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Abstract(s)
A resistência a antimicrobianos é atualmente um dos maiores problemas de saúde pública, sendo necessárias ações que visem combater esta ameaça global. Face a este problema, cada vez mais os compostos derivados de produtos naturais têm sido utilizados como agentes antimicrobianos no desenvolvimento de novos tratamentos para microrganismos resistentes. Face às melhorias tecnológicas os materiais híbridos têm sido altamente investigados, uma vez que as suas caraterísticas promissoras os tornam adequados a diversas aplicações na área da biomédica, podendo ser utilizados como base para novos produtos com propriedades antimicrobianas. O uso de polímeros naturais, que atuam como bases na produção de filmes ou como misturas para a impressão 3D apresenta diversas vantagens de onde se destaca a biocompatibilidade. Adicionalmente estes materiais provêm de fontes renováveis que os tornam muito atrativos em termos de custos e vantagens ambientais. Neste contexto, o presente trabalho teve como objetivo a produção de filmes e peças 3D, fabricados com matrizes poliméricas com base no óleo de soja incorporados com compostos naturais (cinamaldeído, eugenol, o-vanilina e álcool vanílico) a fim de produzir materiais biocompatíveis, com possíveis propriedades antimicrobianas. Outro objetivo foi estudar o perfil de libertação dos compostos dos filmes produzidos. Foram avaliadas as caraterísticas físico-químicas e mecânicas dos filmes incorporados, através da análise de FTIR-ATR, absorção de água e ensaios de tração. De modo a avaliar a atividade antimicrobiana dos compostos selecionados e dos filmes produzidos foi utiliza a técnica de sobreposição de soft-agar, onde foram utilizadas duas estirpes de bactérias, a Staphylococcus aureus e a Escherichia coli.
Na generalidade, os materiais produzidos revelaram-se mais eficazes contra a Staphylococcus aureus e os filmes de resina OSEA mostraram-se os mais promissores. Relativamente aos compostos ativos, a incorporação de cinamaldeído apresentou-se como a mais eficiente para a inibição do crescimento bacteriano.
Antimicrobial resistance is currently one of the biggest concerns regarding public health so there is a crescent need to act against this global threat. Nowadays, more compounds derived from natural products have been used as antimicrobial agents in the development of new treatments against resistant microorganisms. Given technological improvements, hybrid materials have been highly investigated due to their promising characteristics for biomedical applications and can be used as a base for new antimicrobial products. The use of natural polymers, which serve as bases in producing films or mixtures for 3D printing, presents several advantages, especially biocompatibility. Additionally, these materials come from renewable sources making them very attractive in terms of cost and environmental needs. In this context, one of the present work’s goals was to produce films and 3D objects, manufactured with soybean-based resins incorporated with natural compounds (cinnamaldehyde, eugenol, o-vanillin and vanillic alcohol) to produce biocompatible materials with possible antimicrobial properties. Another goal was to study the release model of the compounds dopped in the films. Physicochemical and mechanical properties were determined through FTIR-ATR spectroscopy, water absorption and tensile tests. Furthermore, the antimicrobial activity was investigated, by the Agar diffusion test, against Staphylococcus aureus and Escherichia coli. Generally, the produced materials were more effective against Staphylococcus aureus and the AESO films proved to be the most promising. Regarding the active compounds, the incorporation of cinnamaldehyde was the most efficient in inhibiting bacterial growth.
Antimicrobial resistance is currently one of the biggest concerns regarding public health so there is a crescent need to act against this global threat. Nowadays, more compounds derived from natural products have been used as antimicrobial agents in the development of new treatments against resistant microorganisms. Given technological improvements, hybrid materials have been highly investigated due to their promising characteristics for biomedical applications and can be used as a base for new antimicrobial products. The use of natural polymers, which serve as bases in producing films or mixtures for 3D printing, presents several advantages, especially biocompatibility. Additionally, these materials come from renewable sources making them very attractive in terms of cost and environmental needs. In this context, one of the present work’s goals was to produce films and 3D objects, manufactured with soybean-based resins incorporated with natural compounds (cinnamaldehyde, eugenol, o-vanillin and vanillic alcohol) to produce biocompatible materials with possible antimicrobial properties. Another goal was to study the release model of the compounds dopped in the films. Physicochemical and mechanical properties were determined through FTIR-ATR spectroscopy, water absorption and tensile tests. Furthermore, the antimicrobial activity was investigated, by the Agar diffusion test, against Staphylococcus aureus and Escherichia coli. Generally, the produced materials were more effective against Staphylococcus aureus and the AESO films proved to be the most promising. Regarding the active compounds, the incorporation of cinnamaldehyde was the most efficient in inhibiting bacterial growth.
Description
Trabalho final de Mestrado para obtenção do grau de Mestre em Engenharia Biomédica
Keywords
Atividade antimicrobiana Materiais híbridos Compostos naturais Resina de soja Impressão 3D Antimicrobial activity Hybrid materials Natural compounds Soybean-based resins 3D printing
Citation
AMARAL, Inês Monte Vinha Ferreira - Produção de filmes e peças 3D de materiais híbridos biocompatíveis com potenciais propriedades antimicrobianas. Lisboa: Instituto Superior de Engenharia de Lisboa, 2022. Dissertação de Mestrado.