Repository logo
 
Publication

Use of a-SiC:H semiconductor-based transducer for glucose sensing through FRET analysis

2013Conference object Open access
Simple item page
dc.contributor.authorLouro, Paula
dc.contributor.authorVaz da Silva, V
dc.contributor.authorVieira, Manuel Augusto
dc.contributor.authorKarmali, Amin
dc.contributor.authorVieira, Manuela
dc.date.accessioned2013-10-20T18:52:50Z
dc.date.available2013-10-20T18:52:50Z
dc.date.issued2013
dc.description.abstractGlucose sensing is an issue with great interest in medical and biological applications. One possible approach to glucose detection takes advantage of measuring changes in fluorescence resonance energy transfer (FRET) between a fluorescent donor and an acceptor within a protein which undergoes glucose-induced changes in conformation. This demands the detection of fluorescent signals in the visible spectrum. In this paper we analyzed the emission spectrum obtained from fluorescent labels attached to a protein which changes its conformation in the presence of glucose using a commercial spectrofluorometer. Different glucose nanosensors were used to measure the output spectra with fluorescent signals located at the cyan and yellow bands of the spectrum. A new device is presented based on multilayered a-SiC:H heterostructures to detect identical transient visible signals. The transducer consists of a p-i'(a-SiC:H)-n/p-i(a-Si:H)-n heterostructure optimized for the detection of the fluorescence resonance energy transfer between fluorophores with excitation in the violet (400 nm) and emissions in the cyan (470 nm) and yellow (588 nm) range of the spectrum. Results show that the device photocurrent signal measured under reverse bias and using appropriate steady state optical bias, allows the separate detection of the cyan and yellow fluorescence signals presented.por
dc.identifier.citationLOURO, Paula; [et al] – Use of a-SiC:H semiconductor-based transducer for glucose sensing through FRET analysis. In Technological Innovation for the Internet of Things. ISBN 978-642-37290-2. Vol. 394 (2013), pp. 631-638.por
dc.identifier.isbn978-3-642-37290-2
dc.identifier.issn1868-4238
dc.identifier.urihttp://hdl.handle.net/10400.21/2759
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherSpringer-Verlag Berlinpor
dc.relation.ispartofseriesIFIP Advances in Information and Communication Technology
dc.subjectGlucose sensingpor
dc.subjectFRETpor
dc.subjectNanosensorspor
dc.subjectFluorescence proteinspor
dc.subjecta-SiC:H photodiodespor
dc.subjectMultilayered heterostructurespor
dc.subjectOptical sensorspor
dc.titleUse of a-SiC:H semiconductor-based transducer for glucose sensing through FRET analysispor
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlaceBerlinpor
oaire.citation.endPage638por
oaire.citation.startPage631por
oaire.citation.titleTechnological Innovation for the Internet of Thingspor
oaire.citation.volume394por
person.familyNameLouro
person.familyNameVaz da Silva
person.familyNameAugusto Vieira
person.familyNameKarmali
person.familyNameVieira
person.givenNamePaula
person.givenNameVitor
person.givenNameManuel
person.givenNameAmin
person.givenNameManuela
person.identifier499161
person.identifierI-8527-2018
person.identifier10792
person.identifier.ciencia-idE511-8C08-E606
person.identifier.ciencia-idAB18-740E-B580
person.identifier.ciencia-idA511-1330-549F
person.identifier.ciencia-id9516-E25E-BB8E
person.identifier.orcid0000-0002-4167-2052
person.identifier.orcid0000-0003-4265-6553
person.identifier.orcid0000-0003-1385-3646
person.identifier.orcid0000-0003-0419-401X
person.identifier.orcid0000-0002-1150-9895
person.identifier.ridU-8346-2017
person.identifier.ridE-4787-2011
person.identifier.ridV-7860-2017
person.identifier.scopus-author-id8845716400
person.identifier.scopus-author-id5719 1567905
person.identifier.scopus-author-id6603778216
person.identifier.scopus-author-id7202140173
rcaap.rightsrestrictedAccesspor
rcaap.typeconferenceObjectpor
relation.isAuthorOfPublication730dc3dd-73eb-428d-b3a6-430085e33585
relation.isAuthorOfPublicationbf73931d-fd0b-44bc-82a9-fdfd2157406f
relation.isAuthorOfPublicationd8ffd958-0f70-4ec1-a9e9-d18e5c6d308e
relation.isAuthorOfPublication23975b4b-3234-4139-9675-89ea6f9a2332
relation.isAuthorOfPublicationb77c6785-9cfb-4cd4-90b5-c2b816bd7d11
relation.isAuthorOfPublication.latestForDiscoverybf73931d-fd0b-44bc-82a9-fdfd2157406f

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Use_of_a-SiCH_PLouro.pdf
Size:
523.83 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

ISEL - Eng. Elect. Tel. Comp. - Comunicações