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Microbial gut evaluation in an angolan paediatric population with sickle cell disease

2022-09Journal article Open access
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dc.contributor.authorDelgadinho, Mariana
dc.contributor.authorGinete, Catarina
dc.contributor.authorSantos, Brígida
dc.contributor.authorMendes, Joana
dc.contributor.authorMiranda, Armandina
dc.contributor.authorVasconcelos, Jocelyne
dc.contributor.authorBrito, Miguel
dc.date.accessioned2022-10-17T12:17:16Z
dc.date.available2022-10-17T12:17:16Z
dc.date.issued2022-09
dc.descriptionFCT/Aga Khan (project no. 330842553).pt_PT
dc.descriptionFCT_UIDB/05608/2020. FCT_UIDP/05608/2020.pt_PT
dc.description.abstractSickle cell disease (SCD) is one of the most common genetic conditions worldwide. It can contribute to up to 90% of under-5 mortality in sub-Saharan Africa. Clinical manifestations are very heterogeneous, and the intestinal microbiome appears to be crucial in the modulation of inflammation, cell adhesion, and induction of aged neutrophils, the main interveners of recurrent vaso-occlusive crisis. Enterocyte injury, increased permeability, altered microbial composition, and bacterial overgrowth have all been documented as microbial and pathophysiologic changes in the gut microbiome of SCD patients in recent studies. Our aim was to sequence the bacterial 16S rRNA gene in order to characterize the gut microbiome of Angolan children with SCA and healthy siblings as a control. A total of 72 stool samples were obtained from children between 3 and 14 years old. Our data showed that the two groups exhibit some notable differences in microbiota relative abundance at different classification levels. Children with SCA have a higher number of the phylum Actinobacteria. As for the genus level, Clostridium cluster XI bacteria was more prevalent in the SCA children, whereas the siblings had a higher abundance of Blautia, Aestuariispira, Campylobacter, Helicobacter, Polaribacter, and Anaerorhabdus. In this study, we have presented the first microbiota analysis in an Angolan paediatric population with SCD and provided a detailed view of the microbial differences between patients and healthy controls. There is still much to learn before fully relying on the therapeutic approaches for gut modulation, which is why more research in this field is crucial to making this a reality.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationDelgadinho M, Ginete C, Santos B, Mendes J, Miranda A, Brito M, et al. Microbial gut evaluation in an angolan paediatric population with sickle cell disease. J Cell Mol Med. 2022;26(21):5360-8.pt_PT
dc.identifier.doi10.1111/jcmm.17402pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/15033
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWileypt_PT
dc.relationFCT/Aga Khan (project no. 330842553)pt_PT
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/doi/10.1111/jcmm.17402pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectSickle cell diseasept_PT
dc.subjectFetal haemoglobinpt_PT
dc.subjectMicrobiomept_PT
dc.subject16S rRNApt_PT
dc.subjectChildrenpt_PT
dc.subjectAngolapt_PT
dc.subjectFCT_Aga Khan (project no. 330842553)pt_PT
dc.subjectFCT_UIDB/05608/2020pt_PT
dc.subjectFCT_UIDP/05608/2020pt_PT
dc.titleMicrobial gut evaluation in an angolan paediatric population with sickle cell diseasept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage5368pt_PT
oaire.citation.issue21pt_PT
oaire.citation.startPage5360pt_PT
oaire.citation.titleJournal of Cellular and Molecular Medicinept_PT
oaire.citation.volume26pt_PT
person.familyNameNeves Delgadinho
person.familyNameHonrado Ginete
person.familyNameBrito
person.givenNameMariana Isabel
person.givenNameAna Catarina
person.givenNameMiguel
person.identifierCAJ-5082-2022
person.identifier.ciencia-id231E-02E3-D9A9
person.identifier.ciencia-id8715-F62E-1E0F
person.identifier.ciencia-id231F-F341-7E93
person.identifier.orcid0000-0003-0586-9154
person.identifier.orcid0000-0002-2334-782X
person.identifier.orcid0000-0001-6394-658X
person.identifier.ridA-7970-2016
person.identifier.scopus-author-id35224551000
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationca55aab6-9a58-4f79-ab79-20513414099f
relation.isAuthorOfPublicationdfb2fbba-17ff-42fb-905a-fcfc8f326e1c
relation.isAuthorOfPublication4252d8e0-800c-4d67-8b13-0b711d860669
relation.isAuthorOfPublication.latestForDiscoveryca55aab6-9a58-4f79-ab79-20513414099f

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