| Nome: | Descrição: | Tamanho: | Formato: | |
|---|---|---|---|---|
| 6.5 MB | Adobe PDF |
Orientador(es)
Resumo(s)
Infections associated with the surfaces of medical devices represent a critical problem due to biofilm formation and the growing resistance towards antibacterial drugs. This is particularly relevant in commonly used invasive devices such as silicone-based ones where a demand for alternative antibiofilm surfaces is increasing. In this work, an antimicrobial chitosan-biosurfactant hydrogel mesh was produced by 3D-printing. The 3D structure was designed to coat polydimethylsiloxane-based medical devices for infection prevention. Additionally, the porous 3D structure allows the incorporation of customized bioactive components. For this purpose, two biosurfactants (surfactin and sophorolipids) were biosynthesized and tested for their antimicrobial activity. In addition, the printing of surfactant-chitosan-based coatings was optimized, and the resulting 3D structures were characterized (i.e., wettability, FTIR-ATR, antimicrobial activity, and biocompatibility). Compared with surfactin, the results showed a better yield and higher antibacterial activity against Gram-positive bacteria for sophorolipids (SLs). Thus, SLs were used to produce chitosan-based 3D-printed coatings. Overall, the SLs-impregnated coatings showed the best antibacterial activity against Staphylococcus aureus planktonic bacteria (61 % of growth inhibition) and antibiofilm activity (2 log units reduction) when compared to control. Furthermore, concerning biocompatibility, the coatings were cytocompatible towards human dermal fibroblasts. Finally, the coating presented a mesh suitable to be filled with a model bioactive compound (i.e., hyaluronic acid), paving the way to be used for customized therapeutics.
Descrição
Palavras-chave
Polymer-coating PDMS Mesh Additive manufacturing Sophorolipids Surfactin
Contexto Educativo
Citação
Francisco Narciso et al. (2023), 3D-printed biosurfactant-chitosan antibacterial coating for the prevention of silicone-based associated infections, Colloids and surfaces B: biointerfaces, Volume 230, 2023, 113486, ISSN 0927-7765, https://doi.org/10.1016/j.colsurfb.2023.113486
Editora
Elsevier