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Ovine model as a temporomandibular disc substitute: characterisation and the outcomes of freezing storage

2022-05-20Journal article Open access
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dc.contributor.authorTrindade, Daniela
dc.contributor.authorCalado, Cecília
dc.contributor.authorAlves, Nuno
dc.contributor.authorMoura, Carla
dc.date.accessioned2022-05-31T08:13:38Z
dc.date.available2022-05-31T08:13:38Z
dc.date.issued2022-05-20
dc.description.abstractThe temporomandibular joint (TMJ) is a ginglymoarthrodial joint that comprises a fibrocartilaginous disc that is highly predisposed to suffer from trauma or degenerative events, leading to disorders in the condyle–disc complex. The use of decellularized tissues has attracted interest in the tissue engineering (TE) field as an intact extracellular matrix can be obtained [1]. To this end, the ovine model was the first choice for a TMJ TE approach as they are easy to obtain, inexpensive and present an easily accessible surgical site [2]. When biological materials are used, their conservation should be taken into consideration, as sometimes it is not possible to test them immediately after extraction. With this, the present study aims to characterise the ovine disc, and determine its maximum freezing-time storage without alterations in its morphological and compression properties and biochemical composition. For this, two storage conditions were tested: (i) freezing at −20 ◦C in phosphate buffered saline (PBS) solution and thaw at 4 ◦C (PBS + 4 ◦C) and (ii) wrapping the discs in PBS embedded gauze and freezing at −20 ◦C followed by thaw at room temperature (RT) in PBS (Gauze + RT). Moreover, different time intervals were assessed: 1, 7 and 14 days. Results showed that the native disc presented a thickness of 1.62 ± 0.674 mm, weighted 0.385 ± 0.029 g and had a compressive modulus of 2.36 ± 0.072 MPa. Regarding biochemical composition, collagen content was higher in the central zone, whereas glycosaminoglycans were higher in the lateral and posterior zone. After performing both storage methods, morphological characteristics were minimally altered, but the biochemical content was significantly affected. After the 14-day experiments, there was an increase of about 30% in the compression modulus for Gauze + RT when compared to the fresh native disc, while for the PBS + 4 ◦C no changes were observed. This study determined that freezing the ovine discs may lead to changes in the native properties, indicating that when it is necessary to store them, these changes should be taken into consideration in future studies.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationTRINDADE, Daniela; [et al] – Ovine model as a temporomandibular disc substitute: characterisation and the outcomes of freezing storage. Materials Proceedings. eISSN 2673-4605. Vol. 8, N.º 1 (2022), pp. 1-2.pt_PT
dc.identifier.doi10.3390/materproc2022008017pt_PT
dc.identifier.eissn2673-4605
dc.identifier.urihttp://hdl.handle.net/10400.21/14676
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationLA/P/0112/2020 - Associate Laboratory ARISEpt_PT
dc.relationCentre for Rapid and Sustainable Product Development
dc.relationCentre for Rapid and Sustainable Product Development
dc.relationDesenvolvimento e implementação de um novo sistema para a produção de ortóteses tornozelo-pé customizadas para pacientes com acidente vascular cerebral ou paralisia cerebral
dc.relation.publisherversionhttps://www.mdpi.com/2673-4605/8/1/17pt_PT
dc.subjectTemporomandibular joint discpt_PT
dc.subjectOvine modelpt_PT
dc.subjectFreezing time storagept_PT
dc.subjectCompression propertiespt_PT
dc.subjectBiochemical compositionpt_PT
dc.titleOvine model as a temporomandibular disc substitute: characterisation and the outcomes of freezing storagept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre for Rapid and Sustainable Product Development
oaire.awardTitleCentre for Rapid and Sustainable Product Development
oaire.awardTitleDesenvolvimento e implementação de um novo sistema para a produção de ortóteses tornozelo-pé customizadas para pacientes com acidente vascular cerebral ou paralisia cerebral
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04044%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04044%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F145292%2F2019/PT
oaire.citation.endPage2pt_PT
oaire.citation.issue1pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleMaterials Proceedingspt_PT
oaire.citation.volume8pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamOE
person.familyNameTrindade
person.familyNameCalado
person.familyNameAlves
person.familyNameMonteiro de Moura
person.givenNameDaniela Alexandra
person.givenNameCecília
person.givenNameNuno
person.givenNameCarla Sofia
person.identifier130332
person.identifier452149
person.identifier.ciencia-id6316-1507-A39A
person.identifier.ciencia-id9418-E320-3177
person.identifier.ciencia-id311E-1559-8F6C
person.identifier.ciencia-id671C-8FEC-A14A
person.identifier.orcid0000-0003-3621-7095
person.identifier.orcid0000-0002-5264-9755
person.identifier.orcid0000-0002-5016-0868
person.identifier.orcid0000-0003-2610-1005
person.identifier.ridE-2102-2014
person.identifier.ridN-4073-2013
person.identifier.scopus-author-id6603163260
person.identifier.scopus-author-id7006403383
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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