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Bioburden assessment by passive methods on a clinical pathology service in one central hospital from Lisbon: what can it tell us regarding patients and staff exposure?

2020-04Journal article Open access
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dc.contributor.authorViegas, Carla
dc.contributor.authorTwarużek, Magdalena
dc.contributor.authorLourenço, Raquel
dc.contributor.authorDias, Marta
dc.contributor.authorAlmeida, Beatriz
dc.contributor.authorAranha Caetano, Liliana
dc.contributor.authorCarolino, Elisabete
dc.contributor.authorGomes, Anita Q.
dc.contributor.authorKosicki, Robert
dc.contributor.authorSoszczyńska, Ewelina
dc.contributor.authorViegas, Susana
dc.date.accessioned2020-04-06T11:28:28Z
dc.date.available2020-04-06T11:28:28Z
dc.date.issued2020-04
dc.descriptionProject EXPOsE. Project nº 23222 (02/SAICT/2016).pt_PT
dc.description.abstractThe assessment and control of microbial contamination in healthcare facilities is presently a mandatory and vital part of strategies to prevent and control hospital-acquired infections. This study aims to assess the bioburden with two passive sampling methods (30 ventilations grids swabs and 16 electrostatic dust collectors (EDCs)) at Clinical Pathology Services. The fungal burden was characterized through molecular tools, antifungal resistance, and the mycotoxins and cytotoxicity profile. Total bacteria presented the highest prevalence in both matrixes, whereas Gram-bacteria presented the lowest. Swabs presented a higher prevalence (27.6%) for fungal burden. Chrysonilia sitophila presented the highest prevalence in swabs, whereas for EDCs, C. sitophila and Mucor sp. were the most prevalent. Concerning Aspergillus genera on swabs, section Flavi was the one with the highest prevalence (58.02%), whereas, for EDCs, section Versicolores was the only section observed (100%). Aspergillus section Fumigati was detected in 10 swabs and 7 EDC samples and Aspergillus section Versicolores were detected in one EDC sample. Fungal growth on azole-supplemented media was observed in eight EDC samples. No mycotoxins were detected in any of the samples. A low cytotoxic effect was observed in two sites upon incubation of collected samples with A549 and SK cells and in two other sites upon incubation of collected samples with SK cells only. A medium cytotoxic effect was observed with one EDC sample upon incubation with A549 cells. This study reinforces the need for determination of the azole resistance profile for fungal species and allowed a preliminary risk characterization regarding the cytotoxicity. An intervention including the use of ultraviolet with a wavelength between 200 nm and 280 nm (UVC)—emitting device and increased maintenance and cleaning of the central heating, ventilation, and air conditioning (HVAC) systems should be ensured to promote the reduction of microbial contamination.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationViegas C, Twarużek M, Caetano LA, Carolino E, Gomes AQ, Viegas S, et al. Bioburden assessment by passive methods on a clinical pathology service in one central hospital from Lisbon: what can it tell us regarding patients and staff exposure? Atmosphere. 2020;11(4):351.pt_PT
dc.identifier.doi10.3390/atmos11040351pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/11415
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationProject EXPOsEpt_PT
dc.relationProject nº 23222 (02/SAICT/2016)pt_PT
dc.relation.publisherversionhttps://www.mdpi.com/2073-4433/11/4/351pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectOccupational healthpt_PT
dc.subjectOccupational exposurept_PT
dc.subjectBioburdenpt_PT
dc.subjectPassive methodspt_PT
dc.subjectAspergilluspt_PT
dc.subjectAzole-resistance profilept_PT
dc.subjectCytotoxicity analysispt_PT
dc.subjectProject EXPOsEpt_PT
dc.subjectProject nº 23222 (02/SAICT/2016)pt_PT
dc.subjectPortugalpt_PT
dc.subjectLisboapt_PT
dc.titleBioburden assessment by passive methods on a clinical pathology service in one central hospital from Lisbon: what can it tell us regarding patients and staff exposure?pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue4pt_PT
oaire.citation.startPage351pt_PT
oaire.citation.titleAtmospherept_PT
oaire.citation.volume11pt_PT
person.familyNameViegas
person.familyNameAranha Caetano
person.familyNameCarolino
person.familyNameGomes
person.familyNameViegas
person.givenNameCarla
person.givenNameLiliana
person.givenNameElisabete
person.givenNameAnita
person.givenNameSusana
person.identifier248817
person.identifier.ciencia-idEE1E-C639-D70F
person.identifier.ciencia-id9716-9DAC-532A
person.identifier.ciencia-id1216-EFA3-1E0F
person.identifier.ciencia-id4B10-E015-52B7
person.identifier.ciencia-idA919-7318-63DC
person.identifier.orcid0000-0002-1545-6479
person.identifier.orcid0000-0003-1496-2609
person.identifier.orcid0000-0003-4165-7052
person.identifier.orcid0000-0002-3348-0448
person.identifier.orcid0000-0003-1015-8760
person.identifier.ridB-7217-2013
person.identifier.ridF-1012-2015
person.identifier.ridC-3580-2014
person.identifier.ridI-4053-2012
person.identifier.scopus-author-id55443609700
person.identifier.scopus-author-id25821697000
person.identifier.scopus-author-id7202386033
person.identifier.scopus-author-id35270591500
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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