Logo do repositório
 
Publicação

MicroRNA-181a regulates IFN-γ expression in effector CD8+ T cell differentiation

2020-01Artigo científico Acesso aberto
Ver registo simples
dc.contributor.authorAmado, Tiago
dc.contributor.authorAmorim, Ana
dc.contributor.authorEnguita, Francisco J.
dc.contributor.authorRomero, Paula V.
dc.contributor.authorInácio, Daniel
dc.contributor.authorMiranda, Marta Pires
dc.contributor.authorWinter, Samantha J.
dc.contributor.authorSimas, J. Pedro
dc.contributor.authorKrueger, Andreas
dc.contributor.authorSchmolka, Nina
dc.contributor.authorSilva-Santos, Bruno
dc.contributor.authorGomes, Anita Q.
dc.date.accessioned2020-02-19T12:10:46Z
dc.date.available2020-02-19T12:10:46Z
dc.date.issued2020-01
dc.description.abstractCD8+ T cells are key players in immunity against intracellular infections and tumors. The main cytokine associated with these protective responses is interferon-γ (IFN-γ), whose production is known to be regulated at the transcriptional level during CD8+ T cell differentiation. Here we found that microRNAs constitute a posttranscriptional brake to IFN-γ expression by CD8+ T cells since the genetic interference with the Dicer processing machinery resulted in the overproduction of IFN-γ by both thymic and peripheral CD8+ T cells. Using a gene reporter mouse for IFN-γ locus activity, we compared the microRNA repertoires associated with the presence or absence of IFN-γ expression. This allowed us to identify a set of candidates, including miR-181a and miR-451, which were functionally tested in overexpression experiments using synthetic mimics in peripheral CD8+ T cell cultures. We found that miR-181a limits IFN-γ production by suppressing the expression of the transcription factor Id2, which in turn promotes the Ifng expression program. Importantly, upon the MuHV-4 challenge, miR-181a-deficient mice showed a more vigorous IFN-γ+ CD8+ T cell response and were able to control viral infection significantly more efficiently than control mice. These data collectively establish a novel role for miR-181a in regulating IFN-γ-mediated effector CD8+ T cell responses in vitro and in vivo.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAmado T, Amorim A, Enguita FJ, Romero PV, Inácio D, Gomes AQ, et al. MicroRNA-181a regulates IFN-γ expression in effector CD8+ T cell differentiation. J Mol Med. 2020;98(2):309-20.pt_PT
dc.identifier.doi10.1007/s00109-019-01865-ypt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/11115
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.relation.publisherversionhttps://link.springer.com/article/10.1007%2Fs00109-019-01865-ypt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectMicroRNA-181apt_PT
dc.subjectIFN-γ expressionpt_PT
dc.subjectCD8+ T cellpt_PT
dc.titleMicroRNA-181a regulates IFN-γ expression in effector CD8+ T cell differentiationpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage320pt_PT
oaire.citation.issue2pt_PT
oaire.citation.startPage309pt_PT
oaire.citation.titleJournal of Molecular Medicinept_PT
oaire.citation.volume98pt_PT
person.familyNameGomes
person.givenNameAnita
person.identifier.ciencia-id4B10-E015-52B7
person.identifier.orcid0000-0002-3348-0448
person.identifier.ridC-3580-2014
person.identifier.scopus-author-id7202386033
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication2b5a3f0a-29c2-4ecd-a83f-50d0b99a4875
relation.isAuthorOfPublication.latestForDiscovery2b5a3f0a-29c2-4ecd-a83f-50d0b99a4875

Ficheiros

Principais
A mostrar 1 - 1 de 1
Miniatura indisponível
Nome:
MicroRNA-181a regulates IFN-γ expression in effector CD8+ T cell differentiation.pdf
Tamanho:
867.49 KB
Formato:
Adobe Portable Document Format
Ver/Abrir
Licença
A mostrar 1 - 1 de 1
Miniatura indisponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Ver/Abrir

Coleções

ESTeSL - Artigos