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GASMoC method: a phenol-free technique to detect acid-fast bacilli

2020-01Journal article Open access
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dc.contributor.authorGomes, A.
dc.contributor.authorAmaral, P.
dc.contributor.authorSantos, R.
dc.contributor.authorSantos, S.
dc.contributor.authorTortosa, F.
dc.contributor.authorMendonça, Paula
dc.contributor.authorMarques-Ramos, Ana
dc.date.accessioned2019-11-14T17:57:25Z
dc.date.available2019-11-14T17:57:25Z
dc.date.issued2020-01
dc.description.abstractThe genre Mycobacterium includes a series of pathological species, such as M. tuberculosis, which is important for pathology laboratories to detect for a correct diagnosis. The Ziehl-Neelsen technique (ZNT) is the most common histochemical method used to detect these bacilli and uses a heated mixture of carbol-fuchsine, which contains basic fuchsine and phenol. Whereas the former component is responsible for the pinkish staining of acid-fast mycobacteria, the role of phenol is not completely understood and it has been suggested that its exclusion does not impact the detection ability of the ZNT. Since phenol is highly toxic and induces several injuries, the goal of this study is to determine the detection capacity of mycobacteria through a method that excludes the use of phenol. Accordingly, the GASMoC method, a modified ZNT that employs a solution of aqueous basic fuchsine heated at 37 °C, was tested on histological samples positive for mycobacteria and the results were compared to that of the ZNT. Data demonstrated that the GASMoC method was able to detect acid-fast bacilli (AFB) in all analyzed cases. Remarkably, microscopic inspection of mycobacteria was easier when the GASMoC method was applied. In conclusion, our study demonstrates that AFB detection does not require phenol and that the GASMoC method, a phenol-free technique, may substitute the ZNT in pathology laboratories.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationGomes A, Amaral P, Santos R, Santos S, Mendonça P, Marques-Ramos A, et al. GASMoC method: a phenol-free technique to detect acid-fast bacilli. Histochem Cell Biol. 2020;153:63-9.pt_PT
dc.identifier.doi10.1007/s00418-019-01825-0pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/10709
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.relation.publisherversionhttps://link.springer.com/article/10.1007%2Fs00418-019-01825-0pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectAqueous basic fuchsinept_PT
dc.subjectCarbol-fuchsinept_PT
dc.subjectModified Ziehl-Neelsen techniquept_PT
dc.subjectMycobacteria detectionpt_PT
dc.subjectMycobacterium tuberculosispt_PT
dc.subjectSafe acid-fast bacilli detectionpt_PT
dc.titleGASMoC method: a phenol-free technique to detect acid-fast bacillipt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage69pt_PT
oaire.citation.startPage63pt_PT
oaire.citation.titleHistochemistry and Cell Biologypt_PT
oaire.citation.volume153pt_PT
person.familyNameMarques-Ramos
person.givenNameAna
person.identifier.ciencia-id0617-3550-881E
person.identifier.orcid0000-0003-2655-8806
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication0b8d516d-bf23-4db0-8224-d33e28d309ac
relation.isAuthorOfPublication.latestForDiscovery0b8d516d-bf23-4db0-8224-d33e28d309ac

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