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Advisor(s)
Abstract(s)
The crucial role of murine γδ T cells in several (patho)physiological contexts stems from a complex process of ‘developmental preprogramming’ in the thymus, after which a significant fraction of γδ T cells populate peripheral sites already endowed with the capacity to secrete either IL-17 or IFN-γ1. However, despite the relevance of these γδ T cell effector subsets, we still lack knowledge on the transcriptomes that specifically associate with IL-17 or IFN-γ production. To address this, we established a double reporter IL-17-GFP:IFN-γ-YFP mouse strain, which allowed us to isolate pure peripheral IL-17-producing (γδ17) or IFN-γ-producing (γδIFN) γδ T cells to perform RNA-sequencing.
Description
Keywords
IFN-γ IL-17-specific transcriptomes γδ T lymphocytes Themis
Citation
Inácio D, Amado T, Pamplona A, Sobral D, Cunha C, Gomes AQ, et al. Dissecting the IFN-g versus IL-17-specific transcriptomes of effector gd T lymphocytes: a new role for signalling adaptor Themis. In: XLVIII Annual Meeting of the Portuguese Society for Immunology, Aveiro Congress Center (Aveiro), March 29-31, 2023.