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TBCCD1, a new centrosomal protein, is required for centrosome and Golgi apparatus positioning

2010-03Journal article Open access
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dc.contributor.authorGonçalves, João
dc.contributor.authorNolasco, Sofia
dc.contributor.authorNascimento, Rute
dc.contributor.authorLopez Fanarraga, Mónica
dc.contributor.authorZabala, Juan Carlos
dc.contributor.authorSoares, Helena
dc.date.accessioned2013-06-26T14:22:49Z
dc.date.available2013-06-26T14:22:49Z
dc.date.issued2010-03
dc.description.abstractIn animal cells the centrosome is positioned at the cell centre in close association with the nucleus. The mechanisms responsible for this are not completely understood. Here, we report the first characterization of human TBCC-domain containing 1 (TBCCD1), a protein related to tubulin cofactor C. TBCCD1 localizes at the centrosome and at the spindle midzone, midbody and basal bodies of primary and motile cilia. Knockdown of TBCCD1 in RPE-1 cells caused the dissociation of the centrosome from the nucleus and disorganization of the Golgi apparatus. TBCCD1-depleted cells are larger, less efficient in primary cilia assembly and their migration is slower in wound-healing assays. However, the major microtubule-nucleating activity of the centrosome is not affected by TBCCD1 silencing. We propose that TBCCD1 is a key regulator of centrosome positioning and consequently of internal cell organization.por
dc.identifier.citationGonçalves J, Nolasco S, Nascimento R, Lopez Fanarraga M, Zabala JC, Soares H. TBCCD1, a new centrosomal protein, is required for centrosome and Golgi apparatus positioning. EMBO Rep. 2010;11(3):194-200.por
dc.identifier.issn1469-3178
dc.identifier.urihttp://hdl.handle.net/10400.21/2582
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherEuropean Molecular Biology Organizationpor
dc.relation.publisherversionhttp://www.nature.com/embor/journal/v11/n3/full/embor20105.htmlpor
dc.subjectCell migrationpor
dc.subjectCentrosomepor
dc.subjectGolgi apparatuspor
dc.subjectPrimary ciliapor
dc.subjectTBCCD1por
dc.subjectCarrier proteinspor
dc.subjectCell nucleuspor
dc.subjectGene silencingpor
dc.subjectGenetic complementation testpor
dc.subjectMitotic spindle apparatuspor
dc.subjectMolecular chaperonespor
dc.subjectRNA interferencepor
dc.titleTBCCD1, a new centrosomal protein, is required for centrosome and Golgi apparatus positioningpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage200por
oaire.citation.startPage194por
oaire.citation.titleEMBO Reportspor
oaire.citation.volume11por
rcaap.rightsopenAccesspor
rcaap.typearticlepor

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