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Novel polyol-responsive monoclonal antibodies against extracellular b-D-Glucans from pleurotus ostreatus

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dc.contributor.authorSemedo, Magda
dc.contributor.authorKarmali, Amin
dc.contributor.authorMartins, Sónia
dc.contributor.authorFonseca, Luís P.
dc.date.accessioned2019-03-12T12:30:51Z
dc.date.available2019-03-12T12:30:51Z
dc.date.issued2016
dc.description.abstractb-D-glucans from mushroom strains play a major role as biological response modifiers in several clinical disorders. Therefore, a specific assay method is of critical importance to find useful and novel sources of b-D-glucans with anti-tumor activity. Hybridoma technology was used to raise monoclonal antibodies (Mabs) against extracellular b-D-glucans (EBG) from Pleurotus ostreatus. Two of these hybridoma clones (3F8_3H7 and 1E6_1E8_B3) secreting Mabs against EBG from P. ostreatus were selected and 3F8_3H7 was used to investigate if they are polyol-responsive Mabs (PR-Mabs) by using ELlSA-elution assay. This hybridoma cell line secreted Mab of IgM class, which was purified in a single step by gel filtration chro-matography on Sephacryl S-300HR, which revealed a protein band on native PAGE with Mr of 917 kDa. Specificity studies of Mab 3F8_3H7 revealed that it recognized a common epitope on several b-D-glucans from different basidiomycete strains as determined by indirect EllSA and Western blotting under native conditions. This Mab exhibited high apparent affinity con-stant (KApp) for b-D-glucans from several mushroom strains. However, it revealed differential reactivity to some heat-treated b-D-glucans compared with the native forms suggesting that it binds to a conformation-sensitive epitope on b-D-glucan molecule. Epitope analysis of Mab 3F8_3H7 and 1E6_1E8_B3 was investigated by additivity index parameter, which revealed that they bound to the same epitope on some b-D-glucans and to different epitopes in other antigens. Therefore, these Mab can be used to assay for b-D-glucans as well as to act as powerful probes to detect conformational changes in these biopolymers.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSEMEDO, Magda C.; [et al] – Novel polyol-responsive monoclonal antibodies against extracellular b-D-Glucans from pleurotus ostreatus. Biotechnology Progress. ISSN 1520-6033. Vol. 32, N.º 1 (2016), pp. 116-125pt_PT
dc.identifier.doi10.1002/btpr.2196pt_PT
dc.identifier.issn1520-6033
dc.identifier.urihttp://hdl.handle.net/10400.21/9711
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWileypt_PT
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/doi/epdf/10.1002/btpr.2196pt_PT
dc.subjectHybridoma technologypt_PT
dc.subjectIgM Mab classpt_PT
dc.subjectExtracellular b-D-glucanspt_PT
dc.subjectPleurotus ostreatuspt_PT
dc.subjectEpitope analysis of Mabpt_PT
dc.titleNovel polyol-responsive monoclonal antibodies against extracellular b-D-Glucans from pleurotus ostreatuspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage125pt_PT
oaire.citation.issue1pt_PT
oaire.citation.startPage116pt_PT
oaire.citation.titleBiotechnology Progresspt_PT
oaire.citation.volume32pt_PT
person.familyNameSemedo
person.familyNameKarmali
person.familyNameMartins
person.givenNameMagda
person.givenNameAmin
person.givenNameSónia
person.identifier1022083
person.identifier1015604
person.identifier.ciencia-id9C11-BD02-C77F
person.identifier.ciencia-id2F17-581A-D4F8
person.identifier.orcid0000-0001-6330-2899
person.identifier.orcid0000-0003-0419-401X
person.identifier.orcid0000-0002-1874-0192
person.identifier.ridE-4787-2011
person.identifier.ridA-5945-2011
person.identifier.scopus-author-id57000381900
person.identifier.scopus-author-id6603778216
person.identifier.scopus-author-id8728101400
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication73e398bc-a0e8-420d-81eb-34159398838d
relation.isAuthorOfPublication23975b4b-3234-4139-9675-89ea6f9a2332
relation.isAuthorOfPublicationf8015c40-c2aa-45f3-b40b-e2ddab99d2a0
relation.isAuthorOfPublication.latestForDiscovery23975b4b-3234-4139-9675-89ea6f9a2332

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