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Differential expression of CDC25 phosphatases splice variants in human breast cancer cells

2011-10Artigo científico Acesso aberto
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dc.contributor.authorAlbert, Hélène
dc.contributor.authorSantos, Susana
dc.contributor.authorBattaglia, Eric
dc.contributor.authorBrito, Miguel
dc.contributor.authorMonteiro, Carolino
dc.contributor.authorBagrel, Denyse
dc.date.accessioned2014-01-03T21:53:01Z
dc.date.available2014-01-03T21:53:01Z
dc.date.issued2011-10
dc.description.abstractBackground: CDC25 phosphatases control cell cycle progression by activating cyclin dependent kinases. The three CDC25 isoforms encoding genes are submitted to alternative splicing events which generate at least two variants for CDC25A and five for both CDC25B and CDC25C. An over-expression of CDC25 was reported in several types of cancer, including breast cancer, and is often associated with a poor prognosis. Nevertheless, most of the previous studies did not address the expression of CDC25 splice variants. Here, we evaluated CDC25 spliced transcripts expression in anti-cancerous drug-sensitive and resistant breast cancer cell lines in order to identify potential breast cancer biomarkers. Methods: CDC25 splice variants mRNA levels were evaluated by semi-quantitative RT-PCR and by an original real-time RT-PCR assay. Results: CDC25 spliced transcripts are differentially expres-sed in the breast cancer cell lines studied. An up-regulation of CDC25A2 variant and an increase of the CDC25C5/C1 ratio are associated to the multidrug-resistance in VCREMS and DOXOR breast cancer cells, compared to their sensitive counterpart cell line MCF-7. Additionally, CDC25B2 tran-script is exclusively over-expressed in VCREMS resistant cells and could therefore be involved in the development of certain type of drug resistance. Conclusions: CDC25 splice variants could represent interesting potential breast cancer prognostic biomarkers.por
dc.identifier.citationAlbert H, Santos S, Battaglia E, Brito M, Monteiro C, Bagrel D. Differential expression of CDC25 phosphatases splice variants in human breast cancer cells. Clin Chem Lab Med. 2011;49(10):1707-14.por
dc.identifier.issn1434-6621
dc.identifier.urihttp://hdl.handle.net/10400.21/3067
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherDe Gruyterpor
dc.relation.publisherversionhttp://www.degruyter.com/view/j/cclm.2011.49.issue-10/cclm.2011.635/cclm.2011.635.xml;jsessionid=F8CB5BE961C152A55085F87F901EE002por
dc.subjectAlternative splicingpor
dc.subjectBreast neoplasmspor
dc.subjectCell line, Tumorpor
dc.subjectGene expression regulationpor
dc.subjectProtein isoformspor
dc.subjectRNA splicingpor
dc.subjectReverse transcriptase polymerase chain reactionpor
dc.subjectcdc25 Phosphatasespor
dc.titleDifferential expression of CDC25 phosphatases splice variants in human breast cancer cellspor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage1714por
oaire.citation.startPage1707por
oaire.citation.titleClinical Chemistry and Laboratory Medicinepor
oaire.citation.volume49por
person.familyNameBrito
person.givenNameMiguel
person.identifier.ciencia-id231F-F341-7E93
person.identifier.orcid0000-0001-6394-658X
person.identifier.ridA-7970-2016
person.identifier.scopus-author-id35224551000
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication4252d8e0-800c-4d67-8b13-0b711d860669
relation.isAuthorOfPublication.latestForDiscovery4252d8e0-800c-4d67-8b13-0b711d860669

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