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Genotoxicity biomarkers in occupational exposure to formaldehyde: the case of histopathology laboratories

2011-01Journal article Open access
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dc.contributor.authorLadeira, Carina
dc.contributor.authorViegas, Susana
dc.contributor.authorCarolino, Elisabete
dc.contributor.authorPrista, João
dc.contributor.authorGomes, Manuel C.
dc.contributor.authorBrito, Miguel
dc.date.accessioned2011-11-03T14:00:36Z
dc.date.available2011-11-03T14:00:36Z
dc.date.issued2011-01
dc.description.abstractFormaldehyde, classified by the IARC as carcinogenic in humans and experimental animals, is a chemical agent that is widely used in histopathology laboratories. The exposure to this substance is epidemiologically linked to cancer and to nuclear changes detected by the cytokinesis-block micronucleus test (CBMN). This method is extensively used in molecular epidemiology, since it provides information on several biomarkers of genotoxicity, such as micronuclei (MN), which are biomarkers of chromosomes breakage or loss, nucleoplasmic bridges (NPB), common biomarkers of chromosome rearrangement, poor repair and/or telomere fusion, and nuclear buds (NBUD), biomarkers of elimination of amplified DNA. The aim of this study is to compare the frequency of genotoxicity biomarkers, provided by the CBMN assay in peripheral lymphocytes and the MN test in buccal cells, between individuals occupationally exposed and non-exposed to formaldehyde and other environmental factors, namely tobacco and alcohol consumption. The sample comprised two groups: 56 individuals occupationally exposed to formaldehyde (cases) and 85 unexposed individuals (controls), from whom both peripheral blood and exfoliated epithelial cells of the oral mucosa were collected in order to measure the genetic endpoints proposed in this study. The mean level of TWA8h was 0.16±0.11ppm (<detection limit until 0.51 ppm) and the mean of ceiling values was 1.14±0.74ppm (0.18–2.93 ppm). All genotoxicity biomarkers showed significant increases in exposed workers in comparison with controls (Mann–Whitney test, p < 0.002) and the analysis of confounding factors showed that there were no differences between genders. As for age, only the mean MN frequency in lymphocytes was found significantly higher in elderly people among the exposed groups (p = 0.006), and there was also evidence of an interaction between age and gender with regards to that biomarker in those exposed. Smoking habits did not influence the frequency of the biomarkers, whereas alcohol consumption only influenced the MN frequency in lymphocytes in controls (p = 0.011), with drinkers showing higher mean values. These results provide evidence of the association between occupational exposure to formaldehyde and the presence of genotoxicity biomarkers.por
dc.identifier.citationLadeira C, Viegas S, Carolino E, Prista J, Gomes MC, Brito M. Genotoxicity biomarkers in occupational exposure to formaldehyde: the case of histopathology laboratories. Mutation Research - Genetic Toxicology and Environmental Mutagenesis. 2011;721(1):15-20.por
dc.identifier.issn1383-5718
dc.identifier.urihttp://hdl.handle.net/10400.21/214
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S1383571811000155por
dc.subjectSaúde ocupacionalpor
dc.subjectFormaldeídopor
dc.subjectGenotoxicidadepor
dc.subjectMeio laboratorialpor
dc.subjectAnatomia patológicapor
dc.titleGenotoxicity biomarkers in occupational exposure to formaldehyde: the case of histopathology laboratoriespor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage20por
oaire.citation.startPage15por
oaire.citation.titleMutation Research - Genetic Toxicology and Environmental Mutagenesispor
person.familyNameLadeira
person.familyNameViegas
person.familyNameCarolino
person.familyNameBrito
person.givenNameCarina
person.givenNameSusana
person.givenNameElisabete
person.givenNameMiguel
person.identifier144237
person.identifier248817
person.identifier.ciencia-id801C-1BBA-1D9E
person.identifier.ciencia-idA919-7318-63DC
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person.identifier.orcid0000-0001-5588-0074
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person.identifier.orcid0000-0003-4165-7052
person.identifier.orcid0000-0001-6394-658X
person.identifier.ridJ-2572-2012
person.identifier.ridI-4053-2012
person.identifier.ridF-1012-2015
person.identifier.ridA-7970-2016
person.identifier.scopus-author-id36463788000
person.identifier.scopus-author-id35270591500
person.identifier.scopus-author-id25821697000
person.identifier.scopus-author-id35224551000
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
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relation.isAuthorOfPublication.latestForDiscovery13115332-43f7-4048-a8a5-2f2b855a8c92

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