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Slaughterhouses fungal burden assessment: a contribution for the pursuit of a better assessment strategy

2016-03Journal article Open access
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dc.contributor.authorViegas, Carla
dc.contributor.authorFaria, Tiago
dc.contributor.authordos Santos, Mateus
dc.contributor.authorCarolino, Elisabete
dc.contributor.authorSabino, Raquel
dc.contributor.authorQuintal Gomes, Anita
dc.contributor.authorViegas, Susana
dc.date.accessioned2016-03-10T13:47:52Z
dc.date.available2016-03-10T13:47:52Z
dc.date.issued2016-03
dc.description.abstractIn slaughterhouses, the biological risk is present not only from the direct or indirect contact with animal matter, but also from the exposure to bioaerosols. Fungal contamination was already reported from the floors and walls of slaughterhouses. This study intends to assess fungal contamination by cultural and molecular methods in poultry, swine/bovine and large animal slaughterhouses. Air samples were collected through an impaction method, while surface samples were collected by the swabbing method and subjected to further macro- and micro-scopic observations. In addition, we collected air samples using the impinger method in order to perform real-time quantitative PCR (qPCR) amplification of genes from specific fungal species, namely A. flavus, A. fumigatus and A. ochraceus complexes. Poultry and swine/bovine slaughterhouses presented each two sampling sites that surpass the guideline of 150 CFU/m3. Scopulariopsis candida was the most frequently isolated (59.5%) in poultry slaughterhouse air; Cladosporium sp. (45.7%) in the swine/bovine slaughterhouse; and Penicillium sp. (80.8%) in the large animal slaughterhouse. Molecular tools successfully amplified DNA from the A. fumigatus complex in six sampling sites where the presence of this fungal species was not identified by conventional methods. This study besides suggesting the indicators that are representative of harmful fungal contamination, also indicates a strategy as a protocol to ensure a proper characterization of fungal occupational exposure.pt_PT
dc.identifier.citationViegas C, Faria T, Santos M dos, Carolino E, Gomes AQ, Viegas S, et al. Slaughterhouses fungal burden assessment: a contribution for the pursuit of a better assessment strategy. Int J Environ Res Public Health. 2016;13(3):297.pt_PT
dc.identifier.doi10.3390/ijerph13030297pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/5823
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relation.publisherversionhttp://www.mdpi.com/1660-4601/13/3/297pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectEnvironmental healthpt_PT
dc.subjectSlaughterhousespt_PT
dc.subjectFungal burdenpt_PT
dc.subjectAssessment strategypt_PT
dc.subjectOccupational exposurept_PT
dc.subjectBioaerosolspt_PT
dc.subjectFungal contaminationpt_PT
dc.titleSlaughterhouses fungal burden assessment: a contribution for the pursuit of a better assessment strategypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.startPage297pt_PT
oaire.citation.titleInternational Journal of Environmental Research and Public Healthpt_PT
oaire.citation.volume13pt_PT
person.familyNameViegas
person.familyNameCarolino
person.familyNameSabino
person.familyNameViegas
person.givenNameCarla
person.givenNameElisabete
person.givenNameRaquel Filipa Pinheiro
person.givenNameSusana
person.identifier248817
person.identifier.ciencia-idEE1E-C639-D70F
person.identifier.ciencia-id1216-EFA3-1E0F
person.identifier.ciencia-idA919-7318-63DC
person.identifier.orcid0000-0002-1545-6479
person.identifier.orcid0000-0003-4165-7052
person.identifier.orcid0000-0001-6585-7775
person.identifier.orcid0000-0003-1015-8760
person.identifier.ridB-7217-2013
person.identifier.ridF-1012-2015
person.identifier.ridI-4053-2012
person.identifier.scopus-author-id55443609700
person.identifier.scopus-author-id25821697000
person.identifier.scopus-author-id35796067600
person.identifier.scopus-author-id35270591500
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationb5fa5da4-50c3-4b88-ae20-1bc63cb485f7
relation.isAuthorOfPublication77930d39-ed34-44dc-a4a6-9bf833e5e688
relation.isAuthorOfPublicationcf64684a-fb92-4421-91da-14fb15462fad
relation.isAuthorOfPublication13115332-43f7-4048-a8a5-2f2b855a8c92
relation.isAuthorOfPublication.latestForDiscoveryb5fa5da4-50c3-4b88-ae20-1bc63cb485f7

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