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Preclinical study in vivo for new pharmacological approaches in inflammatory bowel disease: a systematic review of chronic model of TNBS-induced colitis

2019-10Journal article Open access
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dc.contributor.authorSilva, Inês
dc.contributor.authorPinto, Rui
dc.contributor.authorMateus, Vanessa
dc.date.accessioned2019-10-01T13:53:07Z
dc.date.available2019-10-01T13:53:07Z
dc.date.issued2019-10
dc.descriptionIPL_IDI&CA_COLITIS/2019pt_PT
dc.description.abstractThe preclinical studies in vivo provide means of characterizing physiologic interactions when our understanding of such processes is insufficient to allow replacement with in vitro systems and play a pivotal role in the development of a novel therapeutic drug cure. Chemically induced colitis models are relatively easy and rapid to develop. The 2,4,6-trinitrobenzenesulfonic acid (TNBS) colitis model is one of the main models in the experimental studies of inflammatory bowel disease (IBD) since inflammation induced by TNBS mimics several features of Crohn’s disease. This review aims to summarize the existing literature and discuss different protocols for the induction of chronic model of TNBS-induced colitis. We searched MEDLINE via Pubmed platform for studies published through December 2018, using MeSH terms (Crohn Disease.kw) OR (Inflammatory Bowel Diseases.kw) OR (Colitis, Ulcerative.kw) AND (trinitrobenzenesulfonic acid.kw) AND (disease models, animal.kw) AND (mice.all). The inclusion criteria were original articles, preclinical studies in vivo using mice, a chronic model of colitis, and TNBS as the inducer of colitis and articles published in English. Chronic TNBS-induced colitis is made with multiple TNBS intrarectal administrations in an average dose of 1.2 mg using a volume lower than 150 μL in 50% ethanol. The strains mostly used are Balb/c and C57BL/6 with 5–6 weeks. To characterize the preclinical model the parameters more used include body weight, stool consistency and morbidity, inflammatory biomarkers like interferon (IFN)-γ, myeloperoxidase (MPO), tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-10, presence of ulcers, thickness or hyperemia in the colon, and histological evaluation of the inflammation. Experimental chronic colitis is induced by multiple rectal instillations of TNBS increasing doses in ethanol using Balb/c and C57BL/6 mice.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSilva I, Pinto R, Mateus V. Preclinical study in vivo for new pharmacological approaches in inflammatory bowel disease: a systematic review of chronic model of TNBS-induced colitis. J Clin Med. 2019;8(10):1574.pt_PT
dc.identifier.doi10.3390/jcm8101574pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/10535
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationIPL_IDI&CA_COLITIS/2019pt_PT
dc.relation.publisherversionhttps://www.mdpi.com/2077-0383/8/10/1574pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectInflammatory bowel diseasept_PT
dc.subjectExperimental colitispt_PT
dc.subjectChronic modelspt_PT
dc.subjectTrinitrobenzene sulfonic acid-induced colitispt_PT
dc.subjectTNBS-induced colitispt_PT
dc.subjectIDI&CA_COLITIS/2019pt_PT
dc.titlePreclinical study in vivo for new pharmacological approaches in inflammatory bowel disease: a systematic review of chronic model of TNBS-induced colitispt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue10pt_PT
oaire.citation.startPage1574pt_PT
oaire.citation.titleJournal of Clinical Medicinept_PT
oaire.citation.volume8pt_PT
person.familyNameda Silva
person.familyNamePinho Mateus
person.givenNameInês Filipa Janeiro da Silva
person.givenNameVanessa Alexandra
person.identifier.ciencia-idC010-323F-3266
person.identifier.ciencia-id5A12-571D-AD6A
person.identifier.orcid0000-0001-7049-2512
person.identifier.orcid0000-0002-3204-3772
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication995e1831-ff5b-49e7-a6e3-8bc692212204
relation.isAuthorOfPublication406041a5-682c-4f94-a4e2-ddbfc541313c
relation.isAuthorOfPublication.latestForDiscovery995e1831-ff5b-49e7-a6e3-8bc692212204

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