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Medical grade honey as a promising treatment to improve ovarian tissue transplantation

2022-07Journal article Open access
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dc.contributor.authorAzevedo, Ana Rita
dc.contributor.authorPais, Ana Sofia
dc.contributor.authorAlmeida-Santos, Teresa
dc.contributor.authorPires, Virgínia M.
dc.contributor.authorPessa, Pedro
dc.contributor.authorMarques, Carla C.
dc.contributor.authorNolasco, Sofia
dc.contributor.authorCastelo-Branco, Pedro
dc.contributor.authorPrates, José A.
dc.contributor.authorLopes-da-Costa, Luís
dc.contributor.authorLaranjo, Mafalda
dc.contributor.authorBotelho, Maria Filomena
dc.contributor.authorPereira, Rosa M.
dc.contributor.authorPimenta, Jorge M.
dc.date.accessioned2022-08-03T10:39:38Z
dc.date.available2022-08-03T10:39:38Z
dc.date.issued2022-07
dc.description.abstractOvarian tissue cryopreservation is a female fertility preservation technique that presents major challenges for the maintenance of follicular viability after transplantation. The aim of this study was to evaluate and compare the application of L-Mesitran Soft®, a product containing 40% medical grade honey (MGH), with other strategies to improve ovarian grafts’ viability. For this purpose, bovine ovarian tissue was vitrified, warmed, and randomly assigned to culture groups: (1) control, (2) MGH 0.2% in vitro, (3) MGH in vivo (direct application in the xenotransplantation), (4) vascular endothelial growth factor (VEGF 50 ng/mL) and (5) vitamin D (100 Nm), during a 48 h period. A sixth group (6) of fragments was thawed on transplantation day and was not cultured. The tissue was xenotransplanted into immunodeficient (Rowett nude homozygous) ovariectomized rats. Grafts were analyzed 48 h after culture, and 7 and 28 days after transplantation. The tissue was subjected to histological and immunohistochemical analysis. Treatments using MGH showed the highest angiogenic and cell proliferation stimulation, with cellular apoptosis, within a healthy cellular turnover pathway. In conclusion, MGH should be considered a potentially effective and less expensive strategy to improve ovarian tissue transplantation.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAzevedo AR, Pais AS, Almeida-Santos T, Pires VM, Pessa P, Nolasco S, et al. Medical grade honey as a promising treatment to improve ovarian tissue transplantation. Bioengineering. 2022;9(8):357.pt_PT
dc.identifier.doi10.3390/bioengineering9080357pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/14905
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relation.publisherversionhttps://www.mdpi.com/2306-5354/9/8/357pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectXenograftingpt_PT
dc.subjectOvarianpt_PT
dc.subjectVitrificationpt_PT
dc.subjectHoneypt_PT
dc.subjectFertilitypt_PT
dc.subjectCryopreservationpt_PT
dc.subjectTransplantationpt_PT
dc.titleMedical grade honey as a promising treatment to improve ovarian tissue transplantationpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue8pt_PT
oaire.citation.startPage357pt_PT
oaire.citation.titleBioengineeringpt_PT
oaire.citation.volume9pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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