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Six feet under microbiota: microbiologic contamination and toxicity profile in three urban cemeteries from Lisbon, Portugal

2022-05Journal article Open access
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dc.contributor.authorViegas, Carla
dc.contributor.authorCervantes, Renata
dc.contributor.authorDias, Marta
dc.contributor.authorGomes, Bianca
dc.contributor.authorPena, Pedro
dc.contributor.authorCarolino, Elisabete
dc.contributor.authorTwarużek, Magdalena
dc.contributor.authorKosicki, Robert
dc.contributor.authorSoszczyńska, Ewelina
dc.contributor.authorViegas, Susana
dc.contributor.authorCaetano, Liliana Aranha
dc.date.accessioned2022-05-23T10:54:22Z
dc.date.available2022-05-23T10:54:22Z
dc.date.issued2022-05
dc.descriptionEEA Grants Project “EXPOGreen FBR38”.pt_PT
dc.descriptionProject “Occupational exposure of ambulance drivers to bioburden” (IPL/2020/BIOAmbuDrivers_ESTeSL).pt_PT
dc.descriptionFCT_UIDB/05608/2020. FCT_UIDP/05608/2020.pt_PT
dc.description.abstractCemeteries are potential environmental reservoirs of pathogenic microorganisms from organic matter decomposition. This study aimed to characterize the microbial contamination in three cemeteries, and more specifically in grave diggers’ facilities. One active sampling method (impingement method) and several passive sampling methods (swabs, settled dust, settled dust filters, and electrostatic dust cloths—EDC) were employed. The molecular detection of Aspergillus sections and SARS-CoV-2, as well as mycotoxin analysis, screening of azole resistance, and cytotoxicity measurement were also conducted. Total bacteria contamination was 80 CFU·m−2 in settled dust samples, reached 849 CFU·m−2 in EDC and 20,000 CFU·m−2 in swabs, and ranged from 5000 to 10,000 CFU·m−2 in filters. Gram-negative bacteria (VRBA) were only observed in settled dust samples (2.00 × 105 CFU·m−2). Regarding Aspergillus sp., the highest counts were obtained in DG18 (18.38%) and it was not observed in azole-supplemented SDA media. SARS-CoV-2 and the targeted Aspergillus sections were not detected. Mycophenolic acid was detected in one settled dust sample. Cytotoxic effects were observed for 94.4% filters and 5.6% EDC in A549 lung epithelial cells, and for 50.0% filters and 5.6% EDC in HepG2 cells. Future studies are needed in this occupational setting to implement more focused risk management measures.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationViegas C, Cervantes R, Dias M, Gomes B, Pena P, Carolino E, Viegas C, Caetano LA, et al. Six feet under microbiota: microbiologic contamination and toxicity profile in three urban cemeteries from Lisbon, Portugal. Toxins. 2022;14(5):348.pt_PT
dc.identifier.doi10.3390/toxins14050348pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/14651
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationEXPOGreen FBR38pt_PT
dc.relationProject “Occupational exposure of ambulance drivers to bioburden” (IPL/2020/BIOAmbuDrivers_ESTeSL)pt_PT
dc.relation.publisherversionhttps://www.mdpi.com/2072-6651/14/5/348pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectOccupational healthpt_PT
dc.subjectOccupational exposurept_PT
dc.subjectCemeteriespt_PT
dc.subjectAspergilluspt_PT
dc.subjectSARS-CoV-2pt_PT
dc.subjectAzole resistancept_PT
dc.subjectMycotoxinspt_PT
dc.subjectCytotoxicitypt_PT
dc.subjectEXPOGreen FBR38pt_PT
dc.subjectIPL/2020/BIOAmbuDrivers_ESTeSLpt_PT
dc.subjectFCT_UIDB/05608/2020pt_PT
dc.subjectFCT_UIDP/05608/2020pt_PT
dc.titleSix feet under microbiota: microbiologic contamination and toxicity profile in three urban cemeteries from Lisbon, Portugalpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue5pt_PT
oaire.citation.startPage348pt_PT
oaire.citation.titleToxinspt_PT
oaire.citation.volume14pt_PT
person.familyNameViegas
person.familyNameWellenkamp de Carvalho Freire de Cervantes
person.familyNameDias
person.familyNameGomes
person.familyNamePena
person.familyNameCarolino
person.familyNameViegas
person.familyNameAranha Caetano
person.givenNameCarla
person.givenNameRenata
person.givenNameMarta
person.givenNameBianca
person.givenNamePedro
person.givenNameElisabete
person.givenNameSusana
person.givenNameLiliana
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rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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