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A microRNA atlas of CD4+ T cell subsets in experimental autoimmune encephalomyelitis identifies regulators of disease pathogenesis

2026-08Artigo científico Acesso aberto
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datacite.subject.sdg03:Saúde de Qualidade
dc.contributor.authorCunha, Carolina
dc.contributor.authorInácio, Daniel
dc.contributor.authorRomero, Paula Vargas
dc.contributor.authorPais, Ana Teresa
dc.contributor.authorPelicano, Catarina
dc.contributor.authorCosta, Marina
dc.contributor.authorMensurado, Sofia
dc.contributor.authorGonçalves-Sousa, Natacha
dc.contributor.authorPapotto, Pedro H.
dc.contributor.authorNeves, Daniel
dc.contributor.authorSobral, Daniel
dc.contributor.authorEnguita, Francisco
dc.contributor.authorSilva-Santos, Bruno
dc.contributor.authorGomes, Anita
dc.date.accessioned2026-08-27T09:08:09Z
dc.date.available2026-08-27T09:08:09Z
dc.date.issued2026-08
dc.descriptionCD4+ T cell RNA-seq data are available from the Sequence Read Archive (SRA) under the accession code PRJNA1067547 (https://www.ncbi.nlm.nih.gov/bioproject/PRJNA1067547). The raw flow cytometry data (FCS files) have been deposited in Zenodo and are available at https://doi.org/10.5281/zenodo.21341947. All other data that support the findings of this study are available in the article and Supplementary Information.
dc.descriptionThis work was funded by the European Research Council, by “la Caixa” Foundation, and by Fundação para a Ciência e Tecnologia. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
dc.description.abstractMicroRNAs (miRNAs) are key regulators of CD4+ T cell differentiation, but how they contribute to the course of an autoimmune disease in vivo remains poorly studied. Given the known roles in autoimmunity of pro-inflammatory T helper 1 (Th)1 and Th17 cells, and anti-inflammatory Foxp3+ regulatory cells, we established a triple-reporter mouse for Ifng, Il17, and Foxp3. We subjected it to experimental autoimmune encephalomyelitis (EAE) to characterize the miRNomes of the corresponding CD4+ T cell subsets. We identified 110 miRNAs differentially expressed between the pro-inflammatory (Th1 and Th17 cells) and the Treg cell subsets. Among these, we found novel functions for miR-122-5p and miR-1247 as regulators of Th17 cell proliferation and Th1 cell differentiation, thus impacting the course or severity of EAE, respectively. Importantly, their expression patterns suggest that miR-122-5p and miR-1247 act as peripheral brakes on CD4+ T cell pathogenicity that are subverted in the inflamed central nervous system.eng
dc.description.sponsorshipThis work was funded by the European Research Council (https://erc.europa.eu/homepage) (CoG_646701 to B.S.-S.), by “la Caixa” Foundation (https://lacaixafoundation.org/en/) (LCF/PR/HR24/00929 to B.S.S.); and by Fundação para a Ciência e Tecnologia (https://www.fct.pt) (SFRH/BD/145352/2019 PhD grant to D. I.). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
dc.identifier.citationCunha C, Inácio D, Romero PV, Pais AT, Pelicano C, Gomes AQ, et al. A microRNA atlas of CD4+ T cell subsets in experimental autoimmune encephalomyelitis identifies regulators of disease pathogenesis. PLoS Biol. 2026;24(8):e3003271.
dc.identifier.doi10.1371/journal.pbio.3003271
dc.identifier.issn1545-7885
dc.identifier.urihttp://hdl.handle.net/10400.21/23023
dc.language.isoeng
dc.peerreviewedyes
dc.publisherPublic Library of Science (PLoS)
dc.relation.hasversionhttps://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.3003271
dc.relation.ispartofPLOS Biology
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectEncephalomyelitis
dc.subjectCD4+ T cell subsets
dc.subjectmicroRNA atlas
dc.titleA microRNA atlas of CD4+ T cell subsets in experimental autoimmune encephalomyelitis identifies regulators of disease pathogenesiseng
dc.typejournal article
dcterms.referenceshttps://doi.org/10.5281/zenodo.21341947
dspace.entity.typePublication
oaire.citation.issue8
oaire.citation.startPagee3003271
oaire.citation.titlePLoS Biology
oaire.citation.volume24
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameGomes
person.givenNameAnita
person.identifier.ciencia-id4B10-E015-52B7
person.identifier.orcid0000-0002-3348-0448
person.identifier.ridC-3580-2014
person.identifier.scopus-author-id7202386033
relation.isAuthorOfPublication2b5a3f0a-29c2-4ecd-a83f-50d0b99a4875
relation.isAuthorOfPublication.latestForDiscovery2b5a3f0a-29c2-4ecd-a83f-50d0b99a4875

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