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The interplay between HPV, other sexually transmissible infections and genital microbiome on cervical microenvironment (MicroCervixHPV study)

2024-03Journal article Open access
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dc.contributor.authorGonçalves-Nobre, J. Guilherme
dc.contributor.authorMatos, Andreia
dc.contributor.authorCarreira, Mariana
dc.contributor.authorSantos, Ana Carolina
dc.contributor.authorVeiga, Luisa
dc.contributor.authorGinete, Catarina
dc.contributor.authorBrito, Miguel
dc.contributor.authorPires, Marina
dc.contributor.authorPereira, Hermínia
dc.contributor.authorCardoso, Carlos
dc.contributor.authorBicho, Manuel
dc.contributor.authorBicho, Maria Clara
dc.date.accessioned2024-04-01T10:44:25Z
dc.date.available2024-04-01T10:44:25Z
dc.date.issued2024-03
dc.descriptionThe author(s) declare financial support was received for the research, authorship, and/or publication of this article. The writing of this manuscript received the support of Fundação para a Ciência e a Tecnologia, Grant number UIDB/04295/2020, and doctoral scholarships (SFRH/BD/132900/2017). The authors acknowledge financial support from the Instituto de Investigação Científica Bento da Rocha Cabral, Clinical Analysis Laboratory Joaquim Chaves Saúde, Instituto de Saúde Ambiental, Faculdade de Medicina da Universidade de Lisboa and Escola Superior de Tecnologia de Saúde de Lisboa, Instituto Politécnico de Lisboa.pt_PT
dc.description.abstractBackground: The importance of Cervicovaginal Microbiota in protecting against infections (such as HPV) is already well established, namely through Lactobacillus spp., as well as the mechanism through which HPV leads to Cervical Neoplasia. However, it is not possible to classify HPV as a complete carcinogen. Thus, the importance of exploring Cervicovaginal dysbiosis to decipher this interaction with HPV takes on greater relevance. The main objectives of this study were: 1) Comparison of the MCV composition of women with or without HPV and women with ASCUS or LSIL; 2) Characterization of cytokines present in the vaginal microenvironment; 3) Evaluation of the blood count ratios as prognostic systemic inflammatory biomarkers; 4) Correlation between MCV, HPV serotypes, and cytokines. Methods: This was a retrospective, observational, multicenter, cross-sectional study. CVM analysis was performed by isolation RNA and sequencing on a NGS platform. Cytokine concentrations of CVM were obtained through the Multiplex platform. Statistical analysis was performed in SPSS v 26.0. An α of 0.05 was considered statistically significant. Results: Highlighting the core of the study, CVM types of CST I and CST IV were found to influence the emergence of cervical lesions. The neutrophil-to-lymphocyte ratio was found to impact the prognosis of ASCUS. Within CVM, Lactobacillus prevents the growth of other CST IV species, while the latter express symbiotic relationships with each other and show affinity for specific HPV serotypes. At last, RANTES chemokine is significantly elevated in cervicovaginal infections. Conclusion: The importance of using vaginal cytokine profiles and CVM is highlighted in the hypothesis of prevention of Cervical Neoplasia development, as well as in its use as a prognostic biomarker. Taken together, these insights are one step closer to personalized medicine.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationGonçalves-Nobre JG, Matos A, Carreira M, Veiga LC, Ginete C, Brito M, et al. The interplay between HPV, other sexually transmissible infections and genital microbiome on cervical microenvironment (MicroCervixHPV study). Front Cell Infect Microbiol. 2024;13:1251913.pt_PT
dc.identifier.doi10.3389/fcimb.2023.1251913pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/17233
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherFrontiers Mediapt_PT
dc.relation.publisherversionhttps://www.frontiersin.org/articles/10.3389/fcimb.2023.1251913/fullpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectHPVpt_PT
dc.subjectBlood count ratiospt_PT
dc.subjectCervical intra-epithelial neoplasiapt_PT
dc.subjectCervical neoplasiapt_PT
dc.subjectCervicovaginal microbiotapt_PT
dc.subjectCytokinespt_PT
dc.titleThe interplay between HPV, other sexually transmissible infections and genital microbiome on cervical microenvironment (MicroCervixHPV study)pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.startPage1251913pt_PT
oaire.citation.titleFrontiers in Cellular and Infection Microbiologypt_PT
oaire.citation.volume13pt_PT
person.familyNameVeiga
person.familyNameHonrado Ginete
person.familyNameBrito
person.givenNameLuisa
person.givenNameAna Catarina
person.givenNameMiguel
person.identifier.ciencia-id9413-918D-DB0E
person.identifier.ciencia-id8715-F62E-1E0F
person.identifier.ciencia-id231F-F341-7E93
person.identifier.orcid0000-0003-1153-8343
person.identifier.orcid0000-0002-2334-782X
person.identifier.orcid0000-0001-6394-658X
person.identifier.ridL-2730-2013
person.identifier.ridA-7970-2016
person.identifier.scopus-author-id8318978600
person.identifier.scopus-author-id35224551000
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationaac1914a-275f-4be2-819d-ab41d356b45a
relation.isAuthorOfPublicationdfb2fbba-17ff-42fb-905a-fcfc8f326e1c
relation.isAuthorOfPublication4252d8e0-800c-4d67-8b13-0b711d860669
relation.isAuthorOfPublication.latestForDiscoveryaac1914a-275f-4be2-819d-ab41d356b45a

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