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Measuring DNA modifications with the comet assay: a compendium of protocols

2023-03Journal article Open access
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dc.contributor.authorCollins, Andrew
dc.contributor.authorMøller, Peter
dc.contributor.authorGajski, Goran
dc.contributor.authorVodenková, Soňa
dc.contributor.authorAbdulwahed, Abdulhadi
dc.contributor.authorAnderson, Diana
dc.contributor.authorBankoglu, Ezgi Eyluel
dc.contributor.authorBonassi, Stefano
dc.contributor.authorBoutet-Robinet, Elisa
dc.contributor.authorBrunborg, Gunnar
dc.contributor.authorChao, Christy
dc.contributor.authorCooke, Marcus S.
dc.contributor.authorCosta, Carla
dc.contributor.authorCosta, Solange
dc.contributor.authorDhawan, Alok
dc.contributor.authorde Lapuente, Joaquin
dc.contributor.authorBo’, Cristian Del
dc.contributor.authorDubus, Julien
dc.contributor.authorDusinska, Maria
dc.contributor.authorDuthie, Susan J.
dc.contributor.authorYamani, Naouale El
dc.contributor.authorEngelward, Bevin
dc.contributor.authorGaivão, Isabel
dc.contributor.authorGiovannelli, Lisa
dc.contributor.authorGodschalk, Roger
dc.contributor.authorGuilherme, Sofia
dc.contributor.authorGutzkow, Kristine B.
dc.contributor.authorHabas, Khaled
dc.contributor.authorHernández, Alba
dc.contributor.authorHerrero, Oscar
dc.contributor.authorIsidori, Marina
dc.contributor.authorJha, Awadhesh N.
dc.contributor.authorKnasmüller, Siegfried
dc.contributor.authorKooter, Ingeborg M.
dc.contributor.authorKoppen, Gudrun
dc.contributor.authorKruszewski, Marcin
dc.contributor.authorLadeira, Carina
dc.contributor.authorLaffon, Blanca
dc.contributor.authorLarramendy, Marcelo
dc.contributor.authorHégarat, Ludovic Le
dc.contributor.authorLewies, Angélique
dc.contributor.authorLewinska, Anna
dc.contributor.authorLiwszyc, Guillermo E.
dc.contributor.authorde Cerain, Adela López
dc.contributor.authorManjanatha, Mugimane
dc.contributor.authorMarcos, Ricard
dc.contributor.authorMilić, Mirta
dc.contributor.authorde Andrade, Vanessa Moraes
dc.contributor.authorMoretti, Massimo
dc.contributor.authorMuruzabal, Damian
dc.contributor.authorNovak, Matjaž
dc.contributor.authorOliveira, Rui
dc.contributor.authorOlsen, Ann-Karin
dc.contributor.authorOwiti, Norah
dc.contributor.authorPacheco, Mário
dc.contributor.authorPandey, Alok K.
dc.contributor.authorPfuhler, Stefan
dc.contributor.authorPourrut, Bertrand
dc.contributor.authorReisinger, Kerstin
dc.contributor.authorRojas, Emilio
dc.contributor.authorRundén-Pran, Elise
dc.contributor.authorSanz-Serrano, Julen
dc.contributor.authorShaposhnikov, Sergey
dc.contributor.authorSipinen, Ville
dc.contributor.authorSmeets, Karen
dc.contributor.authorStopper, Helga
dc.contributor.authorTeixeira, João Paulo
dc.contributor.authorValdiglesias, Vanessa
dc.contributor.authorValverde, Mahara
dc.contributor.authorvan Acker, Frederique
dc.contributor.authorvan Schooten, Frederik-Jan
dc.contributor.authorVasquez, Marie
dc.contributor.authorWentzel, Johannes F.
dc.contributor.authorWnuk, Maciej
dc.contributor.authorWouters, Annelies
dc.contributor.authorŽegura, Bojana
dc.contributor.authorZikmund, Tomas
dc.contributor.authorLangie, Sabine A. S.
dc.contributor.authorAzqueta, Amaya
dc.date.accessioned2023-02-02T17:06:18Z
dc.date.embargo2025-02-02
dc.date.issued2023-03
dc.description.abstractThe comet assay is a versatile method to detect nuclear DNA damage in individual eukaryotic cells, from yeast to humans. The types of damage detected encompass DNA strand breaks and alkali-labile sites (e.g., apurinic/apyrimidinic sites), alkylated and oxidized nucleobases, DNA-DNA crosslinks, UV-induced cyclobutane pyrimidine dimers, some chemically induced DNA adducts. Depending on the specimen type, there are important modifications to the comet assay protocol to avoid the formation of additional DNA damage during the processing of samples and to ensure sufficient sensitivity to detect differences in damage levels between sample groups. Various applications of the comet assay have been validated by research groups in academia, industry, and regulatory agencies, and its strengths are highlighted by the adoption of the comet assay as an in vivo test for genotoxicity in animal organs by the Organisation for Economic Co-operation and Development. The present document includes a series of consensus protocols that describe the application of the comet assay to a wide variety of cell types, species, and types of DNA damage, thereby demonstrating its versatility.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCollins A, Møller P, Gajski G, Vodenková S, Abdulwahed A, Ladeira C, et al. Measuring DNA modifications with the comet assay: a compendium of protocols. Nat Protoc. 2023;18(3):929-89.pt_PT
dc.identifier.doi10.1038/s41596-022-00754-ypt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/15472
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringer Naturept_PT
dc.relation.publisherversionhttps://www.nature.com/articles/s41596-022-00754-ypt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectComet assaypt_PT
dc.subjectDNA modificationspt_PT
dc.subjectProtocolpt_PT
dc.titleMeasuring DNA modifications with the comet assay: a compendium of protocolspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage989pt_PT
oaire.citation.issue3pt_PT
oaire.citation.startPage929pt_PT
oaire.citation.titleNature Protocolspt_PT
oaire.citation.volume18pt_PT
person.familyNameLadeira
person.givenNameCarina
person.identifier144237
person.identifier.ciencia-id801C-1BBA-1D9E
person.identifier.orcid0000-0001-5588-0074
person.identifier.ridJ-2572-2012
person.identifier.scopus-author-id36463788000
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication1aef4b60-4197-436b-84ab-80d31cbaed33
relation.isAuthorOfPublication.latestForDiscovery1aef4b60-4197-436b-84ab-80d31cbaed33

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