPublication2a475e9e-7717-4ca3-8d16-1906bbf12676
relation.isProjectOfPublication.latestForDiscovery2a475e9e-7717-4ca3-8d16-1906bbf12676

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