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Epigenetic and drug response modulation of epigalocaten-in-3-gallate in staphylococcus aureus with divergent resistance phenotypes

2023-03-04Journal article Open access
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dc.contributor.authorMira, Ana Rita
dc.contributor.authorZeferino, Ana Sofia
dc.contributor.authorInácio, Raquel
dc.contributor.authorDelgadinho, Mariana
dc.contributor.authorBrito, Miguel
dc.contributor.authorCalado, Cecília
dc.contributor.authorRibeiro, Edna
dc.date.accessioned2023-05-22T10:16:59Z
dc.date.available2023-05-22T10:16:59Z
dc.date.issued2023-03-04
dc.description.abstractHealthcare-associated methicillin-resistant Staphylococcus aureus infections represent extremely high morbidity and mortality rates worldwide. We aimed to assess the antimicrobial potential and synergistic effect between Epigalocatenin-3-gallate (EGCG) and different antibiotics in S. aureus strains with divergent resistance phenotypes. EGCG exposure effects in epigenetic and drug resistance key modulators were also evaluated. S. aureus strains (n = 32) were isolated from infected patients in a Lisbon hospital. The identification of the S. aureus resistance phenotype was performed through automatized methods. The antibiotic synergistic assay was performed through disk diffusion according to EUCAST guidelines with co-exposure to EGCG (250, 100, 50 and 25 µg/mL). The bacteria’s molecular profile was assessed through FTIR spectroscopy. The transcriptional expression of OrfX, SpdC and WalKR was performed by using qRT-PCR. FTIR-spectroscopy analysis enabled the clear discrimination of MRSA/MSSA strains and the EGCG exposure effect in the bacteria’s molecular profiles. Divergent resistant phenotypes were associated with divergent transcriptional expression of the epigenetic modulator OrfX, particularly in MRSA strains, as well as the key drug response modulators SpdC and WalKR. These results clearly demonstrate that EGCG exposure alters the expression patterns of key epigenetic and drug response genes with associated divergent-resistant profiles, which supports its potential for antimicrobial treatment and/or therapeutic adjuvant against antibiotic-resistant microorganisms.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMIRA, Ana Rita; [et al] – Epigenetic and drug response modulation of epigalocaten-in-3-gallate in staphylococcus aureus with divergent resistance phenotypes. Antibiotics. eISSN 2079-6382. Vol. 12, N.º 3 (2023), pp. 1-16.pt_PT
dc.identifier.doi10.3390/antibiotics12030519pt_PT
dc.identifier.eissn2079-6382
dc.identifier.urihttp://hdl.handle.net/10400.21/16092
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationIPL/2020/EpiResistanceSA_ESTeSL - Instituto Politécnico de Lisboapt_PT
dc.relationHealth and Technology Research Center
dc.relationHealth and Technology Research Center
dc.relation.publisherversionhttps://www.mdpi.com/2079-6382/12/3/519pt_PT
dc.subjectHealthcare-associated S. aureus infectionspt_PT
dc.subjectEpigallocatechin-3-gallate (EGCG)pt_PT
dc.subjectSynergistic effectspt_PT
dc.subjectDrug resistance phenotypespt_PT
dc.subjectEpigenetic modulatorpt_PT
dc.subjectDrug resistance modulatorspt_PT
dc.titleEpigenetic and drug response modulation of epigalocaten-in-3-gallate in staphylococcus aureus with divergent resistance phenotypespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleHealth and Technology Research Center
oaire.awardTitleHealth and Technology Research Center
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F05608%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F05608%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/DSAIPA%2FDS%2F0117%2F2020/PT
oaire.citation.endPage16pt_PT
oaire.citation.issue3pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleAntibioticspt_PT
oaire.citation.volume12pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream3599-PPCDT
person.familyNameNeves Delgadinho
person.familyNameBrito
person.familyNameCalado
person.givenNameMariana Isabel
person.givenNameMiguel
person.givenNameCecília
person.identifierCAJ-5082-2022
person.identifier130332
person.identifier.ciencia-id231E-02E3-D9A9
person.identifier.ciencia-id231F-F341-7E93
person.identifier.ciencia-id9418-E320-3177
person.identifier.orcid0000-0003-0586-9154
person.identifier.orcid0000-0001-6394-658X
person.identifier.orcid0000-0002-5264-9755
person.identifier.ridA-7970-2016
person.identifier.ridE-2102-2014
person.identifier.scopus-author-id35224551000
person.identifier.scopus-author-id6603163260
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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