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BCG-loaded chitosan microparticles: interaction with macrophages and preliminary in vivo studies

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dc.contributor.authorCaetano, Liliana Aranha
dc.contributor.authorFigueiredo, Lara
dc.contributor.authorAlmeida, António J.
dc.contributor.authorGonçalves, L. M. D.
dc.date.accessioned2017-06-09T14:48:43Z
dc.date.available2017-06-09T14:48:43Z
dc.date.issued2017
dc.description.abstractThe aim of this study was to develop a novel BCG-loaded chitosan vaccine with high association efficiency which can afford efficient interaction with APC and elicit local and Th1-type-specific immune response after intranasal administration. Chitosan-suspended BCG and BCG-loaded chitosan-alginate microparticles were prepared by ionotropic gelation. Interaction with APC was evaluated by fluorescence microscopy using rBCG-GFP. Specific immune responses were evaluated following intranasal immunisation of mice. Cellular uptake was approximately two-fold higher for chitosan-suspended BCG. A single dose of BCG-loaded microparticles or chitosan-suspended BCG by intranasal route improved Th1-type response compared with subcutaneous BCG. Chitosan-suspended BCG originated the highest mucosal response in the lungs by intranasal route. These positive results indicate that the proposed approach of whole live BCG microencapsulation in chitosan-alginate for intranasal immunisation was successful in allowing efficient interaction with APC, while improving the cellular immune response, which is of interest for local immunisation against tuberculosis.pt_PT
dc.description.versioninfo:eu-repo/semantics/acceptedVersionpt_PT
dc.identifier.citationCaetano LA, Figueiredo L, Almeida AJ, Gonçalves LMD. BCG-loaded chitosan microparticles: interaction with macrophages and preliminary in vivo studies. J Microencapsulation 2017:1-12. [Epub ahead of print]pt_PT
dc.identifier.doi10.1080/02652048.2017.1316325pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/7180
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relation.publisherversionhttp://www.tandfonline.com/doi/abs/10.1080/02652048.2017.1316325?journalCode=imnc20pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nd/4.0/pt_PT
dc.subjectBCG vaccinept_PT
dc.subjectChitosanpt_PT
dc.subjectMicroencapsulationpt_PT
dc.subjectMucosal vaccinationpt_PT
dc.titleBCG-loaded chitosan microparticles: interaction with macrophages and preliminary in vivo studiespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage12pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleJournal of Microencapsulationpt_PT
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT

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