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Occupational exposure to bioburden in Portuguese bakeries: an approach to sampling viable microbial load

2018-06Journal article Open access
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dc.contributor.authorViegas, Carla
dc.contributor.authorMonteiro, Ana
dc.contributor.authorCarolino, Elisabete
dc.contributor.authorViegas, Susana
dc.date.accessioned2018-10-18T15:53:43Z
dc.date.available2018-10-18T15:53:43Z
dc.date.issued2018-06
dc.description.abstractIn bakeries, a number of operations such as mixing are associated with exposure to air-suspended flour dust and related bioburden. The aim of this study was to find the best active sampling approach to the assessment of occupational exposure to bioburden in Portuguese bakeries based on the data obtained with the use of specific impaction and impinger devices. We used impaction to collect fungal particles from 100 L air samples onto malt extract agar (MEA) supplemented with chloramphenicol (0.05 %). For growing fungi we also used dichloran glycerol (DG18) agar-based media and for mesophilic bacteria we used tryptic soy agar (TSA) supplemented with nystatin (0.2 %). For Enterobacteriaceae we used violet red bile agar (VRBA). With impingers we also collected 300 L air samples at the 300 L/min airflow rate, inoculated onto the same culture media. The two methods, impaction and impinger, showed statistically significant differences in the following counts: fungal on MEA (z=-2.721, p=0.007), fungal on DG18 (z=-4.830, p=0.000), total bacteria (z=-5.435, p=0.000), and Gram-negative coliforms (z=-3.716, p=0.000). In all cases the impaction method detected significantly higher concentrations than the impinger method. Fungal and bacterial loads were higher in the production unit and lower in the shop. The fungal load obtained with impaction varied between 10 and 5140 CFU m-3, and total bacterial counts ranged between 10 and 4120 CFU m-3. This study has shown that the impaction method is the best active sampling approach to assessing viable bioburden in this specific occupational environment, but a multi-faceted approach to sampling and analyses combining methods and media enables a more refined risk characterisation and, consequently, better tailored risk control measures to reduce adverse health outcomes in workers.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationViegas C, Monteiro A, Carolino E, Viegas S. Occupational exposure to bioburden in Portuguese bakeries: an approach to sampling viable microbial load. Arh Hig Rada Toksikol. 2018;69(3):250-7.pt_PT
dc.identifier.doi10.2478/aiht-2018-69-3116pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/8946
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherDe Gruyterpt_PT
dc.relation.publisherversionhttps://www.degruyter.com/view/j/aiht.2018.69.issue-3/aiht-2018-69-3116/aiht-2018-69-3116.xmlpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectOccupational healthpt_PT
dc.subjectOccupational exposurept_PT
dc.subjectBacteriapt_PT
dc.subjectBioburdenpt_PT
dc.subjectFungipt_PT
dc.subjectImpaction, impingerpt_PT
dc.subjectMalt extract agarpt_PT
dc.subjectDG18 agarpt_PT
dc.subjectTryptic soy agarpt_PT
dc.subjectViolet red bile agarpt_PT
dc.subjectBakerypt_PT
dc.subjectPortugalpt_PT
dc.titleOccupational exposure to bioburden in Portuguese bakeries: an approach to sampling viable microbial loadpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage257pt_PT
oaire.citation.issue3pt_PT
oaire.citation.startPage250pt_PT
oaire.citation.titleArchives of Industrial Hygiene and Toxicologypt_PT
oaire.citation.volume69pt_PT
person.familyNameViegas
person.familyNameCarolino
person.familyNameViegas
person.givenNameCarla
person.givenNameElisabete
person.givenNameSusana
person.identifier248817
person.identifier.ciencia-idEE1E-C639-D70F
person.identifier.ciencia-id1216-EFA3-1E0F
person.identifier.ciencia-idA919-7318-63DC
person.identifier.orcid0000-0002-1545-6479
person.identifier.orcid0000-0003-4165-7052
person.identifier.orcid0000-0003-1015-8760
person.identifier.ridB-7217-2013
person.identifier.ridF-1012-2015
person.identifier.ridI-4053-2012
person.identifier.scopus-author-id55443609700
person.identifier.scopus-author-id25821697000
person.identifier.scopus-author-id35270591500
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationb5fa5da4-50c3-4b88-ae20-1bc63cb485f7
relation.isAuthorOfPublication77930d39-ed34-44dc-a4a6-9bf833e5e688
relation.isAuthorOfPublication13115332-43f7-4048-a8a5-2f2b855a8c92
relation.isAuthorOfPublication.latestForDiscoveryb5fa5da4-50c3-4b88-ae20-1bc63cb485f7

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