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Authors
Advisor(s)
Abstract(s)
A infeção pelo HPV é uma das infeções de transmissão sexual mais comuns a nível mundial e os HPV de alto risco 16/18 são responsáveis por cerca de 70% dos casos de cancro do colo do útero. Para além desta patologia, também pode causar outros cancros anogenitais, cancro da cabeça e pescoço e verrugas genitais, em ambos os sexos. Esta infeção está associada a comportamentos sexuais de risco, logo os casos são superiores em indivíduos jovens, o que aumenta a importância de estudos epidemiológicos de HPV para a continua melhoria de programas eficientes de rastreio de cancro do colo do útero e de vacinação contra o HPV. Este estudo foca-se na análise das prevalências da infeção de HPV e os genótipos mais frequentes nas 9707 amostras analisadas no Centro de Medicina Laboratorial Germano de Sousa de setembro de 2019 a fevereiro de 2020. Para atingir o objetivo, calcularam-se as prevalências de infeção nas amostras de forma independente para os testes moleculares usados no Centro de Medicina Laboratorial Germano de Sousa, nomeadamente, a captura híbrida, o Teste cobas 16/18 + HR e os testes de genotipagem, CLART® HPV e Anyplex™Ⅱ HPV28. Verificou-se que a prevalência variou entre 18,4%-38,9%, dependendo do teste molecular usado. Os genótipos mais frequentes nas amostras foram o HPV16, 51, 53, 42 e 54. Neste trabalho, concluiu-se que o intervalo da prevalência da infeção por HPV é concordante com os valores obtidos nos outros estudos portugueses, os testes moleculares usados detetam os genótipos mais frequentes e os genótipos incluídos na vacina apresentam prevalências baixas na faixa etária jovem. No entanto, seria relevante estudar futuramente a inclusão dos genótipos 51 e 53 na vacina contra o HPV, uma vez que apresentam uma frequência elevada nas amostras analisadas neste estudo e não estão abrangidos na vacina atual.
ABSTRACT - HPV infection is one of the most common sexually transmitted infections in the world and high-risk HPV 16/18 accounts for about 70% of cervical cancer cases. In addition to this disease, it can also cause other anogenital cancers, head and neck cancer, and genital warts, in both sexes. This infection is associated with risky sexual behavior, so cases are superior in young individuals, which increases the importance of HPV epidemiological studies for the continued improvement of efficient cervical cancer screening and HPV vaccination programs. This study focuses on the analysis of the prevalence of HPV infection and the most frequent genotypes in the 9707 samples analyzed at the Germano de Sousa Group - Laboratory Medicine Centre from September 2019 to February 2020. To achieve the objective, the prevalence of infection in the samples was calculated independently for the molecular tests used at the Germano de Sousa Group - Laboratory Medicine Centre, namely, hybrid capture, the Cobas test 16/18 + HR, and the genotyping tests, CLART® HPV and Anyplex™Ⅱ HPV28. It was found that the prevalence varied between 18.4%-38.9%, depending on the molecular test used. The most frequent genotypes in the samples were HPV16, 51, 53, 42, and 54. In this work, it was concluded that the range of prevalence of HPV infection is concordant with the values obtained in other Portuguese studies, the molecular tests used to detect the most frequent genotypes and the genotypes included in the vaccine have a low prevalence in the young age group. However, it would be relevant to study in the future the inclusion of genotypes 51 and 53 in the HPV vaccine, since they present a high frequency in the samples analyzed in this study and are not included in the current vaccine.
ABSTRACT - HPV infection is one of the most common sexually transmitted infections in the world and high-risk HPV 16/18 accounts for about 70% of cervical cancer cases. In addition to this disease, it can also cause other anogenital cancers, head and neck cancer, and genital warts, in both sexes. This infection is associated with risky sexual behavior, so cases are superior in young individuals, which increases the importance of HPV epidemiological studies for the continued improvement of efficient cervical cancer screening and HPV vaccination programs. This study focuses on the analysis of the prevalence of HPV infection and the most frequent genotypes in the 9707 samples analyzed at the Germano de Sousa Group - Laboratory Medicine Centre from September 2019 to February 2020. To achieve the objective, the prevalence of infection in the samples was calculated independently for the molecular tests used at the Germano de Sousa Group - Laboratory Medicine Centre, namely, hybrid capture, the Cobas test 16/18 + HR, and the genotyping tests, CLART® HPV and Anyplex™Ⅱ HPV28. It was found that the prevalence varied between 18.4%-38.9%, depending on the molecular test used. The most frequent genotypes in the samples were HPV16, 51, 53, 42, and 54. In this work, it was concluded that the range of prevalence of HPV infection is concordant with the values obtained in other Portuguese studies, the molecular tests used to detect the most frequent genotypes and the genotypes included in the vaccine have a low prevalence in the young age group. However, it would be relevant to study in the future the inclusion of genotypes 51 and 53 in the HPV vaccine, since they present a high frequency in the samples analyzed in this study and are not included in the current vaccine.
Description
Mestrado em Tecnologias Moleculares em Saúde
Keywords
Frequência de genótipos Prevalência Diagnóstico molecular Rastreio Vacinação Frequency of genotypes Prevalence Molecular diagnostics Screening Vaccination
Citation
Maltinha MF. Infeção por HPV: estudo observacional das amostras do Centro de Medicina Laboratorial Germano de Sousa [dissertation]. Lisboa: Escola Superior de Tecnologia da Saúde de Lisboa/Instituto Politécnico de Lisboa; 2020.
Publisher
Instituto Politécnico de Lisboa, Escola Superior de Tecnologia da Saúde de Lisboa