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Plasma versus serum analysis by FTIR spectroscopy to capture the human physiological state

2022-12Artigo científico Acesso aberto
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dc.contributor.authorAraújo, Rúben
dc.contributor.authorRamalhete, Luís
dc.contributor.authorRibeiro, Edna
dc.contributor.authorCalado, Cecília
dc.date.accessioned2022-12-28T13:12:16Z
dc.date.available2022-12-28T13:12:16Z
dc.date.issued2022-12
dc.description.abstractFourier Transform InfraRed spectroscopy of serum and plasma has been highly explored for medical diagnosis, due to its general simplicity, and high sensitivity and specificity. To evaluate the plasma and serum molecular fingerprint, as obtained by FTIR spectroscopy, to acquire the system metabolic state, serum and plasma spectra were compared to characterize the metabolic state of 30 human volunteers, between 90 days of consumption of green tea extract rich in Epigallocatechin-3-gallate (EGCG). Both plasma and serum spectra enabled the high impact of EGCG consumption on the biofluid spectra to be observed, as analyzed by the spectra principal component analysis, hierarchical-cluster analysis, and univariate data analysis. Plasma spectra resulted in the prediction of EGCG consumption with a slightly higher specificity, accuracy, and precision, also pointing to a higher number of significant spectral bands that were different between the 90 days period. Despite this, the lipid regions of the serum spectra were more affected by EGCG consumption than the corresponding plasma spectra. Therefore, in general, if no specific compound analysis is highlighted, plasma is in general the advised biofluid to capture by FTIR spectroscopy the general metabolic state. If the lipid content of the biofluid is relevant, serum spectra could present some advantages over plasma spectra.pt_PT
dc.description.sponsorshipThe present work was conducted at H&TRCHealth & Technology Research Center, Escola Superior de Tecnologia da Saúde de Lisboa, Instituto Politécnico de Lisboa, and in the Engineering and Health Laboratory, that resulted from a collaboration protocol established between Universidade Católica Portuguesa and Instituto Politécnico de Lisboa.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAraújo R, Ramalhete L, Ribeiro E, Calado C. Plasma versus serum analysis by FTIR spectroscopy to capture the human physiological state. BioTech (Basel). 2022;11(4):56.pt_PT
dc.identifier.doi10.3390/biotech11040056pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/15210
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relation.publisherversionhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9775178/pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectFTIR spectroscopypt_PT
dc.subjectEpigallocatechin-3-gallatept_PT
dc.subjectPlasmapt_PT
dc.subjectSerumpt_PT
dc.titlePlasma versus serum analysis by FTIR spectroscopy to capture the human physiological statept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue4pt_PT
oaire.citation.startPage56pt_PT
oaire.citation.titleBioTechpt_PT
oaire.citation.volume11pt_PT
person.familyNameRibeiro
person.givenNameEdna
person.identifier.ciencia-idC414-CDF2-D35A
person.identifier.orcid0000-0003-1316-7750
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationa571bf34-bcda-49ca-b5cb-4cdecbb3d9c7
relation.isAuthorOfPublication.latestForDiscoverya571bf34-bcda-49ca-b5cb-4cdecbb3d9c7

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