| Name: | Description: | Size: | Format: | |
|---|---|---|---|---|
| 3.87 MB | Adobe PDF |
Authors
Advisor(s)
Abstract(s)
Este projeto baseia-se no estudo das propriedades protetoras de barreiras microbianas eletricamente ativas para aplicação em queimados. As queimaduras representam um problema de particular relevância, pois muitos pacientes enfrentam grandes dificuldades relacionadas com alterações físicas, em termos de aparência e funcionalidade, o que tem grande impacto a nível psicológico. Com este trabalho, pretendeu-se avaliar a conceção e construção de barreiras à entrada de microrganismos e outros agentes patogénicos através de feridas, na forma de pensos eletroativos. A intervenção destes pensos tem como finalidade o tratamento do doente queimado, a prevenção de sequelas e redução do tempo de internamento, visando a restauração e funcionalidade da área queimada. Inicialmente, procedeu-se à impressão de pensos na forma de circuitos elétricos sobre vários tipos de substratos, utilizando um polímero orgânico condutor conhecido como PEDOT:PSS, como tinta, e uma impressora de jato de tinta de elevada resolução - Dimatix Fujifilm. Posteriormente foi medida a resistência elétrica dos mesmos para avaliar a efetividade da impressão nos diferentes substratos utilizados, nomeadamente em papel de filtro e em papel de impressão. Avaliou-se a combinação das características “cobertura de tinta” obtida na impressão e a intensidade de corrente medida para planear um circuito de desenho otimizado, com dimensões adequadas à sua colocação em caixa de petri, para futura inoculação de microrganismos. Com este novo circuito, foram realizadas impressões com tinta de prata. Incubaram-se os circuitos em vários tipos de substratos – papel de impressão e papel de filtro – utilizando quer a tinta PEDOT:PSS quer a tinta de prata, e os mesmos foram inoculados com água residual tratada, num meio de Luria Bertani e com imposição de corrente. Observou-se a evolução do desenvolvimento dos microrganismos presentes na água, a uma temperatura de incubação de 37°C, sobre os circuitos impressos, sob a aplicação de um campo elétrico aos mesmos e na sua ausência.
ABSTRACT - This project is based on the study of the protective properties of electrically active microbial barriers for application in patients with burns. Burns is undoubtedly a very sensitive issue, many patients face major difficulties related to physical changes in terms of appearance and functionality, which has a great psychological impact. With this work, we intend to evaluate the design and construction of barriers to act against the entry of micro-organisms and other pathogens through wounds, in the form of electroactive dressings. The intervention of these dressings aims to treat the burn patient, prevent sequels and reduce the length of stay in the hospital, aiming at the restoration and functionality of the burned area. Initially, PEDOT:PSS ink circuits were printed to optimize and understand the operation of the Dimatix Fujifilm printer used for printing these circuits. Subsequently, current measurements were made to the printed circuits to assess how they behaved in the different substrates used, namely filter paper and printing paper. The cover obtained in the printout and the amount of current obtained were studied to then create a new circuit design that would allow it to be placed in a Petri box to allow inoculation of microorganisms. With this new circuit, silver ink prints were made, and this in itself has already presented antimicrobial properties. The printed circuits were incubated on the different substrates and with the different inks, inoculated with wastewater treated in a Luria Bertani medium, and having a current passed through them. The behavior of the microorganisms present in the water was observed when incubated at 37°C in printed circuits to assess which circuit is most advantageous for the design of the electroactive dressings.
ABSTRACT - This project is based on the study of the protective properties of electrically active microbial barriers for application in patients with burns. Burns is undoubtedly a very sensitive issue, many patients face major difficulties related to physical changes in terms of appearance and functionality, which has a great psychological impact. With this work, we intend to evaluate the design and construction of barriers to act against the entry of micro-organisms and other pathogens through wounds, in the form of electroactive dressings. The intervention of these dressings aims to treat the burn patient, prevent sequels and reduce the length of stay in the hospital, aiming at the restoration and functionality of the burned area. Initially, PEDOT:PSS ink circuits were printed to optimize and understand the operation of the Dimatix Fujifilm printer used for printing these circuits. Subsequently, current measurements were made to the printed circuits to assess how they behaved in the different substrates used, namely filter paper and printing paper. The cover obtained in the printout and the amount of current obtained were studied to then create a new circuit design that would allow it to be placed in a Petri box to allow inoculation of microorganisms. With this new circuit, silver ink prints were made, and this in itself has already presented antimicrobial properties. The printed circuits were incubated on the different substrates and with the different inks, inoculated with wastewater treated in a Luria Bertani medium, and having a current passed through them. The behavior of the microorganisms present in the water was observed when incubated at 37°C in printed circuits to assess which circuit is most advantageous for the design of the electroactive dressings.
Description
Mestrado em Engenharia Biomédica
Keywords
Queimadura Circuito Penso eletroativo Impressão por jato de tinta Meio Luria Bertani Burn Circuit Electroactive dressing Inkjet printing Luria Bertani medium
Citation
Marques CC. Estudo das propriedades protetoras de barreiras microbianas elétricamente ativas para aplicação em queimados [dissertation]. Lisboa: Escola Superior de Tecnologia da Saúde de Lisboa - Instituto Superior de Engenharia de Lisboa/Instituto Politécnico de Lisboa; 2019.
Publisher
Instituto Politécnico de Lisboa, Escola Superior de Tecnologia da Saúde de Lisboa