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Epigenetic and drug response modulation of Epigalocaten-in-3-gallate in Staphylococcus aureus with divergent resistance phenotypes

2023-03Journal article Open access
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dc.contributor.authorMira, Ana Rita
dc.contributor.authorZeferino, Ana Sofia
dc.contributor.authorInácio, Raquel
dc.contributor.authorDelgadinho, Mariana
dc.contributor.authorBrito, Miguel
dc.contributor.authorCalado, Cecília R. C.
dc.contributor.authorRibeiro, Edna
dc.date.accessioned2023-04-21T17:55:26Z
dc.date.available2023-04-21T17:55:26Z
dc.date.issued2023-03
dc.descriptionProjeto “Resistance modulation and epigenetic divergence in resistant phenotypic profiles of Staphylococcus aureus” (IPL/2020/ EpiResistanceSA _ESTeSL).pt_PT
dc.descriptionFCT_UIDB/05608/2020. FCT_UIDP/05608/2020.pt_PT
dc.description.abstractHealthcare-associated methicillin-resistant Staphylococcus aureus infections represent extremely high morbidity and mortality rates worldwide. We aimed to assess the antimicrobial potential and synergistic effect between Epigalocatenin-3-gallate (EGCG) and different antibiotics in S. aureus strains with divergent resistance phenotypes. EGCG exposure effects in epigenetic and drug resistance key modulators were also evaluated. S. aureus strains (n = 32) were isolated from infected patients in a Lisbon hospital. The identification of the S. aureus resistance phenotype was performed through automatized methods. The antibiotic synergistic assay was performed through disk diffusion according to EUCAST guidelines with co-exposure to EGCG (250, 100, 50, and 25 µg/mL). The bacteria's molecular profile was assessed through FTIR spectroscopy. The transcriptional expression of OrfX, SpdC, and WalKR was performed by using qRT-PCR. FTIR-spectroscopy analysis enabled the clear discrimination of MRSA/MSSA strains and the EGCG exposure effect in the bacteria's molecular profiles. Divergent resistant phenotypes were associated with divergent transcriptional expression of the epigenetic modulator OrfX, particularly in MRSA strains, as well as the key drug response modulators SpdC and WalKR. These results clearly demonstrate that EGCG exposure alters the expression patterns of key epigenetic and drug response genes with associated divergent-resistant profiles, which supports its potential for antimicrobial treatment and/or therapeutic adjuvant against antibiotic-resistant microorganisms.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMira AR, Zeferino AS, Inácio R, Delgadinho M, Brito M, Calado CR, Ribeiro E. Epigenetic and drug response modulation of Epigalocaten-in-3-gallate in Staphylococcus aureus with divergent resistance phenotypes. Antibiotics. 2023;12(3):519.pt_PT
dc.identifier.doi10.3390/antibiotics12030519pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/15932
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationIPL/2020/ EpiResistanceSA _ESTeSLpt_PT
dc.relation.publisherversionhttps://www.mdpi.com/2079-6382/12/3/519pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectDrug resistance modulatorspt_PT
dc.subjectDrug resistance phenotypespt_PT
dc.subjectEpigallocatechin-3-gallatept_PT
dc.subjectEGCGpt_PT
dc.subjectEpigenetic modulatorpt_PT
dc.subjectHealthcare-associated S. aureus infectionspt_PT
dc.subjectSynergistic effectspt_PT
dc.subjectIPL/2020/ EpiResistanceSA _ESTeSLpt_PT
dc.subjectFCT_UIDB/05608/2020pt_PT
dc.subjectFCT_UIDP/05608/2020pt_PT
dc.titleEpigenetic and drug response modulation of Epigalocaten-in-3-gallate in Staphylococcus aureus with divergent resistance phenotypespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue3pt_PT
oaire.citation.startPage519pt_PT
oaire.citation.titleAntibioticspt_PT
oaire.citation.volume12pt_PT
person.familyNameNeves Delgadinho
person.familyNameBrito
person.familyNameRibeiro
person.givenNameMariana Isabel
person.givenNameMiguel
person.givenNameEdna
person.identifierCAJ-5082-2022
person.identifier.ciencia-id231E-02E3-D9A9
person.identifier.ciencia-id231F-F341-7E93
person.identifier.ciencia-idC414-CDF2-D35A
person.identifier.orcid0000-0003-0586-9154
person.identifier.orcid0000-0001-6394-658X
person.identifier.orcid0000-0003-1316-7750
person.identifier.ridA-7970-2016
person.identifier.scopus-author-id35224551000
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationca55aab6-9a58-4f79-ab79-20513414099f
relation.isAuthorOfPublication4252d8e0-800c-4d67-8b13-0b711d860669
relation.isAuthorOfPublicationa571bf34-bcda-49ca-b5cb-4cdecbb3d9c7
relation.isAuthorOfPublication.latestForDiscoverya571bf34-bcda-49ca-b5cb-4cdecbb3d9c7

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