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Dual-loaded chitosan-based nanoparticles: a novel approach for treating polymicrobial osteomyelitis

2025-04Journal article Open access
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datacite.subject.sdg03:Saúde de Qualidade
dc.contributor.authorZegre, Miguel
dc.contributor.authorBarros, J.
dc.contributor.authorDavid, A. B.
dc.contributor.authorFialho, L.
dc.contributor.authorFerraz, M. P.
dc.contributor.authorMonteiro, F. J.
dc.contributor.authorCaetano, Liliana Aranha
dc.contributor.authorGonçalves, L.
dc.contributor.authorBettencourt, A.
dc.date.accessioned2025-04-02T11:10:16Z
dc.date.available2025-04-02T11:10:16Z
dc.date.issued2025-04
dc.descriptionThe authors thank the Fundação para a Ciência e Tecnologia (FCT), Portugal for the financial support of the projects: UIDB/04138/2020 and UIDP/04138/2020 (iMed.Ulisboa); UIDB/05608/2020 and UIDP/05608/2020 (H&TRC). L. Gonçalves, Principal Researcher grant (CEECIND/03143/2017); J. Barros, Junior Researcher Grant (2022.05157.CEECIND/CP1735 /CT0011) and thanks to project Agenda “Health from Portugal - HfPT” [C630926586-00465198], co-financed by the Recovery and Resilience Plan (RRP) and by the European funds NextGeneration EU (https://recuperarportugal.gov.pt/), through the Incentive System “Agendas for Business Innovation”. The Graphical Abstract was created with Biorender.com.
dc.description.abstractDeveloping innovative approaches to target osteomyelitis caused by polymicrobial infections remains a significant therapeutic challenge. In this study, monodispersed chitosan nanoparticles co-loaded with antibacterial (minocycline) and antifungal (voriconazole) agents were successfully prepared. Minocycline presented higher encapsulation efficiency as compared to voriconazole. Thermostability analysis suggested interactions between the co-loaded drugs within the dual-delivery system, potentially limiting voriconazole release. The dual-loaded chitosan nanoparticles exhibited significant in vitro anti-biofilm activity, achieving up to a 90% reduction in polymicrobial biofilms of S. aureus and C. albicans. Additionally, the nanoparticles showed cytocompatibility with a human osteoblast cell line. These findings highlight the potential of this dual-delivery chitosan-based nanoparticle system to address a critical gap in osteomyelitis treatment by targeting both bacterial and fungal pathogens.eng
dc.identifier.citationZegre M, Barros J, David AB, Fialho L, Ferraz MP, Caetano LA, et al. Dual-loaded chitosan-based nanoparticles: a novel approach for treating polymicrobial osteomyelitis. Int J Pharm. 2025;674:125480.
dc.identifier.doi10.1016/j.ijpharm.2025.125480
dc.identifier.issn0378-5173
dc.identifier.urihttp://hdl.handle.net/10400.21/21735
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.relation.hasversionhttps://www.sciencedirect.com/science/article/pii/S0378517325003163
dc.relation.ispartofInternational Journal of Pharmaceutics
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectBiofilm infections
dc.subjectChitosan
dc.subjectDrug delivery system
dc.subjectMinocycline
dc.subjectMinocycline hydrochloride
dc.subjectMixed osteomyelitis
dc.subjectPolysaccharide nanoparticles
dc.subjectPoloxamer
dc.subjectVoriconazole
dc.subjectFCT_UIDB/05608/2020
dc.subjectFCT_UIDP/05608/2020
dc.titleDual-loaded chitosan-based nanoparticles: a novel approach for treating polymicrobial osteomyelitiseng
dc.typejournal article
dspace.entity.typePublication
oaire.citation.startPage125480
oaire.citation.titleInternational Journal of Pharmaceutics
oaire.citation.volume674
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameAranha Caetano
person.givenNameLiliana
person.identifier.ciencia-id9716-9DAC-532A
person.identifier.orcid0000-0003-1496-2609
relation.isAuthorOfPublication6517c656-f913-4f54-8682-77c2856c9e4c
relation.isAuthorOfPublication.latestForDiscovery6517c656-f913-4f54-8682-77c2856c9e4c

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