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Fungal burden in filtering respiratory protective devices used in the waste sorting industry

2019-06Conference object Open access
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dc.contributor.authorAlmeida, Beatriz
dc.contributor.authorTwarużek, M.
dc.contributor.authorKosicki, R.
dc.contributor.authorGrajewski, J.
dc.contributor.authorViegas, Carla
dc.date.accessioned2019-07-22T17:50:01Z
dc.date.available2019-07-22T17:50:01Z
dc.date.issued2019-06
dc.descriptionProject EXPOsE - Project nº 23222 (02/SAICT/2016). Project IPL/2018/WasteFRPD_ESTeSL.pt_PT
dc.description.abstractOne of the solutions for reducing the workers' exposure to the effects of organic dust is the use of Filtering Respiratory Protective Devices (FRPD). During FFR use, water vapor and sweat are released increasing humidity of the material providing favorable conditions for fungal growth. The aim of this study is to characterize the fungal burden (fungi and mycotoxins) retained in FRPD used by workers from one waste sorting from Portugal. Fifty-four FFRs (Protection FFP3) were collected after normal use (one work shift) from waste sorting workers. The exhalation valve and 2 cm2 from the interior layer of the each FFR were extracted and seeded on two media: 2% malt extract agar (MEA) supplemented with 0.05 g/L chloramphenicol and dichloran-glycerol agar (DG18), following incubation at 27 °C for 5–7 days. All FFRs samples will be screened for mycotoxins presence. The fungal contamination in the interior layer of the mask ranged from 0 to 25 CFU.cm-2 in MEA, and from 0 to 26.4 CFU.cm-2 in DG18. Six different fungal species were found in the interior layer in both MEA and DG18. The most common fungal genera found in MEA were Lichtheima (57.41%), Penicillium (27.10%) and Aspergillus (14.35%; including sections Fumigati, Nigri, Flavi, Candidi, and Cicumdati). In DG18, the most common genera were Penicillium (85.37%), Aspergillus (14.29%; comprising sections Fumigati, Circumdati, Candidi, Flavi, Nigri, and Aspergilli) and Mucor sp. (0.15%). In the exhalation valve, the fungal contamination ranged from 0 to 0.45 CFU.cm-2 in MEA, and from 0 to 0.8 CFU.cm-2 in DG18. In MEA, only two genera were found: Penicillium (60.53%) and Aspergillus (39.47%; including sections Fumigati and Nigri). But in DG18, seven different genera were found, of which the most found were Penicillium (68%), Aspergillus (25.33%; covering sections Fumigati, Candidi, Nigri, Restricti, and Aspergilli) and Mucor (2.67%). Our results point out for the need for intervention regarding the FFR replacement frequency due to quantitative and qualitative results (species with toxigenic potential).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAlmeida B, Santos S, Twarużek M, Kosicki R, Viegas S, Viegas C. Fungal burden in filtering respiratory protective devices used in the waste sorting industry. In: 12th International Conference Mycotoxins and Moulds – Current trends, Bydgoszcz (Poland), June 26-27, 2019.pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/10354
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationProject nº 23222 (02/SAICT/2016)pt_PT
dc.relationProject EXPOsEpt_PT
dc.relationIPL/2018/WasteFRPD_ESTeSLpt_PT
dc.relation.publisherversionhttps://mycotoxin.ukw.edu.pl/jednostka/mycotoxins-and-moulds-2019/programpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectOccupational healthpt_PT
dc.subjectOccupational exposurept_PT
dc.subjectWaste sorting industrypt_PT
dc.subjectFungipt_PT
dc.subjectFungal burdenpt_PT
dc.subjectFiltering respiratory protective devicespt_PT
dc.subjectProject nº 23222 (02/SAICT/2016)pt_PT
dc.subjectProject EXPOsEpt_PT
dc.subjectIPL/2018/WasteFRPD_ESTeSLpt_PT
dc.titleFungal burden in filtering respiratory protective devices used in the waste sorting industrypt_PT
dc.typeconference object
dspace.entity.typePublication
person.familyNameViegas
person.givenNameCarla
person.identifier.ciencia-idEE1E-C639-D70F
person.identifier.orcid0000-0002-1545-6479
person.identifier.ridB-7217-2013
person.identifier.scopus-author-id55443609700
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublicationb5fa5da4-50c3-4b88-ae20-1bc63cb485f7
relation.isAuthorOfPublication.latestForDiscoveryb5fa5da4-50c3-4b88-ae20-1bc63cb485f7

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