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A high throughput colorimetric assay of beta-1,3-D-glucans by Congo red dye

2015-02Journal article Restricted access
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dc.contributor.authorSemedo, Madga Sofia Cardoso Nobre
dc.contributor.authorKarmali, Amin
dc.contributor.authorFonseca, Luís
dc.date.accessioned2016-03-09T12:42:42Z
dc.date.available2016-03-09T12:42:42Z
dc.date.issued2015-02
dc.description.abstractMushroom strains contain complex nutritional biomolecules with a wide spectrum of therapeutic and prophylactic properties. Among these compounds, β-d-glucans play an important role in immuno-modulating and anti-tumor activities. The present work involves a novel colorimetric assay method for β-1,3-d-glucans with a triple helix tertiary structure by using Congo red. The specific interaction that occurs between Congo red and β-1,3-d-glucan was detected by bathochromic shift from 488 to 516 nm (> 20 nm) in UV–Vis spectrophotometer. A micro- and high throughput method based on a 96-well microtiter plate was devised which presents several advantages over the published methods since it requires only 1.51 μg of polysaccharides in samples, greater sensitivity, speed, assay of many samples and very cheap. β-d-Glucans of several mushrooms (i.e., Coriolus versicolor, Ganoderma lucidum, Pleurotus ostreatus, Ganoderma carnosum, Hericium erinaceus, Lentinula edodes, Inonotus obliquus, Auricularia auricular, Polyporus umbellatus, Cordyseps sinensis, Agaricus blazei, Poria cocos) were isolated by using a sequence of several extractions with cold and boiling water, acidic and alkaline conditions and quantified by this microtiter plate method. FTIR spectroscopy was used to study the structural features of β-1,3-d-glucans in these mushroom samples as well as the specific interaction of these polysaccharides with Congo red. The effect of NaOH on triple helix conformation of β-1,3-d-glucans was investigated in several mushroom species.pt_PT
dc.identifier.citationSEMEDO, M. C.; KARMALI, A.; FONSECA, L. - A high throughput colorimetric assay of beta-1,3-D-glucans by Congo red dye. Journal of Microbiological Methods. ISSN. 0167-7012. Vol. 109 (2015), pp. 140-148pt_PT
dc.identifier.doi10.1016/j.mimet.2014.12.020pt_PT
dc.identifier.issn0167-7012
dc.identifier.issn1872-8359
dc.identifier.urihttp://hdl.handle.net/10400.21/5812
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevier B.V.pt_PT
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0167701214003698pt_PT
dc.subjectCongo red dyept_PT
dc.subjectMushroom strainspt_PT
dc.subjectbeta-1,3-D-Glucanpt_PT
dc.subjectHigh-throughput assaypt_PT
dc.subject96-Well microtiter platept_PT
dc.subjectTriple helix structurept_PT
dc.titleA high throughput colorimetric assay of beta-1,3-D-glucans by Congo red dyept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage148pt_PT
oaire.citation.startPage140pt_PT
oaire.citation.volume109pt_PT
person.familyNameSemedo
person.familyNameKarmali
person.givenNameMagda
person.givenNameAmin
person.identifier1022083
person.identifier.ciencia-id9C11-BD02-C77F
person.identifier.orcid0000-0001-6330-2899
person.identifier.orcid0000-0003-0419-401X
person.identifier.ridE-4787-2011
person.identifier.scopus-author-id57000381900
person.identifier.scopus-author-id6603778216
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication73e398bc-a0e8-420d-81eb-34159398838d
relation.isAuthorOfPublication23975b4b-3234-4139-9675-89ea6f9a2332
relation.isAuthorOfPublication.latestForDiscovery23975b4b-3234-4139-9675-89ea6f9a2332

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