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Fat intake interacts with polymorphisms of Caspase9, FasLigand and PPARgamma apoptotic genes in modulating Crohn's disease activity

2010-12Journal article Open access
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dc.contributor.authorFerreira, Paula
dc.contributor.authorCravo, Marília
dc.contributor.authorGuerreiro, Catarina Sousa
dc.contributor.authorTavares, Lourdes
dc.contributor.authorSantos, Paula Moura dos
dc.contributor.authorBrito, Miguel
dc.date.accessioned2014-01-02T16:04:12Z
dc.date.available2014-01-02T16:04:12Z
dc.date.issued2010-12
dc.description.abstractBackground & aims: Crohn’s disease (CD) is a multifactorial disease where resistance to apoptosis is one major defect. Also, dietary fat intake has been shown to modulate disease activity. We aimed to explore the interaction between four single nucleotide polymorphisms (SNPs) in apoptotic genes and dietary fat intake in modulating disease activity in CD patients. Methods: Polymerase Chain Reaction (PCR) and Restriction Fragment Length Polymorphism (RFLP) techniques were used to analyze Caspase9þ93C/T, FasLigand-843C/T, Peroxisome Proliferator-Activated Receptor gammaþ161C/T and Peroxisome Proliferator-Activated Receptor gamma Pro12Ala SNPs in 99 patients with CD and 116 healthy controls. Interactions between SNPs and fat intake in modulating disease activity were analyzed using regression analysis. Results: None of the polymorphisms analyzed influenced disease susceptibility and/or activity, but a high intake of total, saturated and monounsaturated fats and a higher ratio of n-6/n-3 polyunsaturated fatty acids (PUFA), was associated with a more active phenotype (p < 0.05). We observed that the detrimental effect of a high intake of total and trans fat was more marked in wild type carriers of the Caspase9þ93C/T polymorphism [O.R (95%CI) 4.64 (1.27e16.89) and O.R (95%CI) 4.84 (1.34e17.50)]. In the Peroxisome Proliferator-Activated Receptor gamma Pro12Ala SNP, we also observed that a high intake of saturated and monounsaturated fat was associated to a more active disease in wild type carriers [OR (95%CI) 4.21 (1.33e13.26) and 4.37 (1.52e12.51)]. Finally, a high intake of n-6 PUFA was associated with a more active disease in wild type carriers for the FasLigand-843C/T polymorphism [O.R (95%CI) 5.15 (1.07e24.74)]. Conclusions: To our knowledge, this is the first study to disclose a synergism between fat intake and SNPs in apoptotic genes in modulating disease activity in CD patients.por
dc.identifier.citationFerreira P, Cravo M, Guerreiro CS, Tavares L, Santos PM, Brito M. Fat intake interacts with polymorphisms of Caspase9, FasLigand and PPARgamma apoptotic genes in modulating Crohn's disease activity. Clin Nutr. 2010;29(6):819-23.por
dc.identifier.issn1532-1983
dc.identifier.urihttp://hdl.handle.net/10400.21/3051
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relation.publisherversionhttp://download.journals.elsevierhealth.com/pdfs/journals/0261-5614/PIIS026156141000110X.pdfpor
dc.subjectCase-control studiespor
dc.subjectCaspase 9por
dc.subjectCrohn diseasepor
dc.subjectDietpor
dc.subjectDietary fatspor
dc.subjectFas ligand proteinpor
dc.subjectFatty acidspor
dc.subjectGenetic predisposition to diseasepor
dc.subjectPPAR gammpor
dc.subjectPolymorphism, Single nucleotidepor
dc.subjectRegression analysispor
dc.titleFat intake interacts with polymorphisms of Caspase9, FasLigand and PPARgamma apoptotic genes in modulating Crohn's disease activitypor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage823por
oaire.citation.startPage819por
oaire.citation.titleClinical Nutritionpor
oaire.citation.volume29por
person.familyNameBrito
person.givenNameMiguel
person.identifier.ciencia-id231F-F341-7E93
person.identifier.orcid0000-0001-6394-658X
person.identifier.ridA-7970-2016
person.identifier.scopus-author-id35224551000
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication4252d8e0-800c-4d67-8b13-0b711d860669
relation.isAuthorOfPublication.latestForDiscovery4252d8e0-800c-4d67-8b13-0b711d860669

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