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Signature cytokine-associated transcriptome analysis of effector γδ T cells identifies subset-specific regulators of peripheral activation

2025-01Journal article Open access
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datacite.subject.sdg03:Saúde de Qualidade
dc.contributor.authorInácio, Daniel
dc.contributor.authorAmado, Tiago
dc.contributor.authorPamplona, Ana
dc.contributor.authorSobral, Daniel
dc.contributor.authorCunha, Carolina
dc.contributor.authorSantos, Rita F.
dc.contributor.authorOliveira, Liliana
dc.contributor.authorRouquié, Nelly
dc.contributor.authorCarmo, Alexandre M.
dc.contributor.authorLesourne, Renaud
dc.contributor.authorGomes, Anita
dc.contributor.authorSilva-Santos, Bruno
dc.date.accessioned2025-02-18T16:09:27Z
dc.date.available2025-02-18T16:09:27Z
dc.date.issued2025-01
dc.description.abstractγδ T cells producing either interleukin-17A (γδ17 cells) or interferon-γ (γδIFN cells) are generated in the mouse thymus, but the molecular regulators of their peripheral functions are not fully characterized. Here we established an Il17a-GFP:Ifng-YFP double-reporter mouse strain to analyze at unprecedented depth the transcriptomes of pure γδ17 cell versus γδIFN cell populations from peripheral lymph nodes. Within a very high fraction of differentially expressed genes, we identify a panel of 20 new signature genes in steady-state γδ17 cells versus γδIFN cells, which we further validate in models of experimental autoimmune encephalomyelitis and cerebral malaria, respectively. Among the signature genes, we show that the co-receptor CD6 and the signaling protein Themis promote the activation and proliferation of peripheral γδIFN cells in response to T cell antigen receptor stimulation in vitro and to Plasmodium infection in vivo. This resource can help to understand the distinct activities of effector γδ T cell subsets in pathophysiology.eng
dc.description.sponsorshipThe project leading to these results has received funding from the European Research Council under the European Union’s Horizon 2020 research and innovation programme (grant agreement number 646701 to B.S.-S.), ‘la Caixa’ Foundation under project code LCF/PR/HR24/00929 (to B.S.-S.), Fundação para a Ciência e Tecnologia, Ministério da Ciência, Tecnologia e Ensino Superior, Portugal (PTDC/MED-IMU/32296/2017 to A.M.C.; SFRH/BD/145352/2019 to D.I.; Decree-law number 57/2016, as amended by the Law number 57/2017 to A.P.) and Fondation pour la Recherche Médicale (to R.L.).
dc.identifier.citationInácio D, Amado T, Pamplona A, Sobral D, Cunha C, Gomes AQ, et al. Signature cytokine-associated transcriptome analysis of effector γδ T cells identifies subset-specific regulators of peripheral activation. Nat Immunol. 2025;26(3):497-510.
dc.identifier.doi10.1038/s41590-024-02073-8
dc.identifier.issn1529-2908
dc.identifier.issn1529-2916
dc.identifier.urihttp://hdl.handle.net/10400.21/21524
dc.language.isoeng
dc.peerreviewedyes
dc.publisherSpringer Nature
dc.relation.hasversionhttps://www.nature.com/articles/s41590-024-02073-8
dc.relation.ispartofNature Immunology
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectCytokines
dc.subjectGamma delta T cells
dc.titleSignature cytokine-associated transcriptome analysis of effector γδ T cells identifies subset-specific regulators of peripheral activationeng
dc.typejournal article
degois.publication.firstPage497
degois.publication.issue3
degois.publication.lastPage510
degois.publication.volume26
dspace.entity.typePublication
oaire.citation.titleNature Immunology
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameGomes
person.givenNameAnita
person.identifier.ciencia-id4B10-E015-52B7
person.identifier.orcid0000-0002-3348-0448
person.identifier.ridC-3580-2014
person.identifier.scopus-author-id7202386033
relation.isAuthorOfPublication2b5a3f0a-29c2-4ecd-a83f-50d0b99a4875
relation.isAuthorOfPublication.latestForDiscovery2b5a3f0a-29c2-4ecd-a83f-50d0b99a4875

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