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Tetrahymena cilia cap is built in a multi-step process: a study by atomic force microscopy

2017-10Journal article Restricted access
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dc.contributor.authorSeixas, Cecília
dc.contributor.authorGonçalves, João
dc.contributor.authorMelo, Luís Viseu
dc.contributor.authorSoares, Helena
dc.date.accessioned2017-11-06T11:17:14Z
dc.date.available2017-11-06T11:17:14Z
dc.date.issued2017-10
dc.description.abstractCilia are complex and dynamic organelles that have motility and sensory functions. Defects in cilia biogenesis and function are at the origin of human ciliopathies. In motile cilia, a basal body organizes the axoneme composed of nine microtubule doublets surrounding a central pair of singlet microtubules. The distal ends of axonemal microtubules are attached to the membrane by microtubule-capping structures. Little is known about the early steps of cilium assembly. Although cilia grow and resorb from their distal tips, it remains poorly understood where and when the components of the caps are first assembled. By using Atomic Force Microscopy in tapping mode, with resolution at the nanometer range and with minimum sample manipulation, we show that Tetrahymena cilia assembly requires transient assembly of structures, composed of three components that are placed asymmetrically on an early elongating axoneme. In small uncapped axonemes, the microtubule central pair was never observed. Additionally, we show that cilia cap assembly is a multi-step process in which structures of different sizes and shapes are put together in close proximity before the axoneme appears capped. We propose that the cap modifies the axoneme microtubule rate of polymerization and present a model for Tetrahymena cilia cap assembly.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSeixas C, Gonçalves J, Melo LV, Soares H. Tetrahymena cilia cap is built in a multi-step process: a study by atomic force microscopy. Protist. 2017;168(6):697-717.pt_PT
dc.identifier.doi10.1016/j.protis.2017.10.001pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/7457
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S1434461017300913pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectTetrahymenapt_PT
dc.subjectCilia assemblypt_PT
dc.subjectCap assemblypt_PT
dc.subjectAFMpt_PT
dc.subjectRe-ciliationpt_PT
dc.titleTetrahymena cilia cap is built in a multi-step process: a study by atomic force microscopypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage717pt_PT
oaire.citation.issue6pt_PT
oaire.citation.startPage697pt_PT
oaire.citation.titleProtistpt_PT
oaire.citation.volume168pt_PT
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT

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