Browsing by Issue Date, starting with "2023-03-04"
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- Energy-efficient and real-time wearable for wellbeing-monitoring IoT system based on SoC-FPGAPublication . Frutuoso, Maria Inês; Cláudio de Campos Neto, Horácio; Véstias, Mário; Duarte, Rui PolicarpoWearable devices used for personal monitoring applications have been improved over the last decades. However, these devices are limited in terms of size, processing capability and power consumption. This paper proposes an efficient hardware/software embedded system for monitoring bio-signals in real time, including a heart rate calculator using PPG and an emotion classifier from EEG. The system is suitable for outpatient clinic applications requiring data transfers to external medical staff. The proposed solution contributes with an effective alternative to the traditional approach of processing bio-signals offline by proposing a SoC-FPGA based system that is able to fully process the signals locally at the node. Two sub-systems were developed targeting a Zynq 7010 device and integrating custom hardware IP cores that accelerate the processing of the most complex tasks. The PPG sub-system implements an autocorrelation peak detection algorithm to calculate heart rate values. The EEG sub-system consists of a KNN emotion classifier of preprocessed EEG features. This work overcomes the processing limitations of microcontrollers and general-purpose units, presenting a scalable and autonomous wearable solution with high processing capability and real-time response.
- Epigenetic and drug response modulation of epigalocaten-in-3-gallate in staphylococcus aureus with divergent resistance phenotypesPublication . Mira, Ana Rita; Zeferino, Ana Sofia; Inácio, Raquel; Delgadinho, Mariana; Brito, Miguel; Calado, Cecília; Ribeiro, EdnaHealthcare-associated methicillin-resistant Staphylococcus aureus infections represent extremely high morbidity and mortality rates worldwide. We aimed to assess the antimicrobial potential and synergistic effect between Epigalocatenin-3-gallate (EGCG) and different antibiotics in S. aureus strains with divergent resistance phenotypes. EGCG exposure effects in epigenetic and drug resistance key modulators were also evaluated. S. aureus strains (n = 32) were isolated from infected patients in a Lisbon hospital. The identification of the S. aureus resistance phenotype was performed through automatized methods. The antibiotic synergistic assay was performed through disk diffusion according to EUCAST guidelines with co-exposure to EGCG (250, 100, 50 and 25 µg/mL). The bacteria’s molecular profile was assessed through FTIR spectroscopy. The transcriptional expression of OrfX, SpdC and WalKR was performed by using qRT-PCR. FTIR-spectroscopy analysis enabled the clear discrimination of MRSA/MSSA strains and the EGCG exposure effect in the bacteria’s molecular profiles. Divergent resistant phenotypes were associated with divergent transcriptional expression of the epigenetic modulator OrfX, particularly in MRSA strains, as well as the key drug response modulators SpdC and WalKR. These results clearly demonstrate that EGCG exposure alters the expression patterns of key epigenetic and drug response genes with associated divergent-resistant profiles, which supports its potential for antimicrobial treatment and/or therapeutic adjuvant against antibiotic-resistant microorganisms.